Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy

Background. Celiac disease (CeD) is an autoimmune intestinal disorder caused by gluten protein consumption in genetically predisposed individuals. As biopsy sampling is an invasive procedure, finding novel noninvasive serological markers for screening of at-risk CeD population is a priority. Metabol...

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Autores principales:	Ensieh Khalkhal, Mostafa Rezaei-Tavirani, Fariba Fathi, B. Fatemeh Nobakht M. Gh, Amir Taherkhani, Mohammad Rostami-Nejad, Nastaran Asri, Mohammad Hossain Haidari
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/d449c61c0a834634a9281bd26b26327a
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spelling	oai:doaj.org-article:d449c61c0a834634a9281bd26b26327a2021-11-29T00:55:47ZScreening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy2314-614110.1155/2021/1798783https://doaj.org/article/d449c61c0a834634a9281bd26b26327a2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1798783https://doaj.org/toc/2314-6141Background. Celiac disease (CeD) is an autoimmune intestinal disorder caused by gluten protein consumption in genetically predisposed individuals. As biopsy sampling is an invasive procedure, finding novel noninvasive serological markers for screening of at-risk CeD population is a priority. Metabolomics is helpful in monitoring metabolite changes in body fluids and tissues. In the present study, we evaluated serum metabolite levels of CeD patients relative to healthy controls with the aim of introducing new biomarkers for population screening. Method. We compared the serum metabolic profile of CeD patients (n=42) and healthy controls (n=22) using NMR spectroscopy and multivariate analysis. Result. 25 metabolites were identified by serum metabolic profiling. Levels of 3-hydroxyisobutyric acid and isobutyrate showed significant differences in CeD patients’ samples compared with healthy controls (p<0.05). According to pathway analysis, our data demonstrated that changes in nine metabolic pathways were significantly disrupted/affected in patients with CeD. These enriched pathways are involved in aminoacyl-tRNA biosynthesis; primary bile acid biosynthesis; nitrogen metabolism; glutamine and glutamate metabolism; valine, leucine, and isoleucine biosynthesis and degradation; taurine and hypotaurine metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; and arginine biosynthesis. Conclusion. In summary, our results demonstrated that changes in the serum level of 25 metabolites may be useful in distinguishing CeD patients from healthy controls, which have the potential to be considered candidate biomarkers of CeD.Ensieh KhalkhalMostafa Rezaei-TaviraniFariba FathiB. Fatemeh Nobakht M. GhAmir TaherkhaniMohammad Rostami-NejadNastaran AsriMohammad Hossain HaidariHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Ensieh Khalkhal Mostafa Rezaei-Tavirani Fariba Fathi B. Fatemeh Nobakht M. Gh Amir Taherkhani Mohammad Rostami-Nejad Nastaran Asri Mohammad Hossain Haidari Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
description	Background. Celiac disease (CeD) is an autoimmune intestinal disorder caused by gluten protein consumption in genetically predisposed individuals. As biopsy sampling is an invasive procedure, finding novel noninvasive serological markers for screening of at-risk CeD population is a priority. Metabolomics is helpful in monitoring metabolite changes in body fluids and tissues. In the present study, we evaluated serum metabolite levels of CeD patients relative to healthy controls with the aim of introducing new biomarkers for population screening. Method. We compared the serum metabolic profile of CeD patients (n=42) and healthy controls (n=22) using NMR spectroscopy and multivariate analysis. Result. 25 metabolites were identified by serum metabolic profiling. Levels of 3-hydroxyisobutyric acid and isobutyrate showed significant differences in CeD patients’ samples compared with healthy controls (p<0.05). According to pathway analysis, our data demonstrated that changes in nine metabolic pathways were significantly disrupted/affected in patients with CeD. These enriched pathways are involved in aminoacyl-tRNA biosynthesis; primary bile acid biosynthesis; nitrogen metabolism; glutamine and glutamate metabolism; valine, leucine, and isoleucine biosynthesis and degradation; taurine and hypotaurine metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; and arginine biosynthesis. Conclusion. In summary, our results demonstrated that changes in the serum level of 25 metabolites may be useful in distinguishing CeD patients from healthy controls, which have the potential to be considered candidate biomarkers of CeD.
format	article
author	Ensieh Khalkhal Mostafa Rezaei-Tavirani Fariba Fathi B. Fatemeh Nobakht M. Gh Amir Taherkhani Mohammad Rostami-Nejad Nastaran Asri Mohammad Hossain Haidari
author_facet	Ensieh Khalkhal Mostafa Rezaei-Tavirani Fariba Fathi B. Fatemeh Nobakht M. Gh Amir Taherkhani Mohammad Rostami-Nejad Nastaran Asri Mohammad Hossain Haidari
author_sort	Ensieh Khalkhal
title	Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
title_short	Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
title_full	Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
title_fullStr	Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
title_full_unstemmed	Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy
title_sort	screening of altered metabolites and metabolic pathways in celiac disease using nmr spectroscopy
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/d449c61c0a834634a9281bd26b26327a
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Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy

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