Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.

Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Teng Li, Hua Liu, Nan Jiang, Yiluo Wang, Ying Wang, Jing Zhang, Yujuan Shen, Jianping Cao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d375035e32f348329b3ef35bda0c9eb8
record_format	dspace
spelling	oai:doaj.org-article:d375035e32f348329b3ef35bda0c9eb82021-12-02T20:24:13ZComparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.1935-27271935-273510.1371/journal.pntd.0009949https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb82021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009949https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptosporidiosis. In the present study, to better understand the detailed interaction between the host and Cryptosporidium parvum, a large-scale label-free proteomics study was conducted to characterize the changes to the proteome induced by C. parvum infection. Among 4406 proteins identified, 121 proteins were identified as differentially abundant (> 1.5-fold cutoff, P < 0.05) in C. parvum infected HCT-8 cells compared with uninfected cells. Among them, 67 proteins were upregulated, and 54 proteins were downregulated at 36 h post infection. Analysis of the differentially abundant proteins revealed an interferon-centered immune response of the host cells against C. parvum infection and extensive inhibition of metabolism-related enzymes in the host cells caused by infection. Several proteins were further verified using quantitative real-time reverse transcription polymerase chain reaction and western blotting. This systematic analysis of the proteomics of C. parvum-infected HCT-8 cells identified a wide range of functional proteins that participate in host anti-parasite immunity or act as potential targets during infection, providing new insights into the molecular mechanism of C. parvum infection.Teng LiHua LiuNan JiangYiluo WangYing WangJing ZhangYujuan ShenJianping CaoPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009949 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
description	Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptosporidiosis. In the present study, to better understand the detailed interaction between the host and Cryptosporidium parvum, a large-scale label-free proteomics study was conducted to characterize the changes to the proteome induced by C. parvum infection. Among 4406 proteins identified, 121 proteins were identified as differentially abundant (> 1.5-fold cutoff, P < 0.05) in C. parvum infected HCT-8 cells compared with uninfected cells. Among them, 67 proteins were upregulated, and 54 proteins were downregulated at 36 h post infection. Analysis of the differentially abundant proteins revealed an interferon-centered immune response of the host cells against C. parvum infection and extensive inhibition of metabolism-related enzymes in the host cells caused by infection. Several proteins were further verified using quantitative real-time reverse transcription polymerase chain reaction and western blotting. This systematic analysis of the proteomics of C. parvum-infected HCT-8 cells identified a wide range of functional proteins that participate in host anti-parasite immunity or act as potential targets during infection, providing new insights into the molecular mechanism of C. parvum infection.
format	article
author	Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao
author_facet	Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao
author_sort	Teng Li
title	Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
title_short	Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
title_full	Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
title_fullStr	Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
title_full_unstemmed	Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
title_sort	comparative proteomics reveals cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8
work_keys_str_mv	AT tengli comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT hualiu comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT nanjiang comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT yiluowang comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT yingwang comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT jingzhang comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT yujuanshen comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse AT jianpingcao comparativeproteomicsrevealscryptosporidiumparvummanipulationofthehostcellmolecularexpressionandimmuneresponse
_version_	1718374077252501504

Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.

Ejemplares similares