AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 bind...

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Autores principales:	Marscha Hirschi, Wang-Ting Lu, Andrew Santiago-Frangos, Royce Wilkinson, Sarah M. Golden, Alan R. Davidson, Gabriel C. Lander, Blake Wiedenheft
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d3c21875e57643ea9c2043b059ad21af
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spelling	oai:doaj.org-article:d3c21875e57643ea9c2043b059ad21af2021-12-02T17:51:11ZAcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex10.1038/s41467-020-16512-12041-1723https://doaj.org/article/d3c21875e57643ea9c2043b059ad21af2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16512-1https://doaj.org/toc/2041-1723The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA.Marscha HirschiWang-Ting LuAndrew Santiago-FrangosRoyce WilkinsonSarah M. GoldenAlan R. DavidsonGabriel C. LanderBlake WiedenheftNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
description	The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA.
format	article
author	Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft
author_facet	Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft
author_sort	Marscha Hirschi
title	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_short	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_full	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_fullStr	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_full_unstemmed	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
title_sort	acrif9 tethers non-sequence specific dsdna to the crispr rna-guided surveillance complex
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d3c21875e57643ea9c2043b059ad21af
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AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

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