Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19

Abstract Background The purpose of this study was to explore whether moderate-intensity exercise can alleviate motion-induced post-traumatic osteoarthritis (PTOA) and the expression change of lncRNA H19 during this progression. Methods Twenty-week-old male C57BL/6 mice were randomly divided into fiv...

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Autores principales:	Xuchang Zhou, Hong Cao, Miao Wang, Jun Zou, Wei Wu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Post-traumatic osteoarthritis lncRNA H19 Subchondral bone Cartilage Treadmill running Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/d40ee2534316457d9e562242c30eb29a
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spelling	oai:doaj.org-article:d40ee2534316457d9e562242c30eb29a2021-11-21T12:12:18ZModerate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H1910.1186/s12938-021-00949-61475-925Xhttps://doaj.org/article/d40ee2534316457d9e562242c30eb29a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12938-021-00949-6https://doaj.org/toc/1475-925XAbstract Background The purpose of this study was to explore whether moderate-intensity exercise can alleviate motion-induced post-traumatic osteoarthritis (PTOA) and the expression change of lncRNA H19 during this progression. Methods Twenty-week-old male C57BL/6 mice were randomly divided into five groups: model control group (MC group, n = 6), treadmill model group (M group, n = 6), rehabilitation control group (RC group, n = 6), treadmill model + rehabilitation training group (M + R group, n = 6) and treadmill model + convalescent group (M + C group, n = 6). Paraffin sections were used to observe the pathological changes in the mouse knee joint in each group. A micro-CT was used to scan the knee joint to obtain the morphological indexes of the tibial plateau bone. Real-time PCR was used to detect the mRNA levels of inflammatory factors, synthetic and catabolic factors in cartilage. Results After high-intensity exercise for 4 weeks, the inflammation and catabolism of the mouse knee cartilage were enhanced, and the anabolism was weakened. Further study showed that these results were partially reversed after 4-week moderate-intensity training. The results of hematoxylin–eosin staining confirmed this finding. Meanwhile, high-intensity exercise reduced the expression of lncRNA H19 in cartilage, while the expression of lncRNA H19 increased after 4 weeks of moderate-intensity exercise. Conclusion High-intensity treadmill running can cause injury to the knee cartilage in C57BL/6 mice which leads to PTOA and a decrease of lncRNA H19 expression in cartilage. Moderate-intensity exercise can relieve PTOA and partially reverse lncRNA H19 expression.Xuchang ZhouHong CaoMiao WangJun ZouWei WuBMCarticlePost-traumatic osteoarthritislncRNA H19Subchondral boneCartilageTreadmill runningMedical technologyR855-855.5ENBioMedical Engineering OnLine, Vol 20, Iss 1, Pp 1-22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Post-traumatic osteoarthritis lncRNA H19 Subchondral bone Cartilage Treadmill running Medical technology R855-855.5
spellingShingle	Post-traumatic osteoarthritis lncRNA H19 Subchondral bone Cartilage Treadmill running Medical technology R855-855.5 Xuchang Zhou Hong Cao Miao Wang Jun Zou Wei Wu Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
description	Abstract Background The purpose of this study was to explore whether moderate-intensity exercise can alleviate motion-induced post-traumatic osteoarthritis (PTOA) and the expression change of lncRNA H19 during this progression. Methods Twenty-week-old male C57BL/6 mice were randomly divided into five groups: model control group (MC group, n = 6), treadmill model group (M group, n = 6), rehabilitation control group (RC group, n = 6), treadmill model + rehabilitation training group (M + R group, n = 6) and treadmill model + convalescent group (M + C group, n = 6). Paraffin sections were used to observe the pathological changes in the mouse knee joint in each group. A micro-CT was used to scan the knee joint to obtain the morphological indexes of the tibial plateau bone. Real-time PCR was used to detect the mRNA levels of inflammatory factors, synthetic and catabolic factors in cartilage. Results After high-intensity exercise for 4 weeks, the inflammation and catabolism of the mouse knee cartilage were enhanced, and the anabolism was weakened. Further study showed that these results were partially reversed after 4-week moderate-intensity training. The results of hematoxylin–eosin staining confirmed this finding. Meanwhile, high-intensity exercise reduced the expression of lncRNA H19 in cartilage, while the expression of lncRNA H19 increased after 4 weeks of moderate-intensity exercise. Conclusion High-intensity treadmill running can cause injury to the knee cartilage in C57BL/6 mice which leads to PTOA and a decrease of lncRNA H19 expression in cartilage. Moderate-intensity exercise can relieve PTOA and partially reverse lncRNA H19 expression.
format	article
author	Xuchang Zhou Hong Cao Miao Wang Jun Zou Wei Wu
author_facet	Xuchang Zhou Hong Cao Miao Wang Jun Zou Wei Wu
author_sort	Xuchang Zhou
title	Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
title_short	Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
title_full	Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
title_fullStr	Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
title_full_unstemmed	Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19
title_sort	moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncrna h19
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d40ee2534316457d9e562242c30eb29a
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Moderate-intensity treadmill running relieves motion-induced post-traumatic osteoarthritis mice by up-regulating the expression of lncRNA H19

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