Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi

Abstract Ixodes scapularis ticks transmit multiple pathogens, including Borrelia burgdorferi sensu stricto, and encode many proteins harboring epidermal growth factor (EGF)-like domains. We show that I. scapularis produces multiple orthologs for Bm86, a widely studied tick gut protein considered as...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juraj Koči, Sandhya Bista, Payal Chirania, Xiuli Yang, Chrysoula Kitsou, Vipin Singh Rana, Ozlem Buyuktanir Yas, Daniel E. Sonenshine, Utpal Pal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3ec3b13e732426eba0e7d71824fba0b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3ec3b13e732426eba0e7d71824fba0b
record_format	dspace
spelling	oai:doaj.org-article:d3ec3b13e732426eba0e7d71824fba0b2021-12-02T11:39:27ZAntibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi10.1038/s41598-021-85624-52045-2322https://doaj.org/article/d3ec3b13e732426eba0e7d71824fba0b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85624-5https://doaj.org/toc/2045-2322Abstract Ixodes scapularis ticks transmit multiple pathogens, including Borrelia burgdorferi sensu stricto, and encode many proteins harboring epidermal growth factor (EGF)-like domains. We show that I. scapularis produces multiple orthologs for Bm86, a widely studied tick gut protein considered as a target of an anti-tick vaccine, herein termed as Is86. We show that Is86 antigens feature at least three identifiable regions harboring EGF-like domains (termed as EGF-1, EGF-2, and EGF-3) and are differentially upregulated during B. burgdorferi infection. Although the RNA interference-mediated knockdown of Is86 genes did not show any influences on tick engorgement or B. burgdorferi sensu stricto persistence, the immunization of murine hosts with specific recombinant EGF antigens marginally reduced spirochete loads in the skin, in addition to affecting tick blood meal engorgement and molting. However, given the borderline impact of EGF immunization on tick engorgement and pathogen survival in the vector, it is unlikely that these antigens, at least in their current forms, could be developed as potential vaccines. Further investigations of the biological significance of Is86 (and other tick antigens) would enrich our knowledge of the intricate biology of ticks, including their interactions with resident pathogens, and contribute to the development of anti-tick measures to combat tick-borne illnesses.Juraj KočiSandhya BistaPayal ChiraniaXiuli YangChrysoula KitsouVipin Singh RanaOzlem Buyuktanir YasDaniel E. SonenshineUtpal PalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juraj Koči Sandhya Bista Payal Chirania Xiuli Yang Chrysoula Kitsou Vipin Singh Rana Ozlem Buyuktanir Yas Daniel E. Sonenshine Utpal Pal Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
description	Abstract Ixodes scapularis ticks transmit multiple pathogens, including Borrelia burgdorferi sensu stricto, and encode many proteins harboring epidermal growth factor (EGF)-like domains. We show that I. scapularis produces multiple orthologs for Bm86, a widely studied tick gut protein considered as a target of an anti-tick vaccine, herein termed as Is86. We show that Is86 antigens feature at least three identifiable regions harboring EGF-like domains (termed as EGF-1, EGF-2, and EGF-3) and are differentially upregulated during B. burgdorferi infection. Although the RNA interference-mediated knockdown of Is86 genes did not show any influences on tick engorgement or B. burgdorferi sensu stricto persistence, the immunization of murine hosts with specific recombinant EGF antigens marginally reduced spirochete loads in the skin, in addition to affecting tick blood meal engorgement and molting. However, given the borderline impact of EGF immunization on tick engorgement and pathogen survival in the vector, it is unlikely that these antigens, at least in their current forms, could be developed as potential vaccines. Further investigations of the biological significance of Is86 (and other tick antigens) would enrich our knowledge of the intricate biology of ticks, including their interactions with resident pathogens, and contribute to the development of anti-tick measures to combat tick-borne illnesses.
format	article
author	Juraj Koči Sandhya Bista Payal Chirania Xiuli Yang Chrysoula Kitsou Vipin Singh Rana Ozlem Buyuktanir Yas Daniel E. Sonenshine Utpal Pal
author_facet	Juraj Koči Sandhya Bista Payal Chirania Xiuli Yang Chrysoula Kitsou Vipin Singh Rana Ozlem Buyuktanir Yas Daniel E. Sonenshine Utpal Pal
author_sort	Juraj Koči
title	Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
title_short	Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
title_full	Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
title_fullStr	Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
title_full_unstemmed	Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi
title_sort	antibodies against egf-like domains in ixodes scapularis bm86 orthologs impact tick feeding and survival of borrelia burgdorferi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d3ec3b13e732426eba0e7d71824fba0b
work_keys_str_mv	AT jurajkoci antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT sandhyabista antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT payalchirania antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT xiuliyang antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT chrysoulakitsou antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT vipinsinghrana antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT ozlembuyuktaniryas antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT danielesonenshine antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi AT utpalpal antibodiesagainstegflikedomainsinixodesscapularisbm86orthologsimpacttickfeedingandsurvivalofborreliaburgdorferi
_version_	1718395730995970048

Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi

Ejemplares similares