Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis

Abstract The biotechnological production of the carotenoid astaxanthin is done with the microalgae Haematococcus pluvialis (H. pluvialis). Under nutrient deficiency and light stress, H. pluvialis accumulates astaxanthin intracellularly and forms a resistant cyst cell wall that impedes direct astaxan...

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Autores principales:	Andreas Bauer, Mirjana Minceva
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Haematococcus pluvialis Astaxanthin Techno-economic analysis Liquid–liquid chromatography Centrifugal partition extraction Downstream processing Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d40e67deee324c7f81ad80b20259fbb5
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spelling	oai:doaj.org-article:d40e67deee324c7f81ad80b20259fbb52021-11-14T12:06:01ZTechno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis10.1186/s40643-021-00463-62197-4365https://doaj.org/article/d40e67deee324c7f81ad80b20259fbb52021-11-01T00:00:00Zhttps://doi.org/10.1186/s40643-021-00463-6https://doaj.org/toc/2197-4365Abstract The biotechnological production of the carotenoid astaxanthin is done with the microalgae Haematococcus pluvialis (H. pluvialis). Under nutrient deficiency and light stress, H. pluvialis accumulates astaxanthin intracellularly and forms a resistant cyst cell wall that impedes direct astaxanthin extraction. Therefore, a complex downstream process is required, including centrifugation, mechanical cell wall disruption, drying, and supercritical extraction of astaxanthin with CO2. In this work, an alternative downstream process based on the direct extraction of astaxanthin from the algal broth into ethyl acetate using a centrifugal partition extractor (CPE) was developed. A mechanical cell wall disruption or germination of the cysts was carried out to make astaxanthin accessible to the solvent. Zoospores containing astaxanthin are released when growth conditions are applied to cyst cells, from which astaxanthin can directly be extracted into ethyl acetate. Energy-intensive unit operations such as spray-drying and extraction with supercritical CO2 can be replaced by directly extracting astaxanthin into ethyl acetate. Extraction yields of 85% were reached, and 3.5 g of oleoresin could be extracted from 7.85 g homogenised H. pluvialis biomass using a CPE unit with 244 mL column volume. A techno-economic analysis was done for a hypothetical H. pluvialis production facility with an annual biomass output of 8910 kg. Four downstream scenarios were examined, comparing the novel process of astaxanthin extraction from homogenised cyst cells and germinated zoospores via CPE extraction with the conventional industrial process using in-house or supercritical CO2 extraction via an external service provider. After 10 years of operation, the highest net present value (NPV) was determined for the CPE extraction from germinated zoospores.Andreas BauerMirjana MincevaSpringerOpenarticleHaematococcus pluvialisAstaxanthinTechno-economic analysisLiquid–liquid chromatographyCentrifugal partition extractionDownstream processingTechnologyTChemical technologyTP1-1185BiotechnologyTP248.13-248.65ENBioresources and Bioprocessing, Vol 8, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Haematococcus pluvialis Astaxanthin Techno-economic analysis Liquid–liquid chromatography Centrifugal partition extraction Downstream processing Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
spellingShingle	Haematococcus pluvialis Astaxanthin Techno-economic analysis Liquid–liquid chromatography Centrifugal partition extraction Downstream processing Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Andreas Bauer Mirjana Minceva Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
description	Abstract The biotechnological production of the carotenoid astaxanthin is done with the microalgae Haematococcus pluvialis (H. pluvialis). Under nutrient deficiency and light stress, H. pluvialis accumulates astaxanthin intracellularly and forms a resistant cyst cell wall that impedes direct astaxanthin extraction. Therefore, a complex downstream process is required, including centrifugation, mechanical cell wall disruption, drying, and supercritical extraction of astaxanthin with CO2. In this work, an alternative downstream process based on the direct extraction of astaxanthin from the algal broth into ethyl acetate using a centrifugal partition extractor (CPE) was developed. A mechanical cell wall disruption or germination of the cysts was carried out to make astaxanthin accessible to the solvent. Zoospores containing astaxanthin are released when growth conditions are applied to cyst cells, from which astaxanthin can directly be extracted into ethyl acetate. Energy-intensive unit operations such as spray-drying and extraction with supercritical CO2 can be replaced by directly extracting astaxanthin into ethyl acetate. Extraction yields of 85% were reached, and 3.5 g of oleoresin could be extracted from 7.85 g homogenised H. pluvialis biomass using a CPE unit with 244 mL column volume. A techno-economic analysis was done for a hypothetical H. pluvialis production facility with an annual biomass output of 8910 kg. Four downstream scenarios were examined, comparing the novel process of astaxanthin extraction from homogenised cyst cells and germinated zoospores via CPE extraction with the conventional industrial process using in-house or supercritical CO2 extraction via an external service provider. After 10 years of operation, the highest net present value (NPV) was determined for the CPE extraction from germinated zoospores.
format	article
author	Andreas Bauer Mirjana Minceva
author_facet	Andreas Bauer Mirjana Minceva
author_sort	Andreas Bauer
title	Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
title_short	Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
title_full	Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
title_fullStr	Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
title_full_unstemmed	Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis
title_sort	techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae haematococcus pluvialis
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/d40e67deee324c7f81ad80b20259fbb5
work_keys_str_mv	AT andreasbauer technoeconomicanalysisofanewdownstreamprocessfortheproductionofastaxanthinfromthemicroalgaehaematococcuspluvialis AT mirjanaminceva technoeconomicanalysisofanewdownstreamprocessfortheproductionofastaxanthinfromthemicroalgaehaematococcuspluvialis
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Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis

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