Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.

Understanding the early events during amyloid aggregation processes is crucial to single out the involved molecular mechanisms and for designing ad hoc strategies to prevent and reverse amyloidogenic disorders. Here, we show that, in conditions in which the protein is positively charged and its conf...

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Autores principales:	Valeria Vetri, Maurizio Leone, Ludmilla A Morozova-Roche, Bente Vestergaard, Vito Foderà
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3d003ff8fde47428e19f17525147bf5
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spelling	oai:doaj.org-article:d3d003ff8fde47428e19f17525147bf52021-11-18T07:37:30ZUnlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.1932-620310.1371/journal.pone.0068912https://doaj.org/article/d3d003ff8fde47428e19f17525147bf52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874809/?tool=EBIhttps://doaj.org/toc/1932-6203Understanding the early events during amyloid aggregation processes is crucial to single out the involved molecular mechanisms and for designing ad hoc strategies to prevent and reverse amyloidogenic disorders. Here, we show that, in conditions in which the protein is positively charged and its conformational flexibility is enhanced, Concanavalin A leads to fibril formation via a non-conventional aggregation pathway. Using a combination of light scattering, circular dichroism, small angle X-ray scattering, intrinsic (Tryptophan) and extrinsic (ANS) fluorescence and confocal and 2-photon fluorescence microscopy we characterize the aggregation process as a function of the temperature. We highlight a multi-step pathway with the formation of an on-pathway long-lived intermediate and a subsequent coagulation of such "crinkled" precursors into amyloid-like fibrils. The process results in a temperature-dependent aggregation-coagulation pathway, with the late phase of coagulation determined by the interplay between hydrophobic and electrostatic forces. Our data provide evidence for the complex aggregation pathway for a protein with a highly flexible native conformation. We demonstrate the possibility to generate a long-lived intermediate whose proportion and occurrence are easily tunable by experimental parameters (i.e. temperature). As a consequence, in the case of aggregation processes developing through well-defined energy barriers, our results can open the way to new strategies to induce more stable in vitro on-pathway intermediate species through a minute change in the initial conformational flexibility of the protein. This will allow isolating and experimentally studying such transient species, often indicated as relevant in neurodegenerative diseases, both in terms of structural and cytotoxic properties.Valeria VetriMaurizio LeoneLudmilla A Morozova-RocheBente VestergaardVito FoderàPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68912 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria Vetri Maurizio Leone Ludmilla A Morozova-Roche Bente Vestergaard Vito Foderà Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
description	Understanding the early events during amyloid aggregation processes is crucial to single out the involved molecular mechanisms and for designing ad hoc strategies to prevent and reverse amyloidogenic disorders. Here, we show that, in conditions in which the protein is positively charged and its conformational flexibility is enhanced, Concanavalin A leads to fibril formation via a non-conventional aggregation pathway. Using a combination of light scattering, circular dichroism, small angle X-ray scattering, intrinsic (Tryptophan) and extrinsic (ANS) fluorescence and confocal and 2-photon fluorescence microscopy we characterize the aggregation process as a function of the temperature. We highlight a multi-step pathway with the formation of an on-pathway long-lived intermediate and a subsequent coagulation of such "crinkled" precursors into amyloid-like fibrils. The process results in a temperature-dependent aggregation-coagulation pathway, with the late phase of coagulation determined by the interplay between hydrophobic and electrostatic forces. Our data provide evidence for the complex aggregation pathway for a protein with a highly flexible native conformation. We demonstrate the possibility to generate a long-lived intermediate whose proportion and occurrence are easily tunable by experimental parameters (i.e. temperature). As a consequence, in the case of aggregation processes developing through well-defined energy barriers, our results can open the way to new strategies to induce more stable in vitro on-pathway intermediate species through a minute change in the initial conformational flexibility of the protein. This will allow isolating and experimentally studying such transient species, often indicated as relevant in neurodegenerative diseases, both in terms of structural and cytotoxic properties.
format	article
author	Valeria Vetri Maurizio Leone Ludmilla A Morozova-Roche Bente Vestergaard Vito Foderà
author_facet	Valeria Vetri Maurizio Leone Ludmilla A Morozova-Roche Bente Vestergaard Vito Foderà
author_sort	Valeria Vetri
title	Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
title_short	Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
title_full	Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
title_fullStr	Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
title_full_unstemmed	Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
title_sort	unlocked concanavalin a forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d3d003ff8fde47428e19f17525147bf5
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Unlocked concanavalin A forms amyloid-like fibrils from coagulation of long-lived "crinkled" intermediates.

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