Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined...

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Autores principales:	Spencer R Katz, Maksim A Yakovlev, Daniel J Vanselow, Yifu Ding, Alex Y Lin, Dilworth Y Parkinson, Yuxin Wang, Victor A Canfield, Khai C Ang, Keith C Cheng
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	melanin pigmentation micro-CT silver staining 3D imaging whole-organism phenomics Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d40d64fe600d4ee18f7b8f9e921ab330
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spelling	oai:doaj.org-article:d40d64fe600d4ee18f7b8f9e921ab3302021-11-26T07:07:27ZWhole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT10.7554/eLife.689202050-084Xe68920https://doaj.org/article/d40d64fe600d4ee18f7b8f9e921ab3302021-09-01T00:00:00Zhttps://elifesciences.org/articles/68920https://doaj.org/toc/2050-084XWe previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.Spencer R KatzMaksim A YakovlevDaniel J VanselowYifu DingAlex Y LinDilworth Y ParkinsonYuxin WangVictor A CanfieldKhai C AngKeith C ChengeLife Sciences Publications Ltdarticlemelaninpigmentationmicro-CTsilver staining3D imagingwhole-organism phenomicsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanin pigmentation micro-CT silver staining 3D imaging whole-organism phenomics Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	melanin pigmentation micro-CT silver staining 3D imaging whole-organism phenomics Medicine R Science Q Biology (General) QH301-705.5 Spencer R Katz Maksim A Yakovlev Daniel J Vanselow Yifu Ding Alex Y Lin Dilworth Y Parkinson Yuxin Wang Victor A Canfield Khai C Ang Keith C Cheng Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
description	We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.
format	article
author	Spencer R Katz Maksim A Yakovlev Daniel J Vanselow Yifu Ding Alex Y Lin Dilworth Y Parkinson Yuxin Wang Victor A Canfield Khai C Ang Keith C Cheng
author_facet	Spencer R Katz Maksim A Yakovlev Daniel J Vanselow Yifu Ding Alex Y Lin Dilworth Y Parkinson Yuxin Wang Victor A Canfield Khai C Ang Keith C Cheng
author_sort	Spencer R Katz
title	Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
title_short	Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
title_full	Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
title_fullStr	Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
title_full_unstemmed	Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
title_sort	whole-organism 3d quantitative characterization of zebrafish melanin by silver deposition micro-ct
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/d40d64fe600d4ee18f7b8f9e921ab330
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_version_	1718409746636079104

Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

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