Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone

Regardless of the advancement of synthetic bone substitutes, allograft-derived bone substitutes still dominate in the orthopaedic circle in the treatments of bone diseases. Nevertheless, the stringent devitalization process jeopardizes their osseointegration with host bone and therefore prone to lon...

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Autores principales:	Wenhao Wang, Jie Shen, Yuan Meng, Miaoman Ye, Shaozhang Lin, Qi Zhao, Le Wang, Kenneth M.C. Cheung, Shuilin Wu, Yufeng Zheng, Xuanyong Liu, Paul K. Chu, Kelvin W.K. Yeung, Zhi-Yong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Osseointegration Allograft bone Bone tissue microenvironment Bone-implant interface Magnesium Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d3b388df26b84375a019dc73a2d5cf3c
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spelling	oai:doaj.org-article:d3b388df26b84375a019dc73a2d5cf3c2021-11-26T04:36:57ZMagnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone2452-199X10.1016/j.bioactmat.2021.08.027https://doaj.org/article/d3b388df26b84375a019dc73a2d5cf3c2022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004035https://doaj.org/toc/2452-199XRegardless of the advancement of synthetic bone substitutes, allograft-derived bone substitutes still dominate in the orthopaedic circle in the treatments of bone diseases. Nevertheless, the stringent devitalization process jeopardizes their osseointegration with host bone and therefore prone to long-term failure. Hence, improving osseointegration and transplantation efficiency remains important. The alteration of bone tissue microenvironment (TME) to facilitate osseointegration has been generally recognized. However, the concept of exerting metal ionic cue in bone TME without compromising the mechanical properties of bone allograft is challenging. To address this concern, an interfacial tissue microenvironment with magnesium cationc cue was tailored onto the gamma-irradiated allograft bone using a customized magnesium-plasma surface treatment. The formation of the Mg cationic cue enriched interfacial tissue microenvironment on allograft bone was verified by the scanning ion-selective electrode technique. The cellular activities of human TERT-immortalized mesenchymal stem cells on the Mg-enriched grafts were notably upregulated. In the animal test, superior osseointegration between Mg-enriched graft and host bone was found, whereas poor integration was observed in the gamma-irradiated controls at 28 days post-operation. Furthermore, the bony in-growth appeared on magnesium-enriched allograft bone was significant higher. The mechanism possibly correlates to the up-regulation of integrin receptors in mesenchymal stem cells under modified bone TME that directly orchestrate the initial cell attachment and osteogenic differentiation of mesenchymal stem cells. Lastly, our findings demonstrate the significance of magnesium cation modified bone allograft that can potentially translate to various orthopaedic procedures requiring bone augmentation.Wenhao WangJie ShenYuan MengMiaoman YeShaozhang LinQi ZhaoLe WangKenneth M.C. CheungShuilin WuYufeng ZhengXuanyong LiuPaul K. ChuKelvin W.K. YeungZhi-Yong ZhangKeAi Communications Co., Ltd.articleOsseointegrationAllograft boneBone tissue microenvironmentBone-implant interfaceMagnesiumMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 32-47 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osseointegration Allograft bone Bone tissue microenvironment Bone-implant interface Magnesium Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Osseointegration Allograft bone Bone tissue microenvironment Bone-implant interface Magnesium Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Wenhao Wang Jie Shen Yuan Meng Miaoman Ye Shaozhang Lin Qi Zhao Le Wang Kenneth M.C. Cheung Shuilin Wu Yufeng Zheng Xuanyong Liu Paul K. Chu Kelvin W.K. Yeung Zhi-Yong Zhang Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
description	Regardless of the advancement of synthetic bone substitutes, allograft-derived bone substitutes still dominate in the orthopaedic circle in the treatments of bone diseases. Nevertheless, the stringent devitalization process jeopardizes their osseointegration with host bone and therefore prone to long-term failure. Hence, improving osseointegration and transplantation efficiency remains important. The alteration of bone tissue microenvironment (TME) to facilitate osseointegration has been generally recognized. However, the concept of exerting metal ionic cue in bone TME without compromising the mechanical properties of bone allograft is challenging. To address this concern, an interfacial tissue microenvironment with magnesium cationc cue was tailored onto the gamma-irradiated allograft bone using a customized magnesium-plasma surface treatment. The formation of the Mg cationic cue enriched interfacial tissue microenvironment on allograft bone was verified by the scanning ion-selective electrode technique. The cellular activities of human TERT-immortalized mesenchymal stem cells on the Mg-enriched grafts were notably upregulated. In the animal test, superior osseointegration between Mg-enriched graft and host bone was found, whereas poor integration was observed in the gamma-irradiated controls at 28 days post-operation. Furthermore, the bony in-growth appeared on magnesium-enriched allograft bone was significant higher. The mechanism possibly correlates to the up-regulation of integrin receptors in mesenchymal stem cells under modified bone TME that directly orchestrate the initial cell attachment and osteogenic differentiation of mesenchymal stem cells. Lastly, our findings demonstrate the significance of magnesium cation modified bone allograft that can potentially translate to various orthopaedic procedures requiring bone augmentation.
format	article
author	Wenhao Wang Jie Shen Yuan Meng Miaoman Ye Shaozhang Lin Qi Zhao Le Wang Kenneth M.C. Cheung Shuilin Wu Yufeng Zheng Xuanyong Liu Paul K. Chu Kelvin W.K. Yeung Zhi-Yong Zhang
author_facet	Wenhao Wang Jie Shen Yuan Meng Miaoman Ye Shaozhang Lin Qi Zhao Le Wang Kenneth M.C. Cheung Shuilin Wu Yufeng Zheng Xuanyong Liu Paul K. Chu Kelvin W.K. Yeung Zhi-Yong Zhang
author_sort	Wenhao Wang
title	Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
title_short	Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
title_full	Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
title_fullStr	Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
title_full_unstemmed	Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
title_sort	magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/d3b388df26b84375a019dc73a2d5cf3c
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Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone

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