IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.

Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed i...

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Autores principales:	Bo Chen, Gengzhen Zhu, An Yan, Jing He, Yang Liu, Lin Li, Xuerui Yang, Chen Dong, Kehkooi Kee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d38d544322054ec993d85cad0a8e0a6f
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spelling	oai:doaj.org-article:d38d544322054ec993d85cad0a8e0a6f2021-12-02T20:03:21ZIGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.1553-73901553-740410.1371/journal.pgen.1009778https://doaj.org/article/d38d544322054ec993d85cad0a8e0a6f2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009778https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed in both Sertoli cells and germ cells, leads to male infertility in mice. Combining a new meiotic fluorescent reporter system with testicular cell transplantation, we demonstrated that IGSF11 is required in both somatic cells and spermatogenic cells for primary spermatocyte development. In the absence of IGSF11, spermatocytes proceed through pachytene, but the pericentric heterochromatin of nonhomologous chromosomes remains inappropriately clustered from late pachytene onward, resulting in undissolved interchromosomal interactions. Hi-C analysis reveals elevated levels of interchromosomal interactions occurring mostly at the chromosome ends. Collectively, our data elucidates that IGSF11 in somatic cells and germ cells is required for pericentric heterochromatin dissociation during diplotene in mouse primary spermatocytes.Bo ChenGengzhen ZhuAn YanJing HeYang LiuLin LiXuerui YangChen DongKehkooi KeePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009778 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Bo Chen Gengzhen Zhu An Yan Jing He Yang Liu Lin Li Xuerui Yang Chen Dong Kehkooi Kee IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
description	Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed in both Sertoli cells and germ cells, leads to male infertility in mice. Combining a new meiotic fluorescent reporter system with testicular cell transplantation, we demonstrated that IGSF11 is required in both somatic cells and spermatogenic cells for primary spermatocyte development. In the absence of IGSF11, spermatocytes proceed through pachytene, but the pericentric heterochromatin of nonhomologous chromosomes remains inappropriately clustered from late pachytene onward, resulting in undissolved interchromosomal interactions. Hi-C analysis reveals elevated levels of interchromosomal interactions occurring mostly at the chromosome ends. Collectively, our data elucidates that IGSF11 in somatic cells and germ cells is required for pericentric heterochromatin dissociation during diplotene in mouse primary spermatocytes.
format	article
author	Bo Chen Gengzhen Zhu An Yan Jing He Yang Liu Lin Li Xuerui Yang Chen Dong Kehkooi Kee
author_facet	Bo Chen Gengzhen Zhu An Yan Jing He Yang Liu Lin Li Xuerui Yang Chen Dong Kehkooi Kee
author_sort	Bo Chen
title	IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
title_short	IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
title_full	IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
title_fullStr	IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
title_full_unstemmed	IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
title_sort	igsf11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d38d544322054ec993d85cad0a8e0a6f
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_version_	1718375689498918912

IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.

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