Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Abstract Background The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recalcitrance is partly attributed to the complex structure of plant cell wa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang Tian, Chien-Yuan Lin, Joon-Hyun Park, Chuan-Yin Wu, Ramu Kakumanu, Venkataramana R. Pidatala, Khanh M. Vuu, Alberto Rodriguez, Patrick M. Shih, Edward E. K. Baidoo, Stephen Temple, Blake A. Simmons, John M. Gladden, Henrik V. Scheller, Aymerick Eudes
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Sorghum Bioenergy Lignin Xylan Ferulate p-Coumarate Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d3ff27bdda604632aaf7f10e8a8282cd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3ff27bdda604632aaf7f10e8a8282cd
record_format	dspace
spelling	oai:doaj.org-article:d3ff27bdda604632aaf7f10e8a8282cd2021-11-21T12:25:38ZOverexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor10.1186/s13068-021-02068-91754-6834https://doaj.org/article/d3ff27bdda604632aaf7f10e8a8282cd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02068-9https://doaj.org/toc/1754-6834Abstract Background The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recalcitrance is partly attributed to the complex structure of plant cell walls inside which cellulose microfibrils are protected by a network of hemicellulosic xylan chains that crosslink with each other or with lignin via ferulate (FA) bridges. Overexpression of the rice acyltransferase OsAT10 is an effective bioengineering strategy to lower the amount of FA involved in the formation of cell wall crosslinks and thereby reduce cell wall recalcitrance. The annual crop sorghum represents an attractive feedstock for bioenergy purposes considering its high biomass yields and low input requirements. Although we previously validated the OsAT10 engineering approach in the perennial bioenergy crop switchgrass, the effect of OsAT10 expression on biomass composition and digestibility in sorghum remains to be explored. Results We obtained eight independent sorghum (Sorghum bicolor (L.) Moench) transgenic lines with a single copy of a construct designed for OsAT10 expression. Consistent with the proposed role of OsAT10 in acylating arabinosyl residues on xylan with p-coumarate (pCA), a higher amount of p-coumaroyl-arabinose was released from the cell walls of these lines upon hydrolysis with trifluoroacetic acid. However, no major changes were observed regarding the total amount of pCA or FA esters released from cell walls upon mild alkaline hydrolysis. Certain diferulate (diFA) isomers identified in alkaline hydrolysates were increased in some transgenic lines. The amount of the main cell wall monosaccharides glucose, xylose, and arabinose was unaffected. The transgenic lines showed reduced lignin content and their biomass released higher yields of sugars after ionic liquid pretreatment followed by enzymatic saccharification. Conclusions Expression of OsAT10 in sorghum leads to an increase of xylan-bound pCA without reducing the overall content of cell wall FA esters. Nevertheless, the amount of total cell wall pCA remains unchanged indicating that most pCA is ester-linked to lignin. Unlike other engineered plants overexpressing OsAT10 or a phylogenetically related acyltransferase with similar putative function, the improvements of biomass saccharification efficiency in sorghum OsAT10 lines are likely the result of lignin reductions rather than reductions of cell wall-bound FA. These results also suggest a relationship between xylan-bound pCA and lignification in cell walls.Yang TianChien-Yuan LinJoon-Hyun ParkChuan-Yin WuRamu KakumanuVenkataramana R. PidatalaKhanh M. VuuAlberto RodriguezPatrick M. ShihEdward E. K. BaidooStephen TempleBlake A. SimmonsJohn M. GladdenHenrik V. SchellerAymerick EudesBMCarticleSorghumBioenergyLigninXylanFerulatep-CoumarateFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sorghum Bioenergy Lignin Xylan Ferulate p-Coumarate Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Sorghum Bioenergy Lignin Xylan Ferulate p-Coumarate Fuel TP315-360 Biotechnology TP248.13-248.65 Yang Tian Chien-Yuan Lin Joon-Hyun Park Chuan-Yin Wu Ramu Kakumanu Venkataramana R. Pidatala Khanh M. Vuu Alberto Rodriguez Patrick M. Shih Edward E. K. Baidoo Stephen Temple Blake A. Simmons John M. Gladden Henrik V. Scheller Aymerick Eudes Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
