Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge

Abstract Background Treatment of wheat with the phytohormones abscisic acid (ABA) and gibberellic acid (GA) has been shown to affect Fusarium head blight (FHB) disease severity. However, the molecular mechanisms underlying the elicited phenotypes remain unclear. Toward addressing this gap in our kno...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leann M. Buhrow, Ziying Liu, Dustin Cram, Tanya Sharma, Nora A. Foroud, Youlian Pan, Michele C. Loewen
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Wheat Triticum aestivum Fusarium graminearum Fusarium head blight Phytohormone Abscisic acid Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d3b741ba85d9437eb9db7e26824c73ee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3b741ba85d9437eb9db7e26824c73ee
record_format	dspace
spelling	oai:doaj.org-article:d3b741ba85d9437eb9db7e26824c73ee2021-11-08T10:57:37ZWheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge10.1186/s12864-021-08069-01471-2164https://doaj.org/article/d3b741ba85d9437eb9db7e26824c73ee2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08069-0https://doaj.org/toc/1471-2164Abstract Background Treatment of wheat with the phytohormones abscisic acid (ABA) and gibberellic acid (GA) has been shown to affect Fusarium head blight (FHB) disease severity. However, the molecular mechanisms underlying the elicited phenotypes remain unclear. Toward addressing this gap in our knowledge, global transcriptomic profiling was applied to the FHB-susceptible wheat cultivar ‘Fielder’ to map the regulatory responses effected upon treatment with ABA, an ABA receptor antagonist (AS6), or GA in the presence or absence of Fusarium graminearum (Fg) challenge. Results Spike treatments resulted in a total of 30,876 differentially expressed genes (DEGs) identified in ‘Fielder’ (26,004) and the Fg (4872) pathogen. Topology overlap and correlation analyses defined 9689 wheat DEGs as Fg-related across the treatments. Further enrichment analyses demonstrated that these included expression changes within ‘Fielder’ defense responses, cell structural metabolism, molecular transport, and membrane/lipid metabolism. Dysregulation of ABA and GA crosstalk arising from repression of ‘Fielder’ FUS3 was noted. As well, expression of a putative Fg ABA-biosynthetic cytochrome P450 was detected. The co-applied condition of Fg + ABA elicited further up-regulation of phytohormone biosynthesis, as well as SA and ET signaling pathways and cell wall/polyphenolic metabolism. In contrast, co-applied Fg + GA mainly suppressed phytohormone biosynthesis and signaling, while modulating primary and secondary metabolism and flowering. Unexpectedly, co-applied Fg + AS6 did not affect ABA biosynthesis or signaling, but rather elicited antagonistic responses tied to stress, phytohormone transport, and FHB disease-related genes. Conclusions Observed exacerbation (misregulation) of classical defense mechanisms and cell wall fortifications upon ABA treatment are consistent with its ability to promote FHB severity and its proposed role as a fungal effector. In contrast, GA was found to modulate primary and secondary metabolism, suggesting a general metabolic shift underlying its reduction in FHB severity. While AS6 did not antagonize traditional ABA pathways, its impact on host defense and Fg responses imply potential for future investigation. Overall, by comparing these findings to those previously reported for four additional plant genotypes, an additive model of the wheat-Fg interaction is proposed in the context of phytohormone responses.Leann M. BuhrowZiying LiuDustin CramTanya SharmaNora A. ForoudYoulian PanMichele C. LoewenBMCarticleWheatTriticum aestivumFusarium graminearumFusarium head blightPhytohormoneAbscisic acidBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Wheat Triticum aestivum Fusarium graminearum Fusarium head blight Phytohormone Abscisic acid Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Wheat Triticum aestivum Fusarium graminearum Fusarium head blight Phytohormone Abscisic acid Biotechnology TP248.13-248.65 Genetics QH426-470 Leann M. Buhrow Ziying Liu Dustin Cram Tanya Sharma Nora A. Foroud Youlian Pan Michele C. Loewen Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
description	Abstract Background Treatment of wheat with the phytohormones abscisic acid (ABA) and gibberellic acid (GA) has been shown to affect Fusarium head blight (FHB) disease severity. However, the molecular mechanisms underlying the elicited phenotypes remain unclear. Toward addressing this gap in our knowledge, global transcriptomic profiling was applied to the FHB-susceptible wheat cultivar ‘Fielder’ to map the regulatory responses effected upon treatment with ABA, an ABA receptor antagonist (AS6), or GA in the presence or absence of Fusarium graminearum (Fg) challenge. Results Spike treatments resulted in a total of 30,876 differentially expressed genes (DEGs) identified in ‘Fielder’ (26,004) and the Fg (4872) pathogen. Topology overlap and correlation analyses defined 9689 wheat DEGs as Fg-related across the treatments. Further enrichment analyses demonstrated that these included expression changes within ‘Fielder’ defense responses, cell structural metabolism, molecular transport, and membrane/lipid metabolism. Dysregulation of ABA and GA crosstalk arising from repression of ‘Fielder’ FUS3 was noted. As well, expression of a putative Fg ABA-biosynthetic cytochrome P450 was detected. The co-applied condition of Fg + ABA elicited further up-regulation of phytohormone biosynthesis, as well as SA and ET signaling pathways and cell wall/polyphenolic metabolism. In contrast, co-applied Fg + GA mainly suppressed phytohormone biosynthesis and signaling, while modulating primary and secondary metabolism and flowering. Unexpectedly, co-applied Fg + AS6 did not affect ABA biosynthesis or signaling, but rather elicited antagonistic responses tied to stress, phytohormone transport, and FHB disease-related genes. Conclusions Observed exacerbation (misregulation) of classical defense mechanisms and cell wall fortifications upon ABA treatment are consistent with its ability to promote FHB severity and its proposed role as a fungal effector. In contrast, GA was found to modulate primary and secondary metabolism, suggesting a general metabolic shift underlying its reduction in FHB severity. While AS6 did not antagonize traditional ABA pathways, its impact on host defense and Fg responses imply potential for future investigation. Overall, by comparing these findings to those previously reported for four additional plant genotypes, an additive model of the wheat-Fg interaction is proposed in the context of phytohormone responses.
format	article
author	Leann M. Buhrow Ziying Liu Dustin Cram Tanya Sharma Nora A. Foroud Youlian Pan Michele C. Loewen
author_facet	Leann M. Buhrow Ziying Liu Dustin Cram Tanya Sharma Nora A. Foroud Youlian Pan Michele C. Loewen
author_sort	Leann M. Buhrow
title	Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
title_short	Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
title_full	Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
title_fullStr	Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
title_full_unstemmed	Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge
title_sort	wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following fusarium graminearum-challenge
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d3b741ba85d9437eb9db7e26824c73ee
work_keys_str_mv	AT leannmbuhrow wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT ziyingliu wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT dustincram wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT tanyasharma wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT noraaforoud wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT youlianpan wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge AT michelecloewen wheattranscriptomeprofilingrevealsabscisicandgibberellicacidtreatmentsregulateearlystagephytohormonedefensesignalingcellwallfortificationandmetabolicswitchesfollowingfusariumgraminearumchallenge
_version_	1718442473140781056

Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge

Ejemplares similares