Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages

Abstract In poultry, in vitro propagated primordial germ cells (PGCs) represent an important tool for the cryopreservation of avian genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs during in vitro propagation, which may compromise their reproductive c...

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Autores principales:	Laura Soler, Sabine Alves, Aurélien Brionne, Aurore Jacques, Vanessa Guérin, Maeva Cherif-Feildel, Lucie Combes-Soia, Sophie Fouchécourt, Aurore Thélie, Elisabeth Blesbois, Michael J. McGrew, Valérie Labas, Marina S. Govoroun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d391d850babd4894b90fd60e688afe75
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spelling	oai:doaj.org-article:d391d850babd4894b90fd60e688afe752021-12-02T19:16:47ZProtein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages10.1038/s41598-021-98454-22045-2322https://doaj.org/article/d391d850babd4894b90fd60e688afe752021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98454-2https://doaj.org/toc/2045-2322Abstract In poultry, in vitro propagated primordial germ cells (PGCs) represent an important tool for the cryopreservation of avian genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs during in vitro propagation, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal migratory male (ZZ) and female (ZW) chicken PGCs propagated in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. Many proteins were found to be differentially abundant in chicken male and female PGCs indicating their early sexual identity. Many of the proteins more highly expressed in male PGCs were encoded by genes localised to the Z sex chromosome. This suggests that the known lack of dosage compensation of the transcription of Z-linked genes between sexes persists at the protein level in PGCs, and that this may be a key factor of their autonomous sex differentiation. We also found that globally, protein differences do not closely correlate with transcript differences indicating a selective translational mechanism in PGCs. Male and female PGC expressed protein sets were associated with differential biological processes and contained proteins known to be biologically relevant for male and female germ cell development, respectively. We also discovered that female PGCs have a higher capacity to uptake proteins from the cell culture medium than male PGCs. This study presents the first evidence of an early predetermined sex specific cell fate of chicken PGCs and their sexual molecular specificities which will enable the development of more precise sex-specific in vitro culture conditions for the preservation of avian genetic resources.Laura SolerSabine AlvesAurélien BrionneAurore JacquesVanessa GuérinMaeva Cherif-FeildelLucie Combes-SoiaSophie FouchécourtAurore ThélieElisabeth BlesboisMichael J. McGrewValérie LabasMarina S. GovorounNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Soler Sabine Alves Aurélien Brionne Aurore Jacques Vanessa Guérin Maeva Cherif-Feildel Lucie Combes-Soia Sophie Fouchécourt Aurore Thélie Elisabeth Blesbois Michael J. McGrew Valérie Labas Marina S. Govoroun Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
description	Abstract In poultry, in vitro propagated primordial germ cells (PGCs) represent an important tool for the cryopreservation of avian genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs during in vitro propagation, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal migratory male (ZZ) and female (ZW) chicken PGCs propagated in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. Many proteins were found to be differentially abundant in chicken male and female PGCs indicating their early sexual identity. Many of the proteins more highly expressed in male PGCs were encoded by genes localised to the Z sex chromosome. This suggests that the known lack of dosage compensation of the transcription of Z-linked genes between sexes persists at the protein level in PGCs, and that this may be a key factor of their autonomous sex differentiation. We also found that globally, protein differences do not closely correlate with transcript differences indicating a selective translational mechanism in PGCs. Male and female PGC expressed protein sets were associated with differential biological processes and contained proteins known to be biologically relevant for male and female germ cell development, respectively. We also discovered that female PGCs have a higher capacity to uptake proteins from the cell culture medium than male PGCs. This study presents the first evidence of an early predetermined sex specific cell fate of chicken PGCs and their sexual molecular specificities which will enable the development of more precise sex-specific in vitro culture conditions for the preservation of avian genetic resources.
format	article
author	Laura Soler Sabine Alves Aurélien Brionne Aurore Jacques Vanessa Guérin Maeva Cherif-Feildel Lucie Combes-Soia Sophie Fouchécourt Aurore Thélie Elisabeth Blesbois Michael J. McGrew Valérie Labas Marina S. Govoroun
author_facet	Laura Soler Sabine Alves Aurélien Brionne Aurore Jacques Vanessa Guérin Maeva Cherif-Feildel Lucie Combes-Soia Sophie Fouchécourt Aurore Thélie Elisabeth Blesbois Michael J. McGrew Valérie Labas Marina S. Govoroun
author_sort	Laura Soler
title	Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
title_short	Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
title_full	Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
title_fullStr	Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
title_full_unstemmed	Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
title_sort	protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d391d850babd4894b90fd60e688afe75
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Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages

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