Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model

Yi Zheng,1,* Jinbao Qin,2,* Xin Wang,2,* Zhiyou Peng,2 Peiyong Hou,1 Xinwu Lu2,3 1Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi, 2Department of Vascular Surgery, School of Medicine, Shanghai Ninth People’s Hospital Affiliated to Shangh...

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Autores principales:	Zheng Y, Qin JB, Wang X, Peng ZY, Hou PY, Lu XW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	allogeneic adipose derived stem cells (ADRCs) cell tracking APTS nanoparticles hindlimb ischemia ApoE knockout mouse Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d4275202df354dec93c1a7130fb1e974
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spelling	oai:doaj.org-article:d4275202df354dec93c1a7130fb1e9742021-12-02T02:28:07ZDynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model1178-2013https://doaj.org/article/d4275202df354dec93c1a7130fb1e9742016-12-01T00:00:00Zhttps://www.dovepress.com/dynamic-imaging-of-allogeneic-adipose-derived-regenerative-cells-trans-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yi Zheng,1,* Jinbao Qin,2,* Xin Wang,2,* Zhiyou Peng,2 Peiyong Hou,1 Xinwu Lu2,3 1Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi, 2Department of Vascular Surgery, School of Medicine, Shanghai Ninth People’s Hospital Affiliated to Shanghai JiaoTong University, 3Vascular Center of Shanghai JiaoTong University, Shanghai, People’s Republic of China*These authors contributed equally to this work Background: Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis.Materials and methods: In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs) were obtained, labeled with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs), and injected into an old apolipoprotein E knockout (ApoE-KO) mouse model with hind limb ischemia. Then, 3.0 T magnetic resonance imaging (MRI) was performed to dynamically trace the role of ADRCs targeting hind limb ischemia in the ApoE-KO mice model.Results: Labeled cells were visualized as large hypointense spots in ischemic muscles by serial 3.0 T MRI scans during a 4-week follow-up. The presence of labeled GFP-ADRCs was confirmed by Prussian blue staining and fluorescence microscopy on postmortem specimens.Conclusion: This study showed that allogeneic ADRCs offer great potential application for therapeutic angiogenesis in severe ischemic disease based on the efficacy and feasibility of ADRC transplantation and on the available amounts of tissue. Keywords: allogeneic adipose-derived stem cells, cell tracking, APTS nanoparticles, hind limb ischemia, ApoE knockout mouseZheng YQin JBWang XPeng ZYHou PYLu XWDove Medical Pressarticleallogeneic adipose derived stem cells (ADRCs)cell trackingAPTS nanoparticleshindlimb ischemiaApoE knockout mouseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 61-71 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	allogeneic adipose derived stem cells (ADRCs) cell tracking APTS nanoparticles hindlimb ischemia ApoE knockout mouse Medicine (General) R5-920
spellingShingle	allogeneic adipose derived stem cells (ADRCs) cell tracking APTS nanoparticles hindlimb ischemia ApoE knockout mouse Medicine (General) R5-920 Zheng Y Qin JB Wang X Peng ZY Hou PY Lu XW Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
description	Yi Zheng,1,* Jinbao Qin,2,* Xin Wang,2,* Zhiyou Peng,2 Peiyong Hou,1 Xinwu Lu2,3 1Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi, 2Department of Vascular Surgery, School of Medicine, Shanghai Ninth People’s Hospital Affiliated to Shanghai JiaoTong University, 3Vascular Center of Shanghai JiaoTong University, Shanghai, People’s Republic of China*These authors contributed equally to this work Background: Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis.Materials and methods: In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs) were obtained, labeled with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs), and injected into an old apolipoprotein E knockout (ApoE-KO) mouse model with hind limb ischemia. Then, 3.0 T magnetic resonance imaging (MRI) was performed to dynamically trace the role of ADRCs targeting hind limb ischemia in the ApoE-KO mice model.Results: Labeled cells were visualized as large hypointense spots in ischemic muscles by serial 3.0 T MRI scans during a 4-week follow-up. The presence of labeled GFP-ADRCs was confirmed by Prussian blue staining and fluorescence microscopy on postmortem specimens.Conclusion: This study showed that allogeneic ADRCs offer great potential application for therapeutic angiogenesis in severe ischemic disease based on the efficacy and feasibility of ADRC transplantation and on the available amounts of tissue. Keywords: allogeneic adipose-derived stem cells, cell tracking, APTS nanoparticles, hind limb ischemia, ApoE knockout mouse
format	article
author	Zheng Y Qin JB Wang X Peng ZY Hou PY Lu XW
author_facet	Zheng Y Qin JB Wang X Peng ZY Hou PY Lu XW
author_sort	Zheng Y
title	Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
title_short	Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
title_full	Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
title_fullStr	Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
title_full_unstemmed	Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
title_sort	dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein e mouse model
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/d4275202df354dec93c1a7130fb1e974
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Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model

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