Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells

The SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell en...

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Autores principales:	Flávia Bessi Constantino, Sarah Santiloni Cury, Celia Regina Nogueira, Robson Francisco Carvalho, Luis Antonio Justulin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	COVID-19 SARS-CoV-2 placenta virus entry mediator DPP4 CTSL Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d41b158c3d734d92bae4e18b8d8e1b44
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spelling	oai:doaj.org-article:d41b158c3d734d92bae4e18b8d8e1b442021-11-08T08:10:04ZPrediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells2296-889X10.3389/fmolb.2021.614728https://doaj.org/article/d41b158c3d734d92bae4e18b8d8e1b442021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.614728/fullhttps://doaj.org/toc/2296-889XThe SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell entry mediators are expressed at low levels in placental cells, the receptors for viruses that cause congenital infections such as the cytomegalovirus and Zika virus are highly expressed in these cells. Here we analyzed the transcriptional profile (microarray and single-cell RNA-Seq) of proteins potentially interacting with coronaviruses to identify non- canonical mediators of SARS-CoV-2 infection and replication in the placenta. Despite low levels of the canonical cell entry mediators ACE2 and TMPRSS2, we show that cells of the syncytiotrophoblast, villous cytotrophoblast, and extravillous trophoblast co-express high levels of the potential non-canonical cell-entry mediators DPP4 and CTSL. We also found changes in the expression of DAAM1 and PAICS genes during pregnancy, which are translated into proteins also predicted to interact with coronaviruses proteins. These results provide new insight into the interaction between SARS-CoV-2 and host proteins that may act as non-canonical routes for SARS-CoV-2 infection and replication in the placenta cells.Flávia Bessi ConstantinoSarah Santiloni CuryCelia Regina NogueiraRobson Francisco CarvalhoLuis Antonio JustulinFrontiers Media S.A.articleCOVID-19SARS-CoV-2placentavirus entry mediatorDPP4CTSLBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 placenta virus entry mediator DPP4 CTSL Biology (General) QH301-705.5
spellingShingle	COVID-19 SARS-CoV-2 placenta virus entry mediator DPP4 CTSL Biology (General) QH301-705.5 Flávia Bessi Constantino Sarah Santiloni Cury Celia Regina Nogueira Robson Francisco Carvalho Luis Antonio Justulin Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
description	The SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell entry mediators are expressed at low levels in placental cells, the receptors for viruses that cause congenital infections such as the cytomegalovirus and Zika virus are highly expressed in these cells. Here we analyzed the transcriptional profile (microarray and single-cell RNA-Seq) of proteins potentially interacting with coronaviruses to identify non- canonical mediators of SARS-CoV-2 infection and replication in the placenta. Despite low levels of the canonical cell entry mediators ACE2 and TMPRSS2, we show that cells of the syncytiotrophoblast, villous cytotrophoblast, and extravillous trophoblast co-express high levels of the potential non-canonical cell-entry mediators DPP4 and CTSL. We also found changes in the expression of DAAM1 and PAICS genes during pregnancy, which are translated into proteins also predicted to interact with coronaviruses proteins. These results provide new insight into the interaction between SARS-CoV-2 and host proteins that may act as non-canonical routes for SARS-CoV-2 infection and replication in the placenta cells.
format	article
author	Flávia Bessi Constantino Sarah Santiloni Cury Celia Regina Nogueira Robson Francisco Carvalho Luis Antonio Justulin
author_facet	Flávia Bessi Constantino Sarah Santiloni Cury Celia Regina Nogueira Robson Francisco Carvalho Luis Antonio Justulin
author_sort	Flávia Bessi Constantino
title	Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_short	Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_full	Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_fullStr	Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_full_unstemmed	Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_sort	prediction of non-canonical routes for sars-cov-2 infection in human placenta cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d41b158c3d734d92bae4e18b8d8e1b44
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Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells

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