mRNA localization mechanisms in Trypanosoma cruzi.

Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lysangela R Alves, Eloise P Guerra-Slompo, Arthur V de Oliveira, Juliane S Malgarin, Samuel Goldenberg, Bruno Dallagiovanna
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3e52d0419ea47b09004348fb49f80d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3e52d0419ea47b09004348fb49f80d3
record_format	dspace
spelling	oai:doaj.org-article:d3e52d0419ea47b09004348fb49f80d32021-11-18T08:43:32ZmRNA localization mechanisms in Trypanosoma cruzi.1932-620310.1371/journal.pone.0081375https://doaj.org/article/d3e52d0419ea47b09004348fb49f80d32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324687/?tool=EBIhttps://doaj.org/toc/1932-6203Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient eukaryotes that branched off early in eukaryote evolution. We hypothesized that these organisms would have basic mechanisms of mRNA localization. FISH assays with probes against transcripts coding for proteins with restricted distributions showed a discrete localization of the mRNAs in the cytoplasm. Moreover, cruzipain mRNA was found inside reservosomes suggesting new unexpected functions for this vacuolar organelle. Individual mRNAs were also mobilized to RNA granules in response to nutritional stress. The cytoplasmic distribution of these transcripts changed with cell differentiation, suggesting that localization mechanisms might be involved in the regulation of stage-specific protein expression. Transfection assays with reporter genes showed that, as in higher eukaryotes, 3'UTRs were responsible for guiding mRNAs to their final location. Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. This kind of controlled mRNA transport is ancient, dating back to early eukaryote evolution.Lysangela R AlvesEloise P Guerra-SlompoArthur V de OliveiraJuliane S MalgarinSamuel GoldenbergBruno DallagiovannaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81375 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lysangela R Alves Eloise P Guerra-Slompo Arthur V de Oliveira Juliane S Malgarin Samuel Goldenberg Bruno Dallagiovanna mRNA localization mechanisms in Trypanosoma cruzi.
description	Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient eukaryotes that branched off early in eukaryote evolution. We hypothesized that these organisms would have basic mechanisms of mRNA localization. FISH assays with probes against transcripts coding for proteins with restricted distributions showed a discrete localization of the mRNAs in the cytoplasm. Moreover, cruzipain mRNA was found inside reservosomes suggesting new unexpected functions for this vacuolar organelle. Individual mRNAs were also mobilized to RNA granules in response to nutritional stress. The cytoplasmic distribution of these transcripts changed with cell differentiation, suggesting that localization mechanisms might be involved in the regulation of stage-specific protein expression. Transfection assays with reporter genes showed that, as in higher eukaryotes, 3'UTRs were responsible for guiding mRNAs to their final location. Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. This kind of controlled mRNA transport is ancient, dating back to early eukaryote evolution.
format	article
author	Lysangela R Alves Eloise P Guerra-Slompo Arthur V de Oliveira Juliane S Malgarin Samuel Goldenberg Bruno Dallagiovanna
author_facet	Lysangela R Alves Eloise P Guerra-Slompo Arthur V de Oliveira Juliane S Malgarin Samuel Goldenberg Bruno Dallagiovanna
author_sort	Lysangela R Alves
title	mRNA localization mechanisms in Trypanosoma cruzi.
title_short	mRNA localization mechanisms in Trypanosoma cruzi.
title_full	mRNA localization mechanisms in Trypanosoma cruzi.
title_fullStr	mRNA localization mechanisms in Trypanosoma cruzi.
title_full_unstemmed	mRNA localization mechanisms in Trypanosoma cruzi.
title_sort	mrna localization mechanisms in trypanosoma cruzi.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d3e52d0419ea47b09004348fb49f80d3
work_keys_str_mv	AT lysangelaralves mrnalocalizationmechanismsintrypanosomacruzi AT eloisepguerraslompo mrnalocalizationmechanismsintrypanosomacruzi AT arthurvdeoliveira mrnalocalizationmechanismsintrypanosomacruzi AT julianesmalgarin mrnalocalizationmechanismsintrypanosomacruzi AT samuelgoldenberg mrnalocalizationmechanismsintrypanosomacruzi AT brunodallagiovanna mrnalocalizationmechanismsintrypanosomacruzi
_version_	1718421411798712320

mRNA localization mechanisms in Trypanosoma cruzi.

Ejemplares similares