Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions

Measuring growth factors in single cells at physiologically relevant stimulation doses is challenging. Here the authors use fluorescent quantum dots and calibrated three-dimensional deconvolution microscopy to digitally count growth factors in single cells and reveal stimulation distributions in can...

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Autores principales: Phuong Le, Sung Jun Lim, Brian C. Baculis, Hee Jung Chung, Kristopher A. Kilian, Andrew M. Smith
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/d3c5be8c2a3e4caaac6ba0105ccb9d5c
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Sumario:Measuring growth factors in single cells at physiologically relevant stimulation doses is challenging. Here the authors use fluorescent quantum dots and calibrated three-dimensional deconvolution microscopy to digitally count growth factors in single cells and reveal stimulation distributions in cancer cells.