Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

Murali Mohan Yallapu1, Mara C Ebeling1, Neeraj Chauhan1,3, Meena Jaggi1-3, Subhash C Chauhan1-31Cancer Biology Research Center, Sanford Research, 2Department of Obstetrics and Gynecology, 3Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota, Sioux Falls, SDBackg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yallapu MM, Ebeling MC, Chauhan N, Jaggi M, Chauhan SC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d3e6df7959c548c89d950809179182b2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3e6df7959c548c89d950809179182b2
record_format	dspace
spelling	oai:doaj.org-article:d3e6df7959c548c89d950809179182b22021-12-02T01:13:55ZInteraction of curcumin nanoformulations with human plasma proteins and erythrocytes1176-91141178-2013https://doaj.org/article/d3e6df7959c548c89d950809179182b22011-11-01T00:00:00Zhttp://www.dovepress.com/interaction-of-curcumin-nanoformulations-with-human-plasma-proteins-an-a8611https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Murali Mohan Yallapu1, Mara C Ebeling1, Neeraj Chauhan1,3, Meena Jaggi1-3, Subhash C Chauhan1-31Cancer Biology Research Center, Sanford Research, 2Department of Obstetrics and Gynecology, 3Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota, Sioux Falls, SDBackground: Recent studies report curcumin nanoformulation(s) based on polylactic-co-glycolic acid (PLGA), &szlig;-cyclodextrin, cellulose, nanogel, and dendrimers to have anticancer potential. However, no comparative data are currently available for the interaction of curcumin nanoformulations with blood proteins and erythrocytes. The objective of this study was to examine the interaction of curcumin nanoformulations with cancer cells, serum proteins, and human red blood cells, and to assess their potential application for in vivo preclinical and clinical studies.Methods: The cellular uptake of curcumin nanoformulations was assessed by measuring curcumin levels in cancer cells using ultraviolet-visible spectrophotometry. Protein interaction studies were conducted using particle size analysis, zeta potential, and Western blot techniques. Curcumin nanoformulations were incubated with human red blood cells to evaluate their acute toxicity and hemocompatibility.Results: Cellular uptake of curcumin nanoformulations by cancer cells demonstrated preferential uptake versus free curcumin. Particle sizes and zeta potentials of curucumin nanoformulations were varied after human serum albumin adsorption. A remarkable capacity of the dendrimer curcumin nanoformulation to bind to plasma protein was observed, while the other formulations showed minimal binding capacity. Dendrimer curcumin nanoformulations also showed higher toxicity to red blood cells compared with the other curcumin nanoformulations.Conclusion: PLGA and nanogel curcumin nanoformulations appear to be very compatible with erythrocytes and have low serum protein binding characteristics, which suggests that they may be suitable for application in the treatment of malignancy. These findings advance our understanding of the characteristics of curcumin nanoformulations, a necessary component in harnessing and implementing improved in vivo effects of curcumin.Keywords: nanoparticle, curcumin, chemotherapy, cellular uptake, protein binding, hemocompatibilityYallapu MMEbeling MCChauhan NJaggi MChauhan SCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2779-2790 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yallapu MM Ebeling MC Chauhan N Jaggi M Chauhan SC Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
description	Murali Mohan Yallapu1, Mara C Ebeling1, Neeraj Chauhan1,3, Meena Jaggi1-3, Subhash C Chauhan1-31Cancer Biology Research Center, Sanford Research, 2Department of Obstetrics and Gynecology, 3Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota, Sioux Falls, SDBackground: Recent studies report curcumin nanoformulation(s) based on polylactic-co-glycolic acid (PLGA), &szlig;-cyclodextrin, cellulose, nanogel, and dendrimers to have anticancer potential. However, no comparative data are currently available for the interaction of curcumin nanoformulations with blood proteins and erythrocytes. The objective of this study was to examine the interaction of curcumin nanoformulations with cancer cells, serum proteins, and human red blood cells, and to assess their potential application for in vivo preclinical and clinical studies.Methods: The cellular uptake of curcumin nanoformulations was assessed by measuring curcumin levels in cancer cells using ultraviolet-visible spectrophotometry. Protein interaction studies were conducted using particle size analysis, zeta potential, and Western blot techniques. Curcumin nanoformulations were incubated with human red blood cells to evaluate their acute toxicity and hemocompatibility.Results: Cellular uptake of curcumin nanoformulations by cancer cells demonstrated preferential uptake versus free curcumin. Particle sizes and zeta potentials of curucumin nanoformulations were varied after human serum albumin adsorption. A remarkable capacity of the dendrimer curcumin nanoformulation to bind to plasma protein was observed, while the other formulations showed minimal binding capacity. Dendrimer curcumin nanoformulations also showed higher toxicity to red blood cells compared with the other curcumin nanoformulations.Conclusion: PLGA and nanogel curcumin nanoformulations appear to be very compatible with erythrocytes and have low serum protein binding characteristics, which suggests that they may be suitable for application in the treatment of malignancy. These findings advance our understanding of the characteristics of curcumin nanoformulations, a necessary component in harnessing and implementing improved in vivo effects of curcumin.Keywords: nanoparticle, curcumin, chemotherapy, cellular uptake, protein binding, hemocompatibility
format	article
author	Yallapu MM Ebeling MC Chauhan N Jaggi M Chauhan SC
author_facet	Yallapu MM Ebeling MC Chauhan N Jaggi M Chauhan SC
author_sort	Yallapu MM
title	Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
title_short	Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
title_full	Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
title_fullStr	Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
title_full_unstemmed	Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
title_sort	interaction of curcumin nanoformulations with human plasma proteins and erythrocytes
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/d3e6df7959c548c89d950809179182b2
work_keys_str_mv	AT yallapumm interactionofcurcuminnanoformulationswithhumanplasmaproteinsanderythrocytes AT ebelingmc interactionofcurcuminnanoformulationswithhumanplasmaproteinsanderythrocytes AT chauhann interactionofcurcuminnanoformulationswithhumanplasmaproteinsanderythrocytes AT jaggim interactionofcurcuminnanoformulationswithhumanplasmaproteinsanderythrocytes AT chauhansc interactionofcurcuminnanoformulationswithhumanplasmaproteinsanderythrocytes
_version_	1718403226390233088

Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

Ejemplares similares