Nanodelivery of a functional membrane receptor to manipulate cellular phenotype

Abstract Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here...

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Autores principales:	Tommaso Patriarchi, Ao Shen, Wei He, Mo Baikoghli, R. Holland Cheng, Yang K. Xiang, Matthew A. Coleman, Lin Tian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4115b454b4245c79e61e876075b3332
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spelling	oai:doaj.org-article:d4115b454b4245c79e61e876075b33322021-12-02T11:40:54ZNanodelivery of a functional membrane receptor to manipulate cellular phenotype10.1038/s41598-018-21863-32045-2322https://doaj.org/article/d4115b454b4245c79e61e876075b33322018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21863-3https://doaj.org/toc/2045-2322Abstract Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β2-adrenergic receptor (β2AR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length β2AR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine.Tommaso PatriarchiAo ShenWei HeMo BaikoghliR. Holland ChengYang K. XiangMatthew A. ColemanLin TianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tommaso Patriarchi Ao Shen Wei He Mo Baikoghli R. Holland Cheng Yang K. Xiang Matthew A. Coleman Lin Tian Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
description	Abstract Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β2-adrenergic receptor (β2AR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length β2AR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine.
format	article
author	Tommaso Patriarchi Ao Shen Wei He Mo Baikoghli R. Holland Cheng Yang K. Xiang Matthew A. Coleman Lin Tian
author_facet	Tommaso Patriarchi Ao Shen Wei He Mo Baikoghli R. Holland Cheng Yang K. Xiang Matthew A. Coleman Lin Tian
author_sort	Tommaso Patriarchi
title	Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
title_short	Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
title_full	Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
title_fullStr	Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
title_full_unstemmed	Nanodelivery of a functional membrane receptor to manipulate cellular phenotype
title_sort	nanodelivery of a functional membrane receptor to manipulate cellular phenotype
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d4115b454b4245c79e61e876075b3332
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Nanodelivery of a functional membrane receptor to manipulate cellular phenotype

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