A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens

Abstract Quantification of iron is an important step to assess the iron burden in patients suffering from iron overload diseases, as well as tremendous value in understanding the underlying role of iron in the pathophysiology of these diseases. Current iron determination of total or labile iron, req...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Usama Abbasi, Srinivas Abbina, Arshdeep Gill, Vriti Bhagat, Jayachandran N. Kizhakkedathu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4248afcc30a4ec694a440329705d47a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d4248afcc30a4ec694a440329705d47a
record_format	dspace
spelling	oai:doaj.org-article:d4248afcc30a4ec694a440329705d47a2021-12-02T13:18:08ZA facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens10.1038/s41598-021-85387-z2045-2322https://doaj.org/article/d4248afcc30a4ec694a440329705d47a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85387-zhttps://doaj.org/toc/2045-2322Abstract Quantification of iron is an important step to assess the iron burden in patients suffering from iron overload diseases, as well as tremendous value in understanding the underlying role of iron in the pathophysiology of these diseases. Current iron determination of total or labile iron, requires extensive sample handling and specialized instruments, whilst being time consuming and laborious. Moreover, there is minimal to no overlap between total iron and labile iron quantification methodologies—i.e. requiring entirely separate protocols, techniques and instruments. Herein, we report a unified-ferene (u-ferene) assay that enables a 2-in-1 quantification of both labile and total iron from the same preparation of a biological specimen. We demonstrate that labile iron concentrations determined from the u-ferene assay is in agreement with confocal laser scanning microscopy techniques employed within the literature. Further, this assay offers the same sensitivity as the current gold standard, inductively coupled plasma mass spectrometry (ICP-MS), for total iron measurements. The new u-ferene assay will have tremendous value for the wider scientific community as it offers an economic and readily accessible method for convenient 2-in-1 measurement of total and labile iron from biological samples, whilst maintaining the precision and sensitivity, as compared to ICP-MS.Usama AbbasiSrinivas AbbinaArshdeep GillVriti BhagatJayachandran N. KizhakkedathuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Usama Abbasi Srinivas Abbina Arshdeep Gill Vriti Bhagat Jayachandran N. Kizhakkedathu A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
description	Abstract Quantification of iron is an important step to assess the iron burden in patients suffering from iron overload diseases, as well as tremendous value in understanding the underlying role of iron in the pathophysiology of these diseases. Current iron determination of total or labile iron, requires extensive sample handling and specialized instruments, whilst being time consuming and laborious. Moreover, there is minimal to no overlap between total iron and labile iron quantification methodologies—i.e. requiring entirely separate protocols, techniques and instruments. Herein, we report a unified-ferene (u-ferene) assay that enables a 2-in-1 quantification of both labile and total iron from the same preparation of a biological specimen. We demonstrate that labile iron concentrations determined from the u-ferene assay is in agreement with confocal laser scanning microscopy techniques employed within the literature. Further, this assay offers the same sensitivity as the current gold standard, inductively coupled plasma mass spectrometry (ICP-MS), for total iron measurements. The new u-ferene assay will have tremendous value for the wider scientific community as it offers an economic and readily accessible method for convenient 2-in-1 measurement of total and labile iron from biological samples, whilst maintaining the precision and sensitivity, as compared to ICP-MS.
format	article
author	Usama Abbasi Srinivas Abbina Arshdeep Gill Vriti Bhagat Jayachandran N. Kizhakkedathu
author_facet	Usama Abbasi Srinivas Abbina Arshdeep Gill Vriti Bhagat Jayachandran N. Kizhakkedathu
author_sort	Usama Abbasi
title	A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
title_short	A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
title_full	A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
title_fullStr	A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
title_full_unstemmed	A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
title_sort	facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d4248afcc30a4ec694a440329705d47a
work_keys_str_mv	AT usamaabbasi afacilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT srinivasabbina afacilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT arshdeepgill afacilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT vritibhagat afacilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT jayachandrannkizhakkedathu afacilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT usamaabbasi facilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT srinivasabbina facilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT arshdeepgill facilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT vritibhagat facilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens AT jayachandrannkizhakkedathu facilecolorimetricmethodforthequantificationoflabileironpoolandtotalironincellsandtissuespecimens
_version_	1718393297473372160

A facile colorimetric method for the quantification of labile iron pool and total iron in cells and tissue specimens

Ejemplares similares