Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with go...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/d4135205d05a4b3b94a64a6af05339e8 |
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| Sumario: | In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with gold nanoparticles (AuNPs) were set as the fluorescence switch off reagent, while acetamiprid acts as the fluorescence turn-on reagent. After optimization, the concentration of acetamiprid producing 50% saturation of the signal (SC50) was 0.04 µg/L, and the detection range (SC10–SC90) was 0.002–0.58 µg/L. The IFE-IA showed no cross-reactivity with the analogues of acetamiprid except for thiacloprid (36.4%). The average recoveries of the immunoassay for spiked samples were in the range of 75.1–104.7%. In addition, the assay showed good correlation with high-performance liquid chromatography in the detection of blind samples. |
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