Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey.
West Nile virus (WNV), a mosquito-borne flavivirus with significant impact on human and animal health, has recently demonstrated an expanded zone of activity globally. The aim of this study is to investigate the frequency and distribution of WNV infections in potential vectors and several mammal and...
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2014
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oai:doaj.org-article:d37fecc54db34653a8fef53929658e752021-11-25T06:32:21ZSerological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey.1935-27271935-273510.1371/journal.pntd.0003028https://doaj.org/article/d37fecc54db34653a8fef53929658e752014-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25058465/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735West Nile virus (WNV), a mosquito-borne flavivirus with significant impact on human and animal health, has recently demonstrated an expanded zone of activity globally. The aim of this study is to investigate the frequency and distribution of WNV infections in potential vectors and several mammal and avian species in Turkey, where previous data indicate viral circulation. The study was conducted in 15 provinces across Turkey during 2011-2013. In addition, the entomological study was extended to 4 districts of the Turkish Republic of Northern Cyprus. WNV exposure was determined in humans, horses, sheep and ducks from Mersin, Sanliurfa, Van and Kars provinces of Turkey, via the detection of neutralizing antibodies. WNV RNA was sought in human and equine samples from Mersin, Adana and Mugla provinces. Field-collected mosquitoes from 92 sites at 46 locations were characterized morphologically and evaluated for viral RNA. Neutralizing antibodies were identified in 10.5% of the 1180 samples studied and detected in all species evaluated. Viral nucleic acids were observed in 5.9% of 522 samples but only in horses. A total of 2642 mosquito specimens belonging to 15 species were captured, where Ochlerotatus caspius (52.4%), Culex pipiens sensu lato (24.2%) comprise the most frequent species. WNV RNA was detected in 4 mosquito pools (1.9%), that comprise Oc. caspius Cx. pipiens s.l. and DNA barcoding revealed the presence of Cx. quinquefasciatus and Cx. perexiguus mosquitoes in infected Culex pools. All WNV partial sequences were characterized as lineage 1 clade 1a. These findings indicate a widespread WNV activity in Turkey, in Eastern Thrace and Mediterranean-Aegean regions as well as Southeastern and Northeastern Anatolia.Koray ErgunayFiliz GunayOzge Erisoz KasapKerem OterSepandar GargariTaner KaraogluSeda TezcanMehmet CabalarYakup YildirimGürol EmekdasBulent AltenAykut OzkulPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3028 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
| spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Koray Ergunay Filiz Gunay Ozge Erisoz Kasap Kerem Oter Sepandar Gargari Taner Karaoglu Seda Tezcan Mehmet Cabalar Yakup Yildirim Gürol Emekdas Bulent Alten Aykut Ozkul Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| description |
West Nile virus (WNV), a mosquito-borne flavivirus with significant impact on human and animal health, has recently demonstrated an expanded zone of activity globally. The aim of this study is to investigate the frequency and distribution of WNV infections in potential vectors and several mammal and avian species in Turkey, where previous data indicate viral circulation. The study was conducted in 15 provinces across Turkey during 2011-2013. In addition, the entomological study was extended to 4 districts of the Turkish Republic of Northern Cyprus. WNV exposure was determined in humans, horses, sheep and ducks from Mersin, Sanliurfa, Van and Kars provinces of Turkey, via the detection of neutralizing antibodies. WNV RNA was sought in human and equine samples from Mersin, Adana and Mugla provinces. Field-collected mosquitoes from 92 sites at 46 locations were characterized morphologically and evaluated for viral RNA. Neutralizing antibodies were identified in 10.5% of the 1180 samples studied and detected in all species evaluated. Viral nucleic acids were observed in 5.9% of 522 samples but only in horses. A total of 2642 mosquito specimens belonging to 15 species were captured, where Ochlerotatus caspius (52.4%), Culex pipiens sensu lato (24.2%) comprise the most frequent species. WNV RNA was detected in 4 mosquito pools (1.9%), that comprise Oc. caspius Cx. pipiens s.l. and DNA barcoding revealed the presence of Cx. quinquefasciatus and Cx. perexiguus mosquitoes in infected Culex pools. All WNV partial sequences were characterized as lineage 1 clade 1a. These findings indicate a widespread WNV activity in Turkey, in Eastern Thrace and Mediterranean-Aegean regions as well as Southeastern and Northeastern Anatolia. |
| format |
article |
| author |
Koray Ergunay Filiz Gunay Ozge Erisoz Kasap Kerem Oter Sepandar Gargari Taner Karaoglu Seda Tezcan Mehmet Cabalar Yakup Yildirim Gürol Emekdas Bulent Alten Aykut Ozkul |
| author_facet |
Koray Ergunay Filiz Gunay Ozge Erisoz Kasap Kerem Oter Sepandar Gargari Taner Karaoglu Seda Tezcan Mehmet Cabalar Yakup Yildirim Gürol Emekdas Bulent Alten Aykut Ozkul |
| author_sort |
Koray Ergunay |
| title |
Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| title_short |
Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| title_full |
Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| title_fullStr |
Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| title_full_unstemmed |
Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. |
| title_sort |
serological, molecular and entomological surveillance demonstrates widespread circulation of west nile virus in turkey. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/d37fecc54db34653a8fef53929658e75 |
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