RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli

Abstract High hydrostatic pressure (HHP) processing is an attractive non-thermal alternative to food pasteurization. Nevertheless, the large inter- and intra-species variations in HHP resistance among foodborne pathogens and the ease by which they can acquire extreme resistance are an issue of incre...

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Autores principales:	Elisa Gayán, Alexander Cambré, Chris W. Michiels, Abram Aertsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3d6b80ba5944f4a98747c197fbd8496
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spelling	oai:doaj.org-article:d3d6b80ba5944f4a98747c197fbd84962021-12-02T11:53:07ZRpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli10.1038/s41598-017-08958-z2045-2322https://doaj.org/article/d3d6b80ba5944f4a98747c197fbd84962017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08958-zhttps://doaj.org/toc/2045-2322Abstract High hydrostatic pressure (HHP) processing is an attractive non-thermal alternative to food pasteurization. Nevertheless, the large inter- and intra-species variations in HHP resistance among foodborne pathogens and the ease by which they can acquire extreme resistance are an issue of increasing concern. Since RpoS activity has been considered as a central determinant in the HHP resistance of E. coli and its pathovars, this study probed for the potential of an E. coli MG1655 ΔrpoS mutant to acquire HHP resistance by directed evolution. Despite the higher initial HHP sensitivity of the ΔrpoS mutant compared to the wild-type strain, evolved lineages of the former readily managed to restore or even succeed wild-type levels of resistance. A number of these ΔrpoS derivatives were affected in cAMP/CRP regulation, and this could be causally related to their HHP resistance. Subsequent inspection revealed that some of previously isolated HHP-resistant mutants derived from the wild-type strain also incurred a causal decrease in cAMP/CRP regulation. cAMP/CRP attenuated HHP-resistant mutants also exhibited higher resistance to fosfomycin, a preferred treatment for STEC infections. As such, this study reveals attenuation of cAMP/CRP regulation as a relevant and RpoS-independent evolutionary route towards HHP resistance in E. coli that coincides with fosfomycin resistance.Elisa GayánAlexander CambréChris W. MichielsAbram AertsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisa Gayán Alexander Cambré Chris W. Michiels Abram Aertsen RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
description	Abstract High hydrostatic pressure (HHP) processing is an attractive non-thermal alternative to food pasteurization. Nevertheless, the large inter- and intra-species variations in HHP resistance among foodborne pathogens and the ease by which they can acquire extreme resistance are an issue of increasing concern. Since RpoS activity has been considered as a central determinant in the HHP resistance of E. coli and its pathovars, this study probed for the potential of an E. coli MG1655 ΔrpoS mutant to acquire HHP resistance by directed evolution. Despite the higher initial HHP sensitivity of the ΔrpoS mutant compared to the wild-type strain, evolved lineages of the former readily managed to restore or even succeed wild-type levels of resistance. A number of these ΔrpoS derivatives were affected in cAMP/CRP regulation, and this could be causally related to their HHP resistance. Subsequent inspection revealed that some of previously isolated HHP-resistant mutants derived from the wild-type strain also incurred a causal decrease in cAMP/CRP regulation. cAMP/CRP attenuated HHP-resistant mutants also exhibited higher resistance to fosfomycin, a preferred treatment for STEC infections. As such, this study reveals attenuation of cAMP/CRP regulation as a relevant and RpoS-independent evolutionary route towards HHP resistance in E. coli that coincides with fosfomycin resistance.
format	article
author	Elisa Gayán Alexander Cambré Chris W. Michiels Abram Aertsen
author_facet	Elisa Gayán Alexander Cambré Chris W. Michiels Abram Aertsen
author_sort	Elisa Gayán
title	RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
title_short	RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
title_full	RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
title_fullStr	RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
title_full_unstemmed	RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli
title_sort	rpos-independent evolution reveals the importance of attenuated camp/crp regulation in high hydrostatic pressure resistance acquisition in e. coli
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d3d6b80ba5944f4a98747c197fbd8496
work_keys_str_mv	AT elisagayan rposindependentevolutionrevealstheimportanceofattenuatedcampcrpregulationinhighhydrostaticpressureresistanceacquisitioninecoli AT alexandercambre rposindependentevolutionrevealstheimportanceofattenuatedcampcrpregulationinhighhydrostaticpressureresistanceacquisitioninecoli AT chriswmichiels rposindependentevolutionrevealstheimportanceofattenuatedcampcrpregulationinhighhydrostaticpressureresistanceacquisitioninecoli AT abramaertsen rposindependentevolutionrevealstheimportanceofattenuatedcampcrpregulationinhighhydrostaticpressureresistanceacquisitioninecoli
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RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli

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