Ubiquitin-like protein from human placental extract exhibits collagenase activity.

An aqueous extract of human placenta exhibits strong gelatinase/collagenase activity in zymography. 2-D gel electrophoresis of the extract with gelatin zymography in the second dimension displayed a single spot, identified as ubiquitin-like component upon MALDI/TOF MS/MS analysis. Immunoblot indicat...

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Autores principales:	Debashree De, Piyali Datta Chakraborty, Jyotirmoy Mitra, Kanika Sharma, Somnath Mandal, Aneesha Das, Saikat Chakrabarti, Debasish Bhattacharyya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d414e310bf3f432e806c9a6a4b719936
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spelling	oai:doaj.org-article:d414e310bf3f432e806c9a6a4b7199362021-11-18T07:51:59ZUbiquitin-like protein from human placental extract exhibits collagenase activity.1932-620310.1371/journal.pone.0059585https://doaj.org/article/d414e310bf3f432e806c9a6a4b7199362013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555718/?tool=EBIhttps://doaj.org/toc/1932-6203An aqueous extract of human placenta exhibits strong gelatinase/collagenase activity in zymography. 2-D gel electrophoresis of the extract with gelatin zymography in the second dimension displayed a single spot, identified as ubiquitin-like component upon MALDI/TOF MS/MS analysis. Immunoblot indicated presence of ubiquitin and absence of collagenase in the extract. Collagenase activity of the ubiquitin-like component was confirmed from the change in solubility of collagen in aqueous buffer, degradation of collagen by size-exclusion HPLC and atomic force microscopy. Quantification with DQ-gelatin showed that the extract contains 0.04 U/ml of collagenase activity that was inhibited up to 95% by ubiquitin antibody. Ubiquitin from bovine erythrocytes demonstrated mild collagenase activity. Bioinformatics studies suggest that placental ubiquitin and collagenase follow structurally divergent evolution. This thermostable intrinsic collagenase activity of placental extract might have wide physiological relevance in degrading and remodeling collagen as it is used as a drug for wound healing and pelvic inflammatory diseases.Debashree DePiyali Datta ChakrabortyJyotirmoy MitraKanika SharmaSomnath MandalAneesha DasSaikat ChakrabartiDebasish BhattacharyyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59585 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Debashree De Piyali Datta Chakraborty Jyotirmoy Mitra Kanika Sharma Somnath Mandal Aneesha Das Saikat Chakrabarti Debasish Bhattacharyya Ubiquitin-like protein from human placental extract exhibits collagenase activity.
description	An aqueous extract of human placenta exhibits strong gelatinase/collagenase activity in zymography. 2-D gel electrophoresis of the extract with gelatin zymography in the second dimension displayed a single spot, identified as ubiquitin-like component upon MALDI/TOF MS/MS analysis. Immunoblot indicated presence of ubiquitin and absence of collagenase in the extract. Collagenase activity of the ubiquitin-like component was confirmed from the change in solubility of collagen in aqueous buffer, degradation of collagen by size-exclusion HPLC and atomic force microscopy. Quantification with DQ-gelatin showed that the extract contains 0.04 U/ml of collagenase activity that was inhibited up to 95% by ubiquitin antibody. Ubiquitin from bovine erythrocytes demonstrated mild collagenase activity. Bioinformatics studies suggest that placental ubiquitin and collagenase follow structurally divergent evolution. This thermostable intrinsic collagenase activity of placental extract might have wide physiological relevance in degrading and remodeling collagen as it is used as a drug for wound healing and pelvic inflammatory diseases.
format	article
author	Debashree De Piyali Datta Chakraborty Jyotirmoy Mitra Kanika Sharma Somnath Mandal Aneesha Das Saikat Chakrabarti Debasish Bhattacharyya
author_facet	Debashree De Piyali Datta Chakraborty Jyotirmoy Mitra Kanika Sharma Somnath Mandal Aneesha Das Saikat Chakrabarti Debasish Bhattacharyya
author_sort	Debashree De
title	Ubiquitin-like protein from human placental extract exhibits collagenase activity.
title_short	Ubiquitin-like protein from human placental extract exhibits collagenase activity.
title_full	Ubiquitin-like protein from human placental extract exhibits collagenase activity.
title_fullStr	Ubiquitin-like protein from human placental extract exhibits collagenase activity.
title_full_unstemmed	Ubiquitin-like protein from human placental extract exhibits collagenase activity.
title_sort	ubiquitin-like protein from human placental extract exhibits collagenase activity.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d414e310bf3f432e806c9a6a4b719936
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Ubiquitin-like protein from human placental extract exhibits collagenase activity.

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