Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same me...

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Autores principales:	Simona M. Miron, Ariane de Espindola, Patrick Dutournié, Arnaud Ponche
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d417bdbb17c847fc80a40bfa19f6045e
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spelling	oai:doaj.org-article:d417bdbb17c847fc80a40bfa19f6045e2021-12-02T17:52:42ZStudy of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme10.1038/s41598-021-91564-x2045-2322https://doaj.org/article/d417bdbb17c847fc80a40bfa19f6045e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91564-xhttps://doaj.org/toc/2045-2322Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.Simona M. MironAriane de EspindolaPatrick DutourniéArnaud PoncheNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simona M. Miron Ariane de Espindola Patrick Dutournié Arnaud Ponche Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
description	Abstract During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.
format	article
author	Simona M. Miron Ariane de Espindola Patrick Dutournié Arnaud Ponche
author_facet	Simona M. Miron Ariane de Espindola Patrick Dutournié Arnaud Ponche
author_sort	Simona M. Miron
title	Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_short	Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_full	Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_fullStr	Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_full_unstemmed	Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
title_sort	study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d417bdbb17c847fc80a40bfa19f6045e
work_keys_str_mv	AT simonammiron studyoftherelationshipbetweenappliedtransmembranepressureandantimicrobialactivityoflysozyme AT arianedeespindola studyoftherelationshipbetweenappliedtransmembranepressureandantimicrobialactivityoflysozyme AT patrickdutournie studyoftherelationshipbetweenappliedtransmembranepressureandantimicrobialactivityoflysozyme AT arnaudponche studyoftherelationshipbetweenappliedtransmembranepressureandantimicrobialactivityoflysozyme
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Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

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