Genetic modification of primary human B cells to model high-grade lymphoma

A dearth of adequate preclinical models to faithfully mimic diffuse large B-cell lymphoma has hampered the identification of driver genes. Here, the authors present a co-culture system that enables ex vivo expansion, viral transduction and transformation of primary human germinal center B cells.

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Autores principales:	Rebecca Caeser, Miriam Di Re, Joanna A. Krupka, Jie Gao, Maribel Lara-Chica, João M. L. Dias, Susanna L. Cooke, Rachel Fenner, Zelvera Usheva, Hendrik F. P. Runge, Philip A. Beer, Hesham Eldaly, Hyo-Kyung Pak, Chan-Sik Park, George S. Vassiliou, Brian J. P. Huntly, Annalisa Mupo, Rachael J. M. Bashford-Rogers, Daniel J. Hodson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d3c9bc11a5cc4cb39c1c847ddd0e75b2
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spelling	oai:doaj.org-article:d3c9bc11a5cc4cb39c1c847ddd0e75b22021-12-02T15:35:27ZGenetic modification of primary human B cells to model high-grade lymphoma10.1038/s41467-019-12494-x2041-1723https://doaj.org/article/d3c9bc11a5cc4cb39c1c847ddd0e75b22019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12494-xhttps://doaj.org/toc/2041-1723A dearth of adequate preclinical models to faithfully mimic diffuse large B-cell lymphoma has hampered the identification of driver genes. Here, the authors present a co-culture system that enables ex vivo expansion, viral transduction and transformation of primary human germinal center B cells.Rebecca CaeserMiriam Di ReJoanna A. KrupkaJie GaoMaribel Lara-ChicaJoão M. L. DiasSusanna L. CookeRachel FennerZelvera UshevaHendrik F. P. RungePhilip A. BeerHesham EldalyHyo-Kyung PakChan-Sik ParkGeorge S. VassiliouBrian J. P. HuntlyAnnalisa MupoRachael J. M. Bashford-RogersDaniel J. HodsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Rebecca Caeser Miriam Di Re Joanna A. Krupka Jie Gao Maribel Lara-Chica João M. L. Dias Susanna L. Cooke Rachel Fenner Zelvera Usheva Hendrik F. P. Runge Philip A. Beer Hesham Eldaly Hyo-Kyung Pak Chan-Sik Park George S. Vassiliou Brian J. P. Huntly Annalisa Mupo Rachael J. M. Bashford-Rogers Daniel J. Hodson Genetic modification of primary human B cells to model high-grade lymphoma
description	A dearth of adequate preclinical models to faithfully mimic diffuse large B-cell lymphoma has hampered the identification of driver genes. Here, the authors present a co-culture system that enables ex vivo expansion, viral transduction and transformation of primary human germinal center B cells.
format	article
author	Rebecca Caeser Miriam Di Re Joanna A. Krupka Jie Gao Maribel Lara-Chica João M. L. Dias Susanna L. Cooke Rachel Fenner Zelvera Usheva Hendrik F. P. Runge Philip A. Beer Hesham Eldaly Hyo-Kyung Pak Chan-Sik Park George S. Vassiliou Brian J. P. Huntly Annalisa Mupo Rachael J. M. Bashford-Rogers Daniel J. Hodson
author_facet	Rebecca Caeser Miriam Di Re Joanna A. Krupka Jie Gao Maribel Lara-Chica João M. L. Dias Susanna L. Cooke Rachel Fenner Zelvera Usheva Hendrik F. P. Runge Philip A. Beer Hesham Eldaly Hyo-Kyung Pak Chan-Sik Park George S. Vassiliou Brian J. P. Huntly Annalisa Mupo Rachael J. M. Bashford-Rogers Daniel J. Hodson
author_sort	Rebecca Caeser
title	Genetic modification of primary human B cells to model high-grade lymphoma
title_short	Genetic modification of primary human B cells to model high-grade lymphoma
title_full	Genetic modification of primary human B cells to model high-grade lymphoma
title_fullStr	Genetic modification of primary human B cells to model high-grade lymphoma
title_full_unstemmed	Genetic modification of primary human B cells to model high-grade lymphoma
title_sort	genetic modification of primary human b cells to model high-grade lymphoma
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d3c9bc11a5cc4cb39c1c847ddd0e75b2
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Genetic modification of primary human B cells to model high-grade lymphoma

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