Conformational spread in the flagellar motor switch: a model study.

The reliable response to weak biological signals requires that they be amplified with fidelity. In E. coli, the flagellar motors that control swimming can switch direction in response to very small changes in the concentration of the signaling protein CheY-P, but how this works is not well understoo...

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Autores principales:	Qi Ma, Dan V Nicolau, Philip K Maini, Richard M Berry, Fan Bai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d412d22be5b348c0a9371ff7c6aed3c6
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spelling	oai:doaj.org-article:d412d22be5b348c0a9371ff7c6aed3c62021-11-18T05:51:19ZConformational spread in the flagellar motor switch: a model study.1553-734X1553-735810.1371/journal.pcbi.1002523https://doaj.org/article/d412d22be5b348c0a9371ff7c6aed3c62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22654654/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The reliable response to weak biological signals requires that they be amplified with fidelity. In E. coli, the flagellar motors that control swimming can switch direction in response to very small changes in the concentration of the signaling protein CheY-P, but how this works is not well understood. A recently proposed allosteric model based on cooperative conformational spread in a ring of identical protomers seems promising as it is able to qualitatively reproduce switching, locked state behavior and Hill coefficient values measured for the rotary motor. In this paper we undertook a comprehensive simulation study to analyze the behavior of this model in detail and made predictions on three experimentally observable quantities: switch time distribution, locked state interval distribution, Hill coefficient of the switch response. We parameterized the model using experimental measurements, finding excellent agreement with published data on motor behavior. Analysis of the simulated switching dynamics revealed a mechanism for chemotactic ultrasensitivity, in which cooperativity is indispensable for realizing both coherent switching and effective amplification. These results showed how cells can combine elements of analog and digital control to produce switches that are simultaneously sensitive and reliable.Qi MaDan V NicolauPhilip K MainiRichard M BerryFan BaiPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 8, Iss 5, p e1002523 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Qi Ma Dan V Nicolau Philip K Maini Richard M Berry Fan Bai Conformational spread in the flagellar motor switch: a model study.
description	The reliable response to weak biological signals requires that they be amplified with fidelity. In E. coli, the flagellar motors that control swimming can switch direction in response to very small changes in the concentration of the signaling protein CheY-P, but how this works is not well understood. A recently proposed allosteric model based on cooperative conformational spread in a ring of identical protomers seems promising as it is able to qualitatively reproduce switching, locked state behavior and Hill coefficient values measured for the rotary motor. In this paper we undertook a comprehensive simulation study to analyze the behavior of this model in detail and made predictions on three experimentally observable quantities: switch time distribution, locked state interval distribution, Hill coefficient of the switch response. We parameterized the model using experimental measurements, finding excellent agreement with published data on motor behavior. Analysis of the simulated switching dynamics revealed a mechanism for chemotactic ultrasensitivity, in which cooperativity is indispensable for realizing both coherent switching and effective amplification. These results showed how cells can combine elements of analog and digital control to produce switches that are simultaneously sensitive and reliable.
format	article
author	Qi Ma Dan V Nicolau Philip K Maini Richard M Berry Fan Bai
author_facet	Qi Ma Dan V Nicolau Philip K Maini Richard M Berry Fan Bai
author_sort	Qi Ma
title	Conformational spread in the flagellar motor switch: a model study.
title_short	Conformational spread in the flagellar motor switch: a model study.
title_full	Conformational spread in the flagellar motor switch: a model study.
title_fullStr	Conformational spread in the flagellar motor switch: a model study.
title_full_unstemmed	Conformational spread in the flagellar motor switch: a model study.
title_sort	conformational spread in the flagellar motor switch: a model study.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d412d22be5b348c0a9371ff7c6aed3c6
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Conformational spread in the flagellar motor switch: a model study.

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