Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice

BRV and GVBaV are the most important viruses affecting currant production worldwide. The subject of this work was to study genetic variability of Blackcurrant reversion virus (BRV) and Gooseberry vein banding associated virus (GVBaV) in the Czech Republic using 3’UTR and ORF 3 regions, respectively....

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Autor principal:	Josef Podlipný
Formato:	article
Lenguaje:	CS EN
Publicado:	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o. 2021
Materias:	brv gvbav currant rt-pcr Plant culture SB1-1110 Botany QK1-989
Acceso en línea:	https://doaj.org/article/d3765e7113f7470ba6591d7b061406c6
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spelling	oai:doaj.org-article:d3765e7113f7470ba6591d7b061406c62021-11-22T06:15:14ZGenetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice2695-1347https://doaj.org/article/d3765e7113f7470ba6591d7b061406c62021-11-01T00:00:00Zhttps://vpovsuo.cz/wp-content/uploads/2021/11/2_Podlipny_VPO-27_2_21.pdfhttps://doaj.org/toc/2695-1347BRV and GVBaV are the most important viruses affecting currant production worldwide. The subject of this work was to study genetic variability of Blackcurrant reversion virus (BRV) and Gooseberry vein banding associated virus (GVBaV) in the Czech Republic using 3’UTR and ORF 3 regions, respectively. In total, 155 sequences were obtained (74 for BRV; 81 for GVBaV) from a set of 103 BRV and 82 GVBaV RT-PCR-positive samples. Based on phylogenetic trees created in a MEGA X program, 38 different BRV genotypes and 30 GVBaV genotypes were defined. In both viruses, genotypes could be divided into two main clusters (A and B), and further subdivided into A1 and A2 subgroups, and B1 and B2 subgroups for BRV only. The most common BRV genotype was B2.16 (33.8 % of BRV-infected plants), and the GVBaV genotype B.4 (26.3 % of GVBaV-infected plants). Overall, an intraspecies variability in BRV was higher than in GVBaV that was generally low. BRV was found most frequently in production fields (84.2 % positive plants), while GVBaV was identified predominantly in non-production plantings. Black currant was the most frequent host of BRV, while GVBaV was mostly identified in red and white currants.Josef PodlipnýVÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.articlebrvgvbavcurrantrt-pcrPlant cultureSB1-1110BotanyQK1-989CSENVědecké Práce Ovocnářské, Vol 27, Iss 2, Pp 14-24 (2021)
institution	DOAJ
collection	DOAJ
language	CS EN
topic	brv gvbav currant rt-pcr Plant culture SB1-1110 Botany QK1-989
spellingShingle	brv gvbav currant rt-pcr Plant culture SB1-1110 Botany QK1-989 Josef Podlipný Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
description	BRV and GVBaV are the most important viruses affecting currant production worldwide. The subject of this work was to study genetic variability of Blackcurrant reversion virus (BRV) and Gooseberry vein banding associated virus (GVBaV) in the Czech Republic using 3’UTR and ORF 3 regions, respectively. In total, 155 sequences were obtained (74 for BRV; 81 for GVBaV) from a set of 103 BRV and 82 GVBaV RT-PCR-positive samples. Based on phylogenetic trees created in a MEGA X program, 38 different BRV genotypes and 30 GVBaV genotypes were defined. In both viruses, genotypes could be divided into two main clusters (A and B), and further subdivided into A1 and A2 subgroups, and B1 and B2 subgroups for BRV only. The most common BRV genotype was B2.16 (33.8 % of BRV-infected plants), and the GVBaV genotype B.4 (26.3 % of GVBaV-infected plants). Overall, an intraspecies variability in BRV was higher than in GVBaV that was generally low. BRV was found most frequently in production fields (84.2 % positive plants), while GVBaV was identified predominantly in non-production plantings. Black currant was the most frequent host of BRV, while GVBaV was mostly identified in red and white currants.
format	article
author	Josef Podlipný
author_facet	Josef Podlipný
author_sort	Josef Podlipný
title	Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
title_short	Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
title_full	Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
title_fullStr	Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
title_full_unstemmed	Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice
title_sort	genetická variabilita virů rybízu blackcurrant reversion virus (brv) a gooseberry vein banding associated virus (gvbav) v české republice
publisher	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
publishDate	2021
url	https://doaj.org/article/d3765e7113f7470ba6591d7b061406c6
work_keys_str_mv	AT josefpodlipny genetickavariabilitavirurybizublackcurrantreversionvirusbrvagooseberryveinbandingassociatedvirusgvbavvceskerepublice
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Genetická variabilita virů rybízu Blackcurrant reversion virus (BRV) a Gooseberry vein banding associated virus (GVBaV) v České republice

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