Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia

Lakech Tibebu,1 Yerega Belete,2 Eyasu Tigabu,3 Wondewossen Tsegaye2 1Disease Surveillance Expert Epidemiology Directorate, Ministry of Agriculture, Addis Ababa, Ethiopia; 2Department of Microbiology, Immunology and Parasitology, St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia;...

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Autores principales:	Tibebu L, Belete Y, Tigabu E, Tsegaye W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	s. aureus mrsa mdr animal human interface Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d3bdc183557849d0bbe27f238b796385
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spelling	oai:doaj.org-article:d3bdc183557849d0bbe27f238b7963852021-12-02T18:47:16ZPrevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia2230-2034https://doaj.org/article/d3bdc183557849d0bbe27f238b7963852021-09-01T00:00:00Zhttps://www.dovepress.com/prevalence-of-staphylococcus-aureus-methicillin-resistant-staphylococc-peer-reviewed-fulltext-article-VMRRhttps://doaj.org/toc/2230-2034Lakech Tibebu,1 Yerega Belete,2 Eyasu Tigabu,3 Wondewossen Tsegaye2 1Disease Surveillance Expert Epidemiology Directorate, Ministry of Agriculture, Addis Ababa, Ethiopia; 2Department of Microbiology, Immunology and Parasitology, St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia; 3Research Directorate, Ethiopian Public Health Institute, Addis Ababa, EthiopiaCorrespondence: Yerega BeleteDepartment of Microbiology, Immunology and Parasitology, St. Paul’s Hospital Millennium Medical College, P.O. Box 1271, Addis Ababa, EthiopiaTel +251 920735677Email yereb2000@gmail.comBackground: Staphylococcus aureus (S. aureus) has been reported as the most commonly isolated highly contagious pathogen from human, animals and animal products. Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a significant pathogen with zoonotic potential that could have devastating consequence for the health and well-being of animals and human.Methods: A cross-sectional study was conducted from July 2020 to January 2021. A total of 233 samples from cow milk, udder swabs and milkers’ hand swabs were collected for culture and identification based on the standard protocol. Antimicrobial susceptibility tests were performed for all isolates by using Kirby Bauer’s disk diffusion test. MRSA was detected by cefoxitin disk diffusion test.Results: S. aureus was isolated from 50 (21.46%) of 233 samples and the prevalence of MRSA was 4%. The highest prevalence was found in cow milk 36 (25.53%) followed by hand swabs 10 (19.23%) and udder swabs 4 (10%). S. aureus prevalence was 58.33%, 30.0%, 21.43%, 17.92%, 15.79% in farm D, C, E, A, B respectively. A large percentage (58.33% and 30%) were from farm D and C. S. aureus isolation rate showed statistically significant association with farm types (p = 0.011) and with previous mastitis exposure (p = 0.001). High level of resistance was observed to penicillin (94%) and ampicillin (92%), but low level resistance to gentamicin (0%), amikacin (0%), ceftriaxone (0%), chloramphenicol (4%), ciprofloxacin and cefoxitin (4%). The overall prevalence of multidrug resistance (MDR) was 10.42%.Conclusion: Prevalence of S. aureus in milk showed statistically significant association with respect to previous mastitis exposure and farm types (p = 0.011). High level of resistant to penicillin and ampicillin was observed. Therefore, effective mastitis control programs, best veterinary practice among all farms and use of antibiotics in the farm should be strictly controlled.Keywords: S. aureus, MRSA, MDR, animal, human, interface, multidrug resistance, methicillin-resistance staphylococcus aureus, Staphylococcus aureusTibebu LBelete YTigabu ETsegaye WDove Medical Pressarticles. aureusmrsamdranimalhumaninterfaceVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol Volume 12, Pp 241-251 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	s. aureus mrsa mdr animal human interface Veterinary medicine SF600-1100
spellingShingle	s. aureus mrsa mdr animal human interface Veterinary medicine SF600-1100 Tibebu L Belete Y Tigabu E Tsegaye W Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
description	Lakech Tibebu,1 Yerega Belete,2 Eyasu Tigabu,3 Wondewossen Tsegaye2 1Disease Surveillance Expert Epidemiology Directorate, Ministry of Agriculture, Addis Ababa, Ethiopia; 2Department of Microbiology, Immunology and Parasitology, St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia; 3Research Directorate, Ethiopian Public Health Institute, Addis Ababa, EthiopiaCorrespondence: Yerega BeleteDepartment of Microbiology, Immunology and Parasitology, St. Paul’s Hospital Millennium Medical College, P.O. Box 1271, Addis Ababa, EthiopiaTel +251 920735677Email yereb2000@gmail.comBackground: Staphylococcus aureus (S. aureus) has been reported as the most commonly isolated highly contagious pathogen from human, animals and animal products. Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a significant pathogen with zoonotic potential that could have devastating consequence for the health and well-being of animals and human.Methods: A cross-sectional study was conducted from July 2020 to January 2021. A total of 233 samples from cow milk, udder swabs and milkers’ hand swabs were collected for culture and identification based on the standard protocol. Antimicrobial susceptibility tests were performed for all isolates by using Kirby Bauer’s disk diffusion test. MRSA was detected by cefoxitin disk diffusion test.Results: S. aureus was isolated from 50 (21.46%) of 233 samples and the prevalence of MRSA was 4%. The highest prevalence was found in cow milk 36 (25.53%) followed by hand swabs 10 (19.23%) and udder swabs 4 (10%). S. aureus prevalence was 58.33%, 30.0%, 21.43%, 17.92%, 15.79% in farm D, C, E, A, B respectively. A large percentage (58.33% and 30%) were from farm D and C. S. aureus isolation rate showed statistically significant association with farm types (p = 0.011) and with previous mastitis exposure (p = 0.001). High level of resistance was observed to penicillin (94%) and ampicillin (92%), but low level resistance to gentamicin (0%), amikacin (0%), ceftriaxone (0%), chloramphenicol (4%), ciprofloxacin and cefoxitin (4%). The overall prevalence of multidrug resistance (MDR) was 10.42%.Conclusion: Prevalence of S. aureus in milk showed statistically significant association with respect to previous mastitis exposure and farm types (p = 0.011). High level of resistant to penicillin and ampicillin was observed. Therefore, effective mastitis control programs, best veterinary practice among all farms and use of antibiotics in the farm should be strictly controlled.Keywords: S. aureus, MRSA, MDR, animal, human, interface, multidrug resistance, methicillin-resistance staphylococcus aureus, Staphylococcus aureus
format	article
author	Tibebu L Belete Y Tigabu E Tsegaye W
author_facet	Tibebu L Belete Y Tigabu E Tsegaye W
author_sort	Tibebu L
title	Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
title_short	Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
title_full	Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
title_fullStr	Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
title_full_unstemmed	Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia
title_sort	prevalence of staphylococcus aureus, methicillin-resistant staphylococcus aureus and potential risk factors in selected dairy farms at the interface of animal and human in bishoftu, ethiopia
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/d3bdc183557849d0bbe27f238b796385
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Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus and Potential Risk Factors in Selected Dairy Farms at the Interface of Animal and Human in Bishoftu, Ethiopia

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