description	Abstract Background The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recalcitrance is partly attributed to the complex structure of plant cell walls inside which cellulose microfibrils are protected by a network of hemicellulosic xylan chains that crosslink with each other or with lignin via ferulate (FA) bridges. Overexpression of the rice acyltransferase OsAT10 is an effective bioengineering strategy to lower the amount of FA involved in the formation of cell wall crosslinks and thereby reduce cell wall recalcitrance. The annual crop sorghum represents an attractive feedstock for bioenergy purposes considering its high biomass yields and low input requirements. Although we previously validated the OsAT10 engineering approach in the perennial bioenergy crop switchgrass, the effect of OsAT10 expression on biomass composition and digestibility in sorghum remains to be explored. Results We obtained eight independent sorghum (Sorghum bicolor (L.) Moench) transgenic lines with a single copy of a construct designed for OsAT10 expression. Consistent with the proposed role of OsAT10 in acylating arabinosyl residues on xylan with p-coumarate (pCA), a higher amount of p-coumaroyl-arabinose was released from the cell walls of these lines upon hydrolysis with trifluoroacetic acid. However, no major changes were observed regarding the total amount of pCA or FA esters released from cell walls upon mild alkaline hydrolysis. Certain diferulate (diFA) isomers identified in alkaline hydrolysates were increased in some transgenic lines. The amount of the main cell wall monosaccharides glucose, xylose, and arabinose was unaffected. The transgenic lines showed reduced lignin content and their biomass released higher yields of sugars after ionic liquid pretreatment followed by enzymatic saccharification. Conclusions Expression of OsAT10 in sorghum leads to an increase of xylan-bound pCA without reducing the overall content of cell wall FA esters. Nevertheless, the amount of total cell wall pCA remains unchanged indicating that most pCA is ester-linked to lignin. Unlike other engineered plants overexpressing OsAT10 or a phylogenetically related acyltransferase with similar putative function, the improvements of biomass saccharification efficiency in sorghum OsAT10 lines are likely the result of lignin reductions rather than reductions of cell wall-bound FA. These results also suggest a relationship between xylan-bound pCA and lignification in cell walls.
format	article
author	Yang Tian Chien-Yuan Lin Joon-Hyun Park Chuan-Yin Wu Ramu Kakumanu Venkataramana R. Pidatala Khanh M. Vuu Alberto Rodriguez Patrick M. Shih Edward E. K. Baidoo Stephen Temple Blake A. Simmons John M. Gladden Henrik V. Scheller Aymerick Eudes
author_facet	Yang Tian Chien-Yuan Lin Joon-Hyun Park Chuan-Yin Wu Ramu Kakumanu Venkataramana R. Pidatala Khanh M. Vuu Alberto Rodriguez Patrick M. Shih Edward E. K. Baidoo Stephen Temple Blake A. Simmons John M. Gladden Henrik V. Scheller Aymerick Eudes
author_sort	Yang Tian
title	Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
title_short	Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
title_full	Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
title_fullStr	Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
title_full_unstemmed	Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
title_sort	overexpression of the rice bahd acyltransferase at10 increases xylan-bound p-coumarate and reduces lignin in sorghum bicolor
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d3ff27bdda604632aaf7f10e8a8282cd
work_keys_str_mv	AT yangtian overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT chienyuanlin overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT joonhyunpark overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT chuanyinwu overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT ramukakumanu overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT venkataramanarpidatala overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT khanhmvuu overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT albertorodriguez overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT patrickmshih overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT edwardekbaidoo overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT stephentemple overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT blakeasimmons overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT johnmgladden overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT henrikvscheller overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor AT aymerickeudes overexpressionofthericebahdacyltransferaseat10increasesxylanboundpcoumarateandreduceslignininsorghumbicolor
_version_	1718419033326354432

Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Ejemplares similares