Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles

In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with go...

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Autores principales:	Jiao Li, Wanlin Sun, Yuling Qin, Panpan Cui, Guangyue Song, Xiude Hua, Limin Wang, Minghua Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	acetamiprid immunoassay inner filter effect gold nanoparticles upconversion nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d4135205d05a4b3b94a64a6af05339e8
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spelling	oai:doaj.org-article:d4135205d05a4b3b94a64a6af05339e82021-11-04T15:00:41ZInner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles0954-01051465-344310.1080/09540105.2021.1991281https://doaj.org/article/d4135205d05a4b3b94a64a6af05339e82021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2021.1991281https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with gold nanoparticles (AuNPs) were set as the fluorescence switch off reagent, while acetamiprid acts as the fluorescence turn-on reagent. After optimization, the concentration of acetamiprid producing 50% saturation of the signal (SC50) was 0.04 µg/L, and the detection range (SC10–SC90) was 0.002–0.58 µg/L. The IFE-IA showed no cross-reactivity with the analogues of acetamiprid except for thiacloprid (36.4%). The average recoveries of the immunoassay for spiked samples were in the range of 75.1–104.7%. In addition, the assay showed good correlation with high-performance liquid chromatography in the detection of blind samples.Jiao LiWanlin SunYuling QinPanpan CuiGuangyue SongXiude HuaLimin WangMinghua WangTaylor & Francis Grouparticleacetamipridimmunoassayinner filter effectgold nanoparticlesupconversion nanoparticlesAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 32, Iss 1, Pp 740-753 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acetamiprid immunoassay inner filter effect gold nanoparticles upconversion nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	acetamiprid immunoassay inner filter effect gold nanoparticles upconversion nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Jiao Li Wanlin Sun Yuling Qin Panpan Cui Guangyue Song Xiude Hua Limin Wang Minghua Wang Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
description	In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off–on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with gold nanoparticles (AuNPs) were set as the fluorescence switch off reagent, while acetamiprid acts as the fluorescence turn-on reagent. After optimization, the concentration of acetamiprid producing 50% saturation of the signal (SC50) was 0.04 µg/L, and the detection range (SC10–SC90) was 0.002–0.58 µg/L. The IFE-IA showed no cross-reactivity with the analogues of acetamiprid except for thiacloprid (36.4%). The average recoveries of the immunoassay for spiked samples were in the range of 75.1–104.7%. In addition, the assay showed good correlation with high-performance liquid chromatography in the detection of blind samples.
format	article
author	Jiao Li Wanlin Sun Yuling Qin Panpan Cui Guangyue Song Xiude Hua Limin Wang Minghua Wang
author_facet	Jiao Li Wanlin Sun Yuling Qin Panpan Cui Guangyue Song Xiude Hua Limin Wang Minghua Wang
author_sort	Jiao Li
title	Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
title_short	Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
title_full	Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
title_fullStr	Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
title_full_unstemmed	Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
title_sort	inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d4135205d05a4b3b94a64a6af05339e8
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Inner filter effect-based immunoassay for the detection of acetamiprid using upconversion nanoparticles and gold nanoparticles

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