Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling

Abstract Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neur...

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Autores principales:	Juliette Bedrossiantz, Inmaculada Fuertes, Demetrio Raldua, Carlos Barata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3b48fea3fd242579ef577b42cd1538b
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spelling	oai:doaj.org-article:d3b48fea3fd242579ef577b42cd1538b2021-12-02T18:51:07ZPharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling10.1038/s41598-021-98886-w2045-2322https://doaj.org/article/d3b48fea3fd242579ef577b42cd1538b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98886-whttps://doaj.org/toc/2045-2322Abstract Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neurodevelopmental or neurofunctional mode of action. Phototactic behavioural changes against fish in the freshwater crustacean Daphnia magna have been the subject of many ecological investigations. The aim of this study was to identify which neurotransmitter systems modulate phototactic behaviour to fish kairomones. We used a positive phototactic D. magna clone (P132,85) that shows marked negative phototactism after exposure to fish kairomones. Treatments included up to 16 known agonists and antagonists of the serotonergic, cholinergic, dopaminergic, histaminergic, glutamatergic and GABAergic systems. It was hypothesized that many neurological signalling pathways may modulate D. magna phototactic behaviour to fish kairomones. A new custom-designed device with vertically oriented chambers was used, and changes in the preferred areas (bottom, middle, and upper areas) were analysed using groups of animals after 24 h of exposure to the selected substance(s). The results indicated that agonists of the muscarinic acetylcholine and GABAA receptors and their equi-effective mixture ameliorated the negative phototactic response to fish kairomones, whereas antagonists and their mixtures increased the negative phototactism to fish kairomones. Interestingly, inhibition of the muscarinic acetylcholine receptor abolished positive phototaxis, thus inducing the phototactic response to fish kairomones. Analysis of the profile of neurotransmitters and their related metabolites showed that the D. magna behavioural responses induced by fish depend on changes in the levels of acetylcholine, dopamine and GABA.Juliette BedrossiantzInmaculada FuertesDemetrio RalduaCarlos BarataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juliette Bedrossiantz Inmaculada Fuertes Demetrio Raldua Carlos Barata Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
description	Abstract Animal behaviour is closely related to individual fitness, which allows animals to choose suitable mates or avoid predation. The central nervous system regulates many aspects of animal behaviour responses. Therefore, behavioural responses can be especially sensitive to compounds with a neurodevelopmental or neurofunctional mode of action. Phototactic behavioural changes against fish in the freshwater crustacean Daphnia magna have been the subject of many ecological investigations. The aim of this study was to identify which neurotransmitter systems modulate phototactic behaviour to fish kairomones. We used a positive phototactic D. magna clone (P132,85) that shows marked negative phototactism after exposure to fish kairomones. Treatments included up to 16 known agonists and antagonists of the serotonergic, cholinergic, dopaminergic, histaminergic, glutamatergic and GABAergic systems. It was hypothesized that many neurological signalling pathways may modulate D. magna phototactic behaviour to fish kairomones. A new custom-designed device with vertically oriented chambers was used, and changes in the preferred areas (bottom, middle, and upper areas) were analysed using groups of animals after 24 h of exposure to the selected substance(s). The results indicated that agonists of the muscarinic acetylcholine and GABAA receptors and their equi-effective mixture ameliorated the negative phototactic response to fish kairomones, whereas antagonists and their mixtures increased the negative phototactism to fish kairomones. Interestingly, inhibition of the muscarinic acetylcholine receptor abolished positive phototaxis, thus inducing the phototactic response to fish kairomones. Analysis of the profile of neurotransmitters and their related metabolites showed that the D. magna behavioural responses induced by fish depend on changes in the levels of acetylcholine, dopamine and GABA.
format	article
author	Juliette Bedrossiantz Inmaculada Fuertes Demetrio Raldua Carlos Barata
author_facet	Juliette Bedrossiantz Inmaculada Fuertes Demetrio Raldua Carlos Barata
author_sort	Juliette Bedrossiantz
title	Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
title_short	Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
title_full	Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
title_fullStr	Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
title_full_unstemmed	Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling
title_sort	pharmacological modulation of fish-induced depth selection in d. magna: the role of cholinergic and gabaergic signalling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d3b48fea3fd242579ef577b42cd1538b
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_version_	1718377472978845696

Pharmacological modulation of fish-induced depth selection in D. magna: the role of cholinergic and GABAergic signalling

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