Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.

Progressive increases in ethanol consumption is a hallmark of alcohol use disorder (AUD). Persistent changes in brain gene expression are hypothesized to underlie the altered neural signaling producing abusive consumption in AUD. To identify brain regional gene expression networks contributing to pr...

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Autores principales:	Maren L Smith, Marcelo F Lopez, Aaron R Wolen, Howard C Becker, Michael F Miles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3a8f1a18fdb4a32bbaec0a6f6ab9f4a
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spelling	oai:doaj.org-article:d3a8f1a18fdb4a32bbaec0a6f6ab9f4a2021-12-02T20:11:29ZBrain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.1932-620310.1371/journal.pone.0233319https://doaj.org/article/d3a8f1a18fdb4a32bbaec0a6f6ab9f4a2020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0233319https://doaj.org/toc/1932-6203Progressive increases in ethanol consumption is a hallmark of alcohol use disorder (AUD). Persistent changes in brain gene expression are hypothesized to underlie the altered neural signaling producing abusive consumption in AUD. To identify brain regional gene expression networks contributing to progressive ethanol consumption, we performed microarray and scale-free network analysis of expression responses in a C57BL/6J mouse model utilizing chronic intermittent ethanol by vapor chamber (CIE) in combination with limited access oral ethanol consumption. This model has previously been shown to produce long-lasting increased ethanol consumption, particularly when combining oral ethanol access with repeated cycles of intermittent vapor exposure. The interaction of CIE and oral consumption was studied by expression profiling and network analysis in medial prefrontal cortex, nucleus accumbens, hippocampus, bed nucleus of the stria terminalis, and central nucleus of the amygdala. Brain region expression networks were analyzed for ethanol-responsive gene expression, correlation with ethanol consumption and functional content using extensive bioinformatics studies. In all brain-regions studied the largest number of changes in gene expression were seen when comparing ethanol naïve mice to those exposed to CIE and drinking. In the prefrontal cortex, however, unique patterns of gene expression were seen compared to other brain-regions. Network analysis identified modules of co-expressed genes in all brain regions. The prefrontal cortex and nucleus accumbens showed the greatest number of modules with significant correlation to drinking behavior. Across brain-regions, however, many modules with strong correlations to drinking, both baseline intake and amount consumed after CIE, showed functional enrichment for synaptic transmission and synaptic plasticity.Maren L SmithMarcelo F LopezAaron R WolenHoward C BeckerMichael F MilesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 5, p e0233319 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maren L Smith Marcelo F Lopez Aaron R Wolen Howard C Becker Michael F Miles Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
description	Progressive increases in ethanol consumption is a hallmark of alcohol use disorder (AUD). Persistent changes in brain gene expression are hypothesized to underlie the altered neural signaling producing abusive consumption in AUD. To identify brain regional gene expression networks contributing to progressive ethanol consumption, we performed microarray and scale-free network analysis of expression responses in a C57BL/6J mouse model utilizing chronic intermittent ethanol by vapor chamber (CIE) in combination with limited access oral ethanol consumption. This model has previously been shown to produce long-lasting increased ethanol consumption, particularly when combining oral ethanol access with repeated cycles of intermittent vapor exposure. The interaction of CIE and oral consumption was studied by expression profiling and network analysis in medial prefrontal cortex, nucleus accumbens, hippocampus, bed nucleus of the stria terminalis, and central nucleus of the amygdala. Brain region expression networks were analyzed for ethanol-responsive gene expression, correlation with ethanol consumption and functional content using extensive bioinformatics studies. In all brain-regions studied the largest number of changes in gene expression were seen when comparing ethanol naïve mice to those exposed to CIE and drinking. In the prefrontal cortex, however, unique patterns of gene expression were seen compared to other brain-regions. Network analysis identified modules of co-expressed genes in all brain regions. The prefrontal cortex and nucleus accumbens showed the greatest number of modules with significant correlation to drinking behavior. Across brain-regions, however, many modules with strong correlations to drinking, both baseline intake and amount consumed after CIE, showed functional enrichment for synaptic transmission and synaptic plasticity.
format	article
author	Maren L Smith Marcelo F Lopez Aaron R Wolen Howard C Becker Michael F Miles
author_facet	Maren L Smith Marcelo F Lopez Aaron R Wolen Howard C Becker Michael F Miles
author_sort	Maren L Smith
title	Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
title_short	Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
title_full	Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
title_fullStr	Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
title_full_unstemmed	Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
title_sort	brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/d3a8f1a18fdb4a32bbaec0a6f6ab9f4a
work_keys_str_mv	AT marenlsmith brainregionalgeneexpressionnetworkanalysisidentifiesuniqueinteractionsbetweenchronicethanolexposureandconsumption AT marceloflopez brainregionalgeneexpressionnetworkanalysisidentifiesuniqueinteractionsbetweenchronicethanolexposureandconsumption AT aaronrwolen brainregionalgeneexpressionnetworkanalysisidentifiesuniqueinteractionsbetweenchronicethanolexposureandconsumption AT howardcbecker brainregionalgeneexpressionnetworkanalysisidentifiesuniqueinteractionsbetweenchronicethanolexposureandconsumption AT michaelfmiles brainregionalgeneexpressionnetworkanalysisidentifiesuniqueinteractionsbetweenchronicethanolexposureandconsumption
_version_	1718374912310116352

Brain regional gene expression network analysis identifies unique interactions between chronic ethanol exposure and consumption.

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