Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells

Abstract Translational diffusion of macromolecules in cell is generally assumed to be anomalous due high macromolecular crowding of the milieu. Red blood cells are a special case of cells filled quasi exclusively (95% of the dry weight of the cell) with an almost spherical protein: hemoglobin. Hemog...

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Autores principales:	Stéphane Longeville, Laura-Roxana Stingaciu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3ed283aee3a4e77b283f115f59fda7b
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spelling	oai:doaj.org-article:d3ed283aee3a4e77b283f115f59fda7b2021-12-02T15:05:25ZHemoglobin diffusion and the dynamics of oxygen capture by red blood cells10.1038/s41598-017-09146-92045-2322https://doaj.org/article/d3ed283aee3a4e77b283f115f59fda7b2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09146-9https://doaj.org/toc/2045-2322Abstract Translational diffusion of macromolecules in cell is generally assumed to be anomalous due high macromolecular crowding of the milieu. Red blood cells are a special case of cells filled quasi exclusively (95% of the dry weight of the cell) with an almost spherical protein: hemoglobin. Hemoglobin diffusion has since a long time been recognized as facilitating the rate of oxygen diffusion through a solution. We address in this paper the question on how hemoglobin diffusion in the red blood cells can help the oxygen capture at the cell level and hence to improve oxygen transport. We report a measurement by neutron spin echo spectroscopy of the diffusion of hemoglobin in solutions with increasing protein concentration. We show that hemoglobin diffusion in solution can be described as Brownian motion up to physiological concentration and that hemoglobin diffusion in the red blood cells and in solutions at similar concentration are the same. Finally, using a simple model and the concentration dependence of the diffusion of the protein reported here, we show that hemoglobin concentration observed in human red blood cells ( $$\simeq $$ ≃ 330 g.L −1) corresponds to an optimum for oxygen transport for individuals under strong activity.Stéphane LongevilleLaura-Roxana StingaciuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stéphane Longeville Laura-Roxana Stingaciu Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
description	Abstract Translational diffusion of macromolecules in cell is generally assumed to be anomalous due high macromolecular crowding of the milieu. Red blood cells are a special case of cells filled quasi exclusively (95% of the dry weight of the cell) with an almost spherical protein: hemoglobin. Hemoglobin diffusion has since a long time been recognized as facilitating the rate of oxygen diffusion through a solution. We address in this paper the question on how hemoglobin diffusion in the red blood cells can help the oxygen capture at the cell level and hence to improve oxygen transport. We report a measurement by neutron spin echo spectroscopy of the diffusion of hemoglobin in solutions with increasing protein concentration. We show that hemoglobin diffusion in solution can be described as Brownian motion up to physiological concentration and that hemoglobin diffusion in the red blood cells and in solutions at similar concentration are the same. Finally, using a simple model and the concentration dependence of the diffusion of the protein reported here, we show that hemoglobin concentration observed in human red blood cells ( $$\simeq $$ ≃ 330 g.L −1) corresponds to an optimum for oxygen transport for individuals under strong activity.
format	article
author	Stéphane Longeville Laura-Roxana Stingaciu
author_facet	Stéphane Longeville Laura-Roxana Stingaciu
author_sort	Stéphane Longeville
title	Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
title_short	Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
title_full	Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
title_fullStr	Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
title_full_unstemmed	Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
title_sort	hemoglobin diffusion and the dynamics of oxygen capture by red blood cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d3ed283aee3a4e77b283f115f59fda7b
work_keys_str_mv	AT stephanelongeville hemoglobindiffusionandthedynamicsofoxygencapturebyredbloodcells AT lauraroxanastingaciu hemoglobindiffusionandthedynamicsofoxygencapturebyredbloodcells
_version_	1718388859393277952

Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells

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