Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.

Cannabis sativa is relatively recalcitrant to de novo regeneration, but several studies have reported shoot organogenesis or somatic embryogenesis from non-meristematic tissues. Most report infrequent regeneration rates from these tissues, but a landmark publication from 2010 achieved regeneration f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adrian S Monthony, Sean T Kyne, Christopher M Grainger, Andrew Maxwell P Jones
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3d3bb20210742fe8269062e6ae2bcca
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3d3bb20210742fe8269062e6ae2bcca
record_format	dspace
spelling	oai:doaj.org-article:d3d3bb20210742fe8269062e6ae2bcca2021-12-02T20:15:02ZRecalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.1932-620310.1371/journal.pone.0235525https://doaj.org/article/d3d3bb20210742fe8269062e6ae2bcca2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0235525https://doaj.org/toc/1932-6203Cannabis sativa is relatively recalcitrant to de novo regeneration, but several studies have reported shoot organogenesis or somatic embryogenesis from non-meristematic tissues. Most report infrequent regeneration rates from these tissues, but a landmark publication from 2010 achieved regeneration from leaf explants with a 96% response rate, producing an average of 12.3 shoots per explant in a single drug-type accession. Despite the importance regeneration plays in plant biotechnology and the renewed interest in this crop the aforementioned protocol has not been used in subsequent papers in the decade since it was published, raising concerns over its reproducibility. Here we attempted to replicate this important Cannabis regeneration study and expand the original scope of the study by testing it across 10 drug-type C. sativa genotypes to assess genotypic variation. In our study, callus was induced in all 10 genotypes but callus growth and appearance substantially differed among cultivars, with the most responsive genotype producing 6-fold more callus than the least responsive. The shoot induction medium failed to induce shoot organogenesis in any of the 10 cultivars tested, instead resulting in necrosis of the calli. The findings of this replication study raise concerns about the replicability of existing methods. However, some details of the protocol could not be replicated due to missing details in the original paper and regulatory issues, which could have impacted the outcome. These results highlight the importance of using multiple genotypes in such studies and providing detailed methods to facilitate replication.Adrian S MonthonySean T KyneChristopher M GraingerAndrew Maxwell P JonesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0235525 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrian S Monthony Sean T Kyne Christopher M Grainger Andrew Maxwell P Jones Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
description	Cannabis sativa is relatively recalcitrant to de novo regeneration, but several studies have reported shoot organogenesis or somatic embryogenesis from non-meristematic tissues. Most report infrequent regeneration rates from these tissues, but a landmark publication from 2010 achieved regeneration from leaf explants with a 96% response rate, producing an average of 12.3 shoots per explant in a single drug-type accession. Despite the importance regeneration plays in plant biotechnology and the renewed interest in this crop the aforementioned protocol has not been used in subsequent papers in the decade since it was published, raising concerns over its reproducibility. Here we attempted to replicate this important Cannabis regeneration study and expand the original scope of the study by testing it across 10 drug-type C. sativa genotypes to assess genotypic variation. In our study, callus was induced in all 10 genotypes but callus growth and appearance substantially differed among cultivars, with the most responsive genotype producing 6-fold more callus than the least responsive. The shoot induction medium failed to induce shoot organogenesis in any of the 10 cultivars tested, instead resulting in necrosis of the calli. The findings of this replication study raise concerns about the replicability of existing methods. However, some details of the protocol could not be replicated due to missing details in the original paper and regulatory issues, which could have impacted the outcome. These results highlight the importance of using multiple genotypes in such studies and providing detailed methods to facilitate replication.
format	article
author	Adrian S Monthony Sean T Kyne Christopher M Grainger Andrew Maxwell P Jones
author_facet	Adrian S Monthony Sean T Kyne Christopher M Grainger Andrew Maxwell P Jones
author_sort	Adrian S Monthony
title	Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
title_short	Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
title_full	Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
title_fullStr	Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
title_full_unstemmed	Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.
title_sort	recalcitrance of cannabis sativa to de novo regeneration; a multi-genotype replication study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d3d3bb20210742fe8269062e6ae2bcca
work_keys_str_mv	AT adriansmonthony recalcitranceofcannabissativatodenovoregenerationamultigenotypereplicationstudy AT seantkyne recalcitranceofcannabissativatodenovoregenerationamultigenotypereplicationstudy AT christophermgrainger recalcitranceofcannabissativatodenovoregenerationamultigenotypereplicationstudy AT andrewmaxwellpjones recalcitranceofcannabissativatodenovoregenerationamultigenotypereplicationstudy
_version_	1718374611714834432

Recalcitrance of Cannabis sativa to de novo regeneration; a multi-genotype replication study.

Ejemplares similares