The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway

Apigenin, a common dietary flavonoid abundantly present in a variety of fruits and vegetables, has promising anticancer properties. As an effector of apigenin in myoblasts, protein arginine methyltransferase 7 (Prmt7) is required for male germ cell development. However, whether ap...

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Autores principales:	Bingyuan Wang, Mingrui Zhang, Jiankang Guo, Zhiguo Liu, Rong Zhou, Fei Guo, Kui Li, Yulian Mu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apigenin spermatogonial proliferation <i>Prmt7</i> <i>Akt3</i> mouse Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d435b2e721964d0f99bd1a340da5a1b4
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spelling	oai:doaj.org-article:d435b2e721964d0f99bd1a340da5a1b42021-11-25T17:54:21ZThe Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway10.3390/ijms2222122091422-00671661-6596https://doaj.org/article/d435b2e721964d0f99bd1a340da5a1b42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12209https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Apigenin, a common dietary flavonoid abundantly present in a variety of fruits and vegetables, has promising anticancer properties. As an effector of apigenin in myoblasts, protein arginine methyltransferase 7 (<i>Prmt7</i>) is required for male germ cell development. However, whether apigenin may influence male reproductive health through <i>Prmt7</i> is still unclear. To this end, mouse spermatogonia were treated with different concentrations (2.5 to 50 μM) of apigenin for 48 h, which showed that apigenin could cause reduced cell proliferation in conjunction with longer S phase and G2/M phase (with concentrations of 10 and 20 μM, respectively), and increased apoptosis of spermatogonia (with concentration of 20 μM). Reduced <i>Prmt7</i> expression was found in 20 μM apigenin-treated spermatogonia. Moreover, siRNA-induced <i>Prmt7</i> knockdown exhibited similar influence on spermatogonia as that of apigenin treatment. In mechanistic terms, transcriptome analysis revealed 287 differentially expressed genes between <i>Prmt7</i>-downregulated and control spermatogonia. Furthermore, rescue experiments suggested that the effects of apigenin on spermatogonia might be mediated through the <i>Prmt7</i>/<i>Akt3</i> pathway. Overall, our study supports that apigenin can interfere with mouse spermatogonial proliferation by way of the downregulated <i>Prmt7</i>/<i>Akt3</i> pathway, which demonstrates that the concentration should be taken into account in future applications of apigenin for cancer therapy of men.Bingyuan WangMingrui ZhangJiankang GuoZhiguo LiuRong ZhouFei GuoKui LiYulian MuMDPI AGarticleapigeninspermatogonial proliferation<i>Prmt7</i><i>Akt3</i>mouseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12209, p 12209 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apigenin spermatogonial proliferation <i>Prmt7</i> <i>Akt3</i> mouse Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	apigenin spermatogonial proliferation <i>Prmt7</i> <i>Akt3</i> mouse Biology (General) QH301-705.5 Chemistry QD1-999 Bingyuan Wang Mingrui Zhang Jiankang Guo Zhiguo Liu Rong Zhou Fei Guo Kui Li Yulian Mu The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
description	Apigenin, a common dietary flavonoid abundantly present in a variety of fruits and vegetables, has promising anticancer properties. As an effector of apigenin in myoblasts, protein arginine methyltransferase 7 (<i>Prmt7</i>) is required for male germ cell development. However, whether apigenin may influence male reproductive health through <i>Prmt7</i> is still unclear. To this end, mouse spermatogonia were treated with different concentrations (2.5 to 50 μM) of apigenin for 48 h, which showed that apigenin could cause reduced cell proliferation in conjunction with longer S phase and G2/M phase (with concentrations of 10 and 20 μM, respectively), and increased apoptosis of spermatogonia (with concentration of 20 μM). Reduced <i>Prmt7</i> expression was found in 20 μM apigenin-treated spermatogonia. Moreover, siRNA-induced <i>Prmt7</i> knockdown exhibited similar influence on spermatogonia as that of apigenin treatment. In mechanistic terms, transcriptome analysis revealed 287 differentially expressed genes between <i>Prmt7</i>-downregulated and control spermatogonia. Furthermore, rescue experiments suggested that the effects of apigenin on spermatogonia might be mediated through the <i>Prmt7</i>/<i>Akt3</i> pathway. Overall, our study supports that apigenin can interfere with mouse spermatogonial proliferation by way of the downregulated <i>Prmt7</i>/<i>Akt3</i> pathway, which demonstrates that the concentration should be taken into account in future applications of apigenin for cancer therapy of men.
format	article
author	Bingyuan Wang Mingrui Zhang Jiankang Guo Zhiguo Liu Rong Zhou Fei Guo Kui Li Yulian Mu
author_facet	Bingyuan Wang Mingrui Zhang Jiankang Guo Zhiguo Liu Rong Zhou Fei Guo Kui Li Yulian Mu
author_sort	Bingyuan Wang
title	The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
title_short	The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
title_full	The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
title_fullStr	The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
title_full_unstemmed	The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway
title_sort	effects of flavonoid apigenin on male reproductive health: inhibition of spermatogonial proliferation through downregulation of <em>prmt7</em>/<em>akt3</em> pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d435b2e721964d0f99bd1a340da5a1b4
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The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of <em>Prmt7</em>/<em>Akt3</em> Pathway

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