An equatorial contractile mechanism drives cell elongation but not cell division.

Cell shape changes and proliferation are two fundamental strategies for morphogenesis in animal development. During embryogenesis of the simple chordate Ciona intestinalis, elongation of individual notochord cells constitutes a crucial stage of notochord growth, which contributes to the establishmen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ivonne M Sehring, Bo Dong, Elsa Denker, Punit Bhattachan, Wei Deng, Birthe T Mathiesen, Di Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d403246992aa4abcac7228d3d1dba827
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d403246992aa4abcac7228d3d1dba827
record_format	dspace
spelling	oai:doaj.org-article:d403246992aa4abcac7228d3d1dba8272021-11-18T05:37:36ZAn equatorial contractile mechanism drives cell elongation but not cell division.1544-91731545-788510.1371/journal.pbio.1001781https://doaj.org/article/d403246992aa4abcac7228d3d1dba8272014-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24503569/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Cell shape changes and proliferation are two fundamental strategies for morphogenesis in animal development. During embryogenesis of the simple chordate Ciona intestinalis, elongation of individual notochord cells constitutes a crucial stage of notochord growth, which contributes to the establishment of the larval body plan. The mechanism of cell elongation is elusive. Here we show that although notochord cells do not divide, they use a cytokinesis-like actomyosin mechanism to drive cell elongation. The actomyosin network forming at the equator of each notochord cell includes phosphorylated myosin regulatory light chain, α-actinin, cofilin, tropomyosin, and talin. We demonstrate that cofilin and α-actinin are two crucial components for cell elongation. Cortical flow contributes to the assembly of the actomyosin ring. Similar to cytokinetic cells, membrane blebs that cause local contractions form at the basal cortex next to the equator and participate in force generation. We present a model in which the cooperation of equatorial actomyosin ring-based constriction and bleb-associated contractions at the basal cortex promotes cell elongation. Our results demonstrate that a cytokinesis-like contractile mechanism is co-opted in a completely different developmental scenario to achieve cell shape change instead of cell division. We discuss the occurrences of actomyosin rings aside from cell division, suggesting that circumferential contraction is an evolutionally conserved mechanism to drive cell or tissue elongation.Ivonne M SehringBo DongElsa DenkerPunit BhattachanWei DengBirthe T MathiesenDi JiangPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 2, p e1001781 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ivonne M Sehring Bo Dong Elsa Denker Punit Bhattachan Wei Deng Birthe T Mathiesen Di Jiang An equatorial contractile mechanism drives cell elongation but not cell division.
description	Cell shape changes and proliferation are two fundamental strategies for morphogenesis in animal development. During embryogenesis of the simple chordate Ciona intestinalis, elongation of individual notochord cells constitutes a crucial stage of notochord growth, which contributes to the establishment of the larval body plan. The mechanism of cell elongation is elusive. Here we show that although notochord cells do not divide, they use a cytokinesis-like actomyosin mechanism to drive cell elongation. The actomyosin network forming at the equator of each notochord cell includes phosphorylated myosin regulatory light chain, α-actinin, cofilin, tropomyosin, and talin. We demonstrate that cofilin and α-actinin are two crucial components for cell elongation. Cortical flow contributes to the assembly of the actomyosin ring. Similar to cytokinetic cells, membrane blebs that cause local contractions form at the basal cortex next to the equator and participate in force generation. We present a model in which the cooperation of equatorial actomyosin ring-based constriction and bleb-associated contractions at the basal cortex promotes cell elongation. Our results demonstrate that a cytokinesis-like contractile mechanism is co-opted in a completely different developmental scenario to achieve cell shape change instead of cell division. We discuss the occurrences of actomyosin rings aside from cell division, suggesting that circumferential contraction is an evolutionally conserved mechanism to drive cell or tissue elongation.
format	article
author	Ivonne M Sehring Bo Dong Elsa Denker Punit Bhattachan Wei Deng Birthe T Mathiesen Di Jiang
author_facet	Ivonne M Sehring Bo Dong Elsa Denker Punit Bhattachan Wei Deng Birthe T Mathiesen Di Jiang
author_sort	Ivonne M Sehring
title	An equatorial contractile mechanism drives cell elongation but not cell division.
title_short	An equatorial contractile mechanism drives cell elongation but not cell division.
title_full	An equatorial contractile mechanism drives cell elongation but not cell division.
title_fullStr	An equatorial contractile mechanism drives cell elongation but not cell division.
title_full_unstemmed	An equatorial contractile mechanism drives cell elongation but not cell division.
title_sort	equatorial contractile mechanism drives cell elongation but not cell division.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d403246992aa4abcac7228d3d1dba827
work_keys_str_mv	AT ivonnemsehring anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT bodong anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT elsadenker anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT punitbhattachan anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT weideng anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT birthetmathiesen anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT dijiang anequatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT ivonnemsehring equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT bodong equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT elsadenker equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT punitbhattachan equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT weideng equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT birthetmathiesen equatorialcontractilemechanismdrivescellelongationbutnotcelldivision AT dijiang equatorialcontractilemechanismdrivescellelongationbutnotcelldivision
_version_	1718424881029185536

An equatorial contractile mechanism drives cell elongation but not cell division.

Ejemplares similares