Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)

The aim of this study was to characterize protease soluble collagen (PSC) obtained from milkfish scales, extraction using protease from proteolytic bacteria HTcUM7.1 isolate. The characterization included Fourier Transform Infra Red (FT-IR) spectra, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrop...

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Autores principales:	Nia Lutfiana Lutfiana, Suharti Suharti, Evi Susanti
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2019
Materias:	protease collagen soluble milkfish fish scales chanos chanos Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d3bfc7f3c5a446e298d2071e0c8b027e
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spelling	oai:doaj.org-article:d3bfc7f3c5a446e298d2071e0c8b027e2021-12-02T18:04:55ZCharacterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)2302-46902302-4690https://doaj.org/article/d3bfc7f3c5a446e298d2071e0c8b027e2019-12-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/506/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690The aim of this study was to characterize protease soluble collagen (PSC) obtained from milkfish scales, extraction using protease from proteolytic bacteria HTcUM7.1 isolate. The characterization included Fourier Transform Infra Red (FT-IR) spectra, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile, Field Emission Scanning Electron Microscopy (FESEM), denaturation temperature by Differential Scanning Calorimetric (DSC) and solubility. The resulting PSC from milkfish scales has white color, fiber with a length of about 20-60 µm, FTIR spectra and SDS-PAGE profile showed that PSC was collagen Type I and denaturation temperature was 145.48 °C, with maximum solubility at pH 1-3 and 1-2 % NaCl. Its high denaturation temperature value allows the collagen to be applied in the fields of medicines and cosmetics.Nia Lutfiana LutfianaSuharti SuhartiEvi SusantiUniversity of Brawijayaarticleprotease collagen solublemilkfish fishscaleschanos chanosChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 8, Iss 3, Pp 245-254 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	protease collagen soluble milkfish fish scales chanos chanos Chemistry QD1-999
spellingShingle	protease collagen soluble milkfish fish scales chanos chanos Chemistry QD1-999 Nia Lutfiana Lutfiana Suharti Suharti Evi Susanti Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
description	The aim of this study was to characterize protease soluble collagen (PSC) obtained from milkfish scales, extraction using protease from proteolytic bacteria HTcUM7.1 isolate. The characterization included Fourier Transform Infra Red (FT-IR) spectra, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile, Field Emission Scanning Electron Microscopy (FESEM), denaturation temperature by Differential Scanning Calorimetric (DSC) and solubility. The resulting PSC from milkfish scales has white color, fiber with a length of about 20-60 µm, FTIR spectra and SDS-PAGE profile showed that PSC was collagen Type I and denaturation temperature was 145.48 °C, with maximum solubility at pH 1-3 and 1-2 % NaCl. Its high denaturation temperature value allows the collagen to be applied in the fields of medicines and cosmetics.
format	article
author	Nia Lutfiana Lutfiana Suharti Suharti Evi Susanti
author_facet	Nia Lutfiana Lutfiana Suharti Suharti Evi Susanti
author_sort	Nia Lutfiana Lutfiana
title	Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
title_short	Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
title_full	Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
title_fullStr	Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
title_full_unstemmed	Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)
title_sort	characterization of protease soluble collagen (psc) from milkfish scales (chanos chanos)
publisher	University of Brawijaya
publishDate	2019
url	https://doaj.org/article/d3bfc7f3c5a446e298d2071e0c8b027e
work_keys_str_mv	AT nialutfianalutfiana characterizationofproteasesolublecollagenpscfrommilkfishscaleschanoschanos AT suhartisuharti characterizationofproteasesolublecollagenpscfrommilkfishscaleschanoschanos AT evisusanti characterizationofproteasesolublecollagenpscfrommilkfishscaleschanoschanos
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Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos)

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