Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria

Abstract Antimicrobial peptides (AMPs) are components of immune defense in many organisms, including plants. They combat pathogens due to their antiviral, antifungal and antibacterial properties, and are considered potential therapeutic agents. An example of AMP is Epsilon-Poly-l-lysine (EPL), a pol...

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Autores principales:	Bárbara Rodrigues, Tâmara P. Morais, Paulo A. Zaini, Cássio S. Campos, Hebréia O. Almeida-Souza, Abhaya M. Dandekar, Rafael Nascimento, Luiz R. Goulart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3e4d47a2e7c4d7d9a309f1fd17f5d36
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spelling	oai:doaj.org-article:d3e4d47a2e7c4d7d9a309f1fd17f5d362021-12-02T16:24:49ZAntimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria10.1038/s41598-020-68262-12045-2322https://doaj.org/article/d3e4d47a2e7c4d7d9a309f1fd17f5d362020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68262-1https://doaj.org/toc/2045-2322Abstract Antimicrobial peptides (AMPs) are components of immune defense in many organisms, including plants. They combat pathogens due to their antiviral, antifungal and antibacterial properties, and are considered potential therapeutic agents. An example of AMP is Epsilon-Poly-l-lysine (EPL), a polypeptide formed by ~ 25 lysine residues with known antimicrobial activity against several human microbial pathogens. EPL presents some advantages such as good water solubility, thermal stability, biodegradability, and low toxicity, being a candidate for the control of phytopathogens. Our aim was to evaluate the antimicrobial activity of EPL against four phytobacterial species spanning different classes within the Gram-negative phylum Proteobacteria: Agrobacterium tumefaciens (syn. Rhizobium radiobacter), Ralstonia solanacearum, Xanthomonas citri subsp. citri (X. citri), and Xanthomonas euvesicatoria. The minimum inhibitory concentration (MIC) of the peptide ranged from 80 μg/ml for X. citri to 600 μg/ml for R. solanacearum and X. euvesicatoria. Two hours of MIC exposure led to pathogen death due to cell lysis and was enough for pathogen clearance. The protective and curative effects of EPL were demonstrated on tomato plants inoculated with X. euvesicatoria. Plants showed less disease severity when sprayed with EPL solution, making it a promising natural product for the control of plant diseases caused by diverse Proteobacteria.Bárbara RodriguesTâmara P. MoraisPaulo A. ZainiCássio S. CamposHebréia O. Almeida-SouzaAbhaya M. DandekarRafael NascimentoLuiz R. GoulartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bárbara Rodrigues Tâmara P. Morais Paulo A. Zaini Cássio S. Campos Hebréia O. Almeida-Souza Abhaya M. Dandekar Rafael Nascimento Luiz R. Goulart Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
description	Abstract Antimicrobial peptides (AMPs) are components of immune defense in many organisms, including plants. They combat pathogens due to their antiviral, antifungal and antibacterial properties, and are considered potential therapeutic agents. An example of AMP is Epsilon-Poly-l-lysine (EPL), a polypeptide formed by ~ 25 lysine residues with known antimicrobial activity against several human microbial pathogens. EPL presents some advantages such as good water solubility, thermal stability, biodegradability, and low toxicity, being a candidate for the control of phytopathogens. Our aim was to evaluate the antimicrobial activity of EPL against four phytobacterial species spanning different classes within the Gram-negative phylum Proteobacteria: Agrobacterium tumefaciens (syn. Rhizobium radiobacter), Ralstonia solanacearum, Xanthomonas citri subsp. citri (X. citri), and Xanthomonas euvesicatoria. The minimum inhibitory concentration (MIC) of the peptide ranged from 80 μg/ml for X. citri to 600 μg/ml for R. solanacearum and X. euvesicatoria. Two hours of MIC exposure led to pathogen death due to cell lysis and was enough for pathogen clearance. The protective and curative effects of EPL were demonstrated on tomato plants inoculated with X. euvesicatoria. Plants showed less disease severity when sprayed with EPL solution, making it a promising natural product for the control of plant diseases caused by diverse Proteobacteria.
format	article
author	Bárbara Rodrigues Tâmara P. Morais Paulo A. Zaini Cássio S. Campos Hebréia O. Almeida-Souza Abhaya M. Dandekar Rafael Nascimento Luiz R. Goulart
author_facet	Bárbara Rodrigues Tâmara P. Morais Paulo A. Zaini Cássio S. Campos Hebréia O. Almeida-Souza Abhaya M. Dandekar Rafael Nascimento Luiz R. Goulart
author_sort	Bárbara Rodrigues
title	Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
title_short	Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
title_full	Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
title_fullStr	Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
title_full_unstemmed	Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria
title_sort	antimicrobial activity of epsilon-poly-l-lysine against phytopathogenic bacteria
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d3e4d47a2e7c4d7d9a309f1fd17f5d36
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Antimicrobial activity of Epsilon-Poly-l-lysine against phytopathogenic bacteria

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