Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells

Aims: Irreversible electroporation is an ablation technique being adapted for the treatment of atrial fibrillation. Currently, there are many differences reported in the in vitro and pre-clinical literature for the effective voltage threshold for ablation. The aim of this study is a direct compariso...

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Autores principales:	Sahar Avazzadeh, Barry O’Brien, Ken Coffey, Martin O’Halloran, David Keane, Leo R. Quinlan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	atrial fibrillation cardiac ablation irreversible electroporation Medicine R
Acceso en línea:	https://doaj.org/article/d3f7531ccefa456fa1f2fc6ed70374a2
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spelling	oai:doaj.org-article:d3f7531ccefa456fa1f2fc6ed70374a22021-11-25T18:02:53ZEstablishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells10.3390/jcm102254432077-0383https://doaj.org/article/d3f7531ccefa456fa1f2fc6ed70374a22021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/22/5443https://doaj.org/toc/2077-0383Aims: Irreversible electroporation is an ablation technique being adapted for the treatment of atrial fibrillation. Currently, there are many differences reported in the in vitro and pre-clinical literature for the effective voltage threshold for ablation. The aim of this study is a direct comparison of different cell types within the cardiovascular system and identification of optimal voltage thresholds for selective cell ablation. Methods: Monophasic voltage pulses were delivered in a cuvette suspension model. Cell viability and live–dead measurements of three different neuronal lines, cardiomyocytes, and cardiac fibroblasts were assessed under different voltage conditions. The immediate effects of voltage and the evolution of cell death was measured at three different time points post ablation. Results: All neuronal and atrial cardiomyocyte lines showed cell viability of less than 20% at an electric field of 1000 V/cm when at least 30 pulses were applied with no significant difference amongst them. In contrast, cardiac fibroblasts showed an optimal threshold at 1250 V/cm with a minimum of 50 pulses. Cell death overtime showed an immediate or delayed cell death with a proportion of cell membranes re-sealing after three hours but no significant difference was observed between treatments after 24 h. Conclusions: The present data suggest that understanding the optimal threshold of irreversible electroporation is vital for achieving a safe ablation modality without any side-effect in nearby cells. Moreover, the evolution of cell death post electroporation is key to obtaining a full understanding of the effects of IRE and selection of an optimal ablation threshold.Sahar AvazzadehBarry O’BrienKen CoffeyMartin O’HalloranDavid KeaneLeo R. QuinlanMDPI AGarticleatrial fibrillationcardiac ablationirreversible electroporationMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5443, p 5443 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	atrial fibrillation cardiac ablation irreversible electroporation Medicine R
spellingShingle	atrial fibrillation cardiac ablation irreversible electroporation Medicine R Sahar Avazzadeh Barry O’Brien Ken Coffey Martin O’Halloran David Keane Leo R. Quinlan Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
description	Aims: Irreversible electroporation is an ablation technique being adapted for the treatment of atrial fibrillation. Currently, there are many differences reported in the in vitro and pre-clinical literature for the effective voltage threshold for ablation. The aim of this study is a direct comparison of different cell types within the cardiovascular system and identification of optimal voltage thresholds for selective cell ablation. Methods: Monophasic voltage pulses were delivered in a cuvette suspension model. Cell viability and live–dead measurements of three different neuronal lines, cardiomyocytes, and cardiac fibroblasts were assessed under different voltage conditions. The immediate effects of voltage and the evolution of cell death was measured at three different time points post ablation. Results: All neuronal and atrial cardiomyocyte lines showed cell viability of less than 20% at an electric field of 1000 V/cm when at least 30 pulses were applied with no significant difference amongst them. In contrast, cardiac fibroblasts showed an optimal threshold at 1250 V/cm with a minimum of 50 pulses. Cell death overtime showed an immediate or delayed cell death with a proportion of cell membranes re-sealing after three hours but no significant difference was observed between treatments after 24 h. Conclusions: The present data suggest that understanding the optimal threshold of irreversible electroporation is vital for achieving a safe ablation modality without any side-effect in nearby cells. Moreover, the evolution of cell death post electroporation is key to obtaining a full understanding of the effects of IRE and selection of an optimal ablation threshold.
format	article
author	Sahar Avazzadeh Barry O’Brien Ken Coffey Martin O’Halloran David Keane Leo R. Quinlan
author_facet	Sahar Avazzadeh Barry O’Brien Ken Coffey Martin O’Halloran David Keane Leo R. Quinlan
author_sort	Sahar Avazzadeh
title	Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
title_short	Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
title_full	Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
title_fullStr	Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
title_full_unstemmed	Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells
title_sort	establishing irreversible electroporation electric field potential threshold in a suspension in vitro model for cardiac and neuronal cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d3f7531ccefa456fa1f2fc6ed70374a2
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Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells

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