Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.

Swine influenza viruses (SIV) regularly cause significant disease in pigs worldwide. Since there is no causative treatment of SIV, we tested if probiotic Enterococcus (E.) faecium NCIMB 10415 or zinc (Zn) oxide as feed supplements provide beneficial effects upon SIV infection in piglets. Seventy-two...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhenya Wang, Michael Burwinkel, Weidong Chai, Elke Lange, Ulrike Blohm, Angele Breithaupt, Bernd Hoffmann, Sven Twardziok, Juliane Rieger, Pawel Janczyk, Robert Pieper, Nikolaus Osterrieder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3df84254de9481dbd1c4d4021ba3a5b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3df84254de9481dbd1c4d4021ba3a5b
record_format	dspace
spelling	oai:doaj.org-article:d3df84254de9481dbd1c4d4021ba3a5b2021-11-18T08:35:20ZDietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.1932-620310.1371/journal.pone.0087007https://doaj.org/article/d3df84254de9481dbd1c4d4021ba3a5b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24489827/?tool=EBIhttps://doaj.org/toc/1932-6203Swine influenza viruses (SIV) regularly cause significant disease in pigs worldwide. Since there is no causative treatment of SIV, we tested if probiotic Enterococcus (E.) faecium NCIMB 10415 or zinc (Zn) oxide as feed supplements provide beneficial effects upon SIV infection in piglets. Seventy-two weaned piglets were fed three different diets containing either E. faecium or different levels of Zn (2500 ppm, Zn(high); 50 ppm, Zn(low)). Half of the piglets were vaccinated intramuscularly (VAC) twice with an inactivated trivalent SIV vaccine, while all piglets were then infected intranasally with H3N2 SIV. Significantly higher weekly weight gains were observed in the E. faecium group before virus infection, and piglets in Zn(high) and E. faecium groups gained weight after infection while those in the control group (Zn(low)) lost weight. Using ELISA, we found significantly higher H3N2-specific antibody levels in the E. faecium+VAC group 2 days before and at the day of challenge infection as well as at 4 and 6 days after challenge infection. Higher hemagglutination inhibition (HI) titers were also observed in the Zn(high)+VAC and E. faecium+VAC groups at 0, 1 and 4 days after infection. However, there were no significant differences in virus shedding and lung lesions between the dietary groups. Using flow cytometry analysis significantly higher activated T helper cells and cytotoxic T lymphocyte percentages in the PBMCs were detected in the Zn(high) and E. faecium groups at single time points after infection compared to the Zn(low) control group, but no prolonged effect was found. In the BAL cells no influence of dietary supplementation on immune cell percentages could be detected. Our results suggest that feeding high doses of zinc oxide and particularly E. faecium could beneficially influence humoral immune responses after vaccination and recovery from SIV infection, but not affect virus shedding and lung pathology.Zhenya WangMichael BurwinkelWeidong ChaiElke LangeUlrike BlohmAngele BreithauptBernd HoffmannSven TwardziokJuliane RiegerPawel JanczykRobert PieperNikolaus OsterriederPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87007 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhenya Wang Michael Burwinkel Weidong Chai Elke Lange Ulrike Blohm Angele Breithaupt Bernd Hoffmann Sven Twardziok Juliane Rieger Pawel Janczyk Robert Pieper Nikolaus Osterrieder Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
description	Swine influenza viruses (SIV) regularly cause significant disease in pigs worldwide. Since there is no causative treatment of SIV, we tested if probiotic Enterococcus (E.) faecium NCIMB 10415 or zinc (Zn) oxide as feed supplements provide beneficial effects upon SIV infection in piglets. Seventy-two weaned piglets were fed three different diets containing either E. faecium or different levels of Zn (2500 ppm, Zn(high); 50 ppm, Zn(low)). Half of the piglets were vaccinated intramuscularly (VAC) twice with an inactivated trivalent SIV vaccine, while all piglets were then infected intranasally with H3N2 SIV. Significantly higher weekly weight gains were observed in the E. faecium group before virus infection, and piglets in Zn(high) and E. faecium groups gained weight after infection while those in the control group (Zn(low)) lost weight. Using ELISA, we found significantly higher H3N2-specific antibody levels in the E. faecium+VAC group 2 days before and at the day of challenge infection as well as at 4 and 6 days after challenge infection. Higher hemagglutination inhibition (HI) titers were also observed in the Zn(high)+VAC and E. faecium+VAC groups at 0, 1 and 4 days after infection. However, there were no significant differences in virus shedding and lung lesions between the dietary groups. Using flow cytometry analysis significantly higher activated T helper cells and cytotoxic T lymphocyte percentages in the PBMCs were detected in the Zn(high) and E. faecium groups at single time points after infection compared to the Zn(low) control group, but no prolonged effect was found. In the BAL cells no influence of dietary supplementation on immune cell percentages could be detected. Our results suggest that feeding high doses of zinc oxide and particularly E. faecium could beneficially influence humoral immune responses after vaccination and recovery from SIV infection, but not affect virus shedding and lung pathology.
format	article
author	Zhenya Wang Michael Burwinkel Weidong Chai Elke Lange Ulrike Blohm Angele Breithaupt Bernd Hoffmann Sven Twardziok Juliane Rieger Pawel Janczyk Robert Pieper Nikolaus Osterrieder
author_facet	Zhenya Wang Michael Burwinkel Weidong Chai Elke Lange Ulrike Blohm Angele Breithaupt Bernd Hoffmann Sven Twardziok Juliane Rieger Pawel Janczyk Robert Pieper Nikolaus Osterrieder
author_sort	Zhenya Wang
title	Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
title_short	Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
title_full	Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
title_fullStr	Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
title_full_unstemmed	Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
title_sort	dietary enterococcus faecium ncimb 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d3df84254de9481dbd1c4d4021ba3a5b
work_keys_str_mv	AT zhenyawang dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT michaelburwinkel dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT weidongchai dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT elkelange dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT ulrikeblohm dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT angelebreithaupt dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT berndhoffmann dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT sventwardziok dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT julianerieger dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT paweljanczyk dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT robertpieper dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection AT nikolausosterrieder dietaryenterococcusfaeciumncimb10415andzincoxidestimulateimmunereactionstotrivalentinfluenzavaccinationinpigsbutdonotaffectvirologicalresponseuponchallengeinfection
_version_	1718421590618669056

Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection.

Ejemplares similares