Long-term survival of hydrated resting eggs from Brachionus plicatilis.

<h4>Background</h4>Several organisms display dormancy and developmental arrest at embryonic stages. Long-term survival in the dormant form is usually associated with desiccation, orthodox plant seeds and Artemia cysts being well documented examples. Several aquatic invertebrates display...

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Autores principales:	Melody S Clark, Nadav Y Denekamp, Michael A S Thorne, Richard Reinhardt, Mario Drungowski, Marcus W Albrecht, Sven Klages, Alfred Beck, Michael Kube, Esther Lubzens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d413d9dde8094a118a15f086d136c6f7
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spelling	oai:doaj.org-article:d413d9dde8094a118a15f086d136c6f72021-11-18T07:30:43ZLong-term survival of hydrated resting eggs from Brachionus plicatilis.1932-620310.1371/journal.pone.0029365https://doaj.org/article/d413d9dde8094a118a15f086d136c6f72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253713/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Several organisms display dormancy and developmental arrest at embryonic stages. Long-term survival in the dormant form is usually associated with desiccation, orthodox plant seeds and Artemia cysts being well documented examples. Several aquatic invertebrates display dormancy during embryonic development and survive for tens or even hundreds of years in a hydrated form, raising the question of whether survival in the non-desiccated form of embryonic development depends on pathways similar to those occurring in desiccation tolerant forms.<h4>Methodology/principal findings</h4>To address this question, Illumina short read sequencing was used to generate transcription profiles from the resting and amictic eggs of an aquatic invertebrate, the rotifer, Brachionus plicatilis. These two types of egg have very different life histories, with the dormant or diapausing resting eggs, the result of the sexual cycle and amictic eggs, the non-dormant products of the asexual cycle. Significant transcriptional differences were found between the two types of egg, with amictic eggs rich in genes involved in the morphological development into a juvenile rotifer. In contrast, representatives of classical "stress" proteins: a small heat shock protein, ferritin and Late Embryogenesis Abundant (LEA) proteins were identified in resting eggs. More importantly however, was the identification of transcripts for messenger ribonucleoprotein particles which stabilise RNA. These inhibit translation and provide a valuable source of useful RNAs which can be rapidly activated on the exit from dormancy. Apoptotic genes were also present. Although apoptosis is inconsistent with maintenance of prolonged dormancy, an altered apoptotic pathway has been proposed for Artemia, and this may be the case with the rotifer.<h4>Conclusions</h4>These data represent the first transcriptional profiling of molecular processes associated with dormancy in a non-desiccated form and indicate important similarities in the molecular pathways activated in resting eggs compared with desiccated dormant forms, specifically plant seeds and Artemia.Melody S ClarkNadav Y DenekampMichael A S ThorneRichard ReinhardtMario DrungowskiMarcus W AlbrechtSven KlagesAlfred BeckMichael KubeEsther LubzensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29365 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melody S Clark Nadav Y Denekamp Michael A S Thorne Richard Reinhardt Mario Drungowski Marcus W Albrecht Sven Klages Alfred Beck Michael Kube Esther Lubzens Long-term survival of hydrated resting eggs from Brachionus plicatilis.
description	<h4>Background</h4>Several organisms display dormancy and developmental arrest at embryonic stages. Long-term survival in the dormant form is usually associated with desiccation, orthodox plant seeds and Artemia cysts being well documented examples. Several aquatic invertebrates display dormancy during embryonic development and survive for tens or even hundreds of years in a hydrated form, raising the question of whether survival in the non-desiccated form of embryonic development depends on pathways similar to those occurring in desiccation tolerant forms.<h4>Methodology/principal findings</h4>To address this question, Illumina short read sequencing was used to generate transcription profiles from the resting and amictic eggs of an aquatic invertebrate, the rotifer, Brachionus plicatilis. These two types of egg have very different life histories, with the dormant or diapausing resting eggs, the result of the sexual cycle and amictic eggs, the non-dormant products of the asexual cycle. Significant transcriptional differences were found between the two types of egg, with amictic eggs rich in genes involved in the morphological development into a juvenile rotifer. In contrast, representatives of classical "stress" proteins: a small heat shock protein, ferritin and Late Embryogenesis Abundant (LEA) proteins were identified in resting eggs. More importantly however, was the identification of transcripts for messenger ribonucleoprotein particles which stabilise RNA. These inhibit translation and provide a valuable source of useful RNAs which can be rapidly activated on the exit from dormancy. Apoptotic genes were also present. Although apoptosis is inconsistent with maintenance of prolonged dormancy, an altered apoptotic pathway has been proposed for Artemia, and this may be the case with the rotifer.<h4>Conclusions</h4>These data represent the first transcriptional profiling of molecular processes associated with dormancy in a non-desiccated form and indicate important similarities in the molecular pathways activated in resting eggs compared with desiccated dormant forms, specifically plant seeds and Artemia.
format	article
author	Melody S Clark Nadav Y Denekamp Michael A S Thorne Richard Reinhardt Mario Drungowski Marcus W Albrecht Sven Klages Alfred Beck Michael Kube Esther Lubzens
author_facet	Melody S Clark Nadav Y Denekamp Michael A S Thorne Richard Reinhardt Mario Drungowski Marcus W Albrecht Sven Klages Alfred Beck Michael Kube Esther Lubzens
author_sort	Melody S Clark
title	Long-term survival of hydrated resting eggs from Brachionus plicatilis.
title_short	Long-term survival of hydrated resting eggs from Brachionus plicatilis.
title_full	Long-term survival of hydrated resting eggs from Brachionus plicatilis.
title_fullStr	Long-term survival of hydrated resting eggs from Brachionus plicatilis.
title_full_unstemmed	Long-term survival of hydrated resting eggs from Brachionus plicatilis.
title_sort	long-term survival of hydrated resting eggs from brachionus plicatilis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d413d9dde8094a118a15f086d136c6f7
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Long-term survival of hydrated resting eggs from Brachionus plicatilis.

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