The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>

Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a ba...

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Autores principales:	Xiaowei Gao, Bhagyashree N. Swarge, Henk L. Dekker, Winfried Roseboom, Stanley Brul, Gertjan Kramer
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bacillus cereus</i> spore inner membrane proteome Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d40e708adac44cbd888ea30063ce4273
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spelling	oai:doaj.org-article:d40e708adac44cbd888ea30063ce42732021-11-25T17:56:59ZThe Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>10.3390/ijms2222124751422-00671661-6596https://doaj.org/article/d40e708adac44cbd888ea30063ce42732021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12475https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen <i>Bacillus cereus</i> using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane-specific and 308 cell membrane-specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptors and spore-specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore-forming bacterium to survive adverse conditions.Xiaowei GaoBhagyashree N. SwargeHenk L. DekkerWinfried RoseboomStanley BrulGertjan KramerMDPI AGarticle<i>Bacillus cereus</i>spore inner membraneproteomeBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12475, p 12475 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bacillus cereus</i> spore inner membrane proteome Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Bacillus cereus</i> spore inner membrane proteome Biology (General) QH301-705.5 Chemistry QD1-999 Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
description	Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen <i>Bacillus cereus</i> using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane-specific and 308 cell membrane-specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptors and spore-specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore-forming bacterium to survive adverse conditions.
format	article
author	Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer
author_facet	Xiaowei Gao Bhagyashree N. Swarge Henk L. Dekker Winfried Roseboom Stanley Brul Gertjan Kramer
author_sort	Xiaowei Gao
title	The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
title_short	The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
title_full	The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
title_fullStr	The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
title_full_unstemmed	The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>
title_sort	membrane proteome of spores and vegetative cells of the food-borne pathogen <i>bacillus cereus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d40e708adac44cbd888ea30063ce4273
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The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen <i>Bacillus cereus</i>

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