The chromosomal passenger complex activates Polo kinase at centromeres.

The coordinated activities at centromeres of two key cell cycle kinases, Polo and Aurora B, are critical for ensuring that the two sister kinetochores of each chromosome are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphase. Initial attachments of chrom...

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Autores principales:	Mar Carmena, Xavier Pinson, Melpi Platani, Zeina Salloum, Zhenjie Xu, Anthony Clark, Fiona Macisaac, Hiromi Ogawa, Ulrike Eggert, David M Glover, Vincent Archambault, William C Earnshaw
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d44a914551df4c03a4a1351d1a6845ce
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spelling	oai:doaj.org-article:d44a914551df4c03a4a1351d1a6845ce2021-11-18T05:36:47ZThe chromosomal passenger complex activates Polo kinase at centromeres.1544-91731545-788510.1371/journal.pbio.1001250https://doaj.org/article/d44a914551df4c03a4a1351d1a6845ce2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22291575/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The coordinated activities at centromeres of two key cell cycle kinases, Polo and Aurora B, are critical for ensuring that the two sister kinetochores of each chromosome are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphase. Initial attachments of chromosomes to the spindle involve random interactions between kinetochores and dynamic microtubules, and errors occur frequently during early stages of the process. The balance between microtubule binding and error correction (e.g., release of bound microtubules) requires the activities of Polo and Aurora B kinases, with Polo promoting stable attachments and Aurora B promoting detachment. Our study concerns the coordination of the activities of these two kinases in vivo. We show that INCENP, a key scaffolding subunit of the chromosomal passenger complex (CPC), which consists of Aurora B kinase, INCENP, Survivin, and Borealin/Dasra B, also interacts with Polo kinase in Drosophila cells. It was known that Aurora A/Bora activates Polo at centrosomes during late G2. However, the kinase that activates Polo on chromosomes for its critical functions at kinetochores was not known. We show here that Aurora B kinase phosphorylates Polo on its activation loop at the centromere in early mitosis. This phosphorylation requires both INCENP and Aurora B activity (but not Aurora A activity) and is critical for Polo function at kinetochores. Our results demonstrate clearly that Polo kinase is regulated differently at centrosomes and centromeres and suggest that INCENP acts as a platform for kinase crosstalk at the centromere. This crosstalk may enable Polo and Aurora B to achieve a balance wherein microtubule mis-attachments are corrected, but proper attachments are stabilized allowing proper chromosome segregation.Mar CarmenaXavier PinsonMelpi PlataniZeina SalloumZhenjie XuAnthony ClarkFiona MacisaacHiromi OgawaUlrike EggertDavid M GloverVincent ArchambaultWilliam C EarnshawPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 10, Iss 1, p e1001250 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Mar Carmena Xavier Pinson Melpi Platani Zeina Salloum Zhenjie Xu Anthony Clark Fiona Macisaac Hiromi Ogawa Ulrike Eggert David M Glover Vincent Archambault William C Earnshaw The chromosomal passenger complex activates Polo kinase at centromeres.
description	The coordinated activities at centromeres of two key cell cycle kinases, Polo and Aurora B, are critical for ensuring that the two sister kinetochores of each chromosome are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphase. Initial attachments of chromosomes to the spindle involve random interactions between kinetochores and dynamic microtubules, and errors occur frequently during early stages of the process. The balance between microtubule binding and error correction (e.g., release of bound microtubules) requires the activities of Polo and Aurora B kinases, with Polo promoting stable attachments and Aurora B promoting detachment. Our study concerns the coordination of the activities of these two kinases in vivo. We show that INCENP, a key scaffolding subunit of the chromosomal passenger complex (CPC), which consists of Aurora B kinase, INCENP, Survivin, and Borealin/Dasra B, also interacts with Polo kinase in Drosophila cells. It was known that Aurora A/Bora activates Polo at centrosomes during late G2. However, the kinase that activates Polo on chromosomes for its critical functions at kinetochores was not known. We show here that Aurora B kinase phosphorylates Polo on its activation loop at the centromere in early mitosis. This phosphorylation requires both INCENP and Aurora B activity (but not Aurora A activity) and is critical for Polo function at kinetochores. Our results demonstrate clearly that Polo kinase is regulated differently at centrosomes and centromeres and suggest that INCENP acts as a platform for kinase crosstalk at the centromere. This crosstalk may enable Polo and Aurora B to achieve a balance wherein microtubule mis-attachments are corrected, but proper attachments are stabilized allowing proper chromosome segregation.
format	article
author	Mar Carmena Xavier Pinson Melpi Platani Zeina Salloum Zhenjie Xu Anthony Clark Fiona Macisaac Hiromi Ogawa Ulrike Eggert David M Glover Vincent Archambault William C Earnshaw
author_facet	Mar Carmena Xavier Pinson Melpi Platani Zeina Salloum Zhenjie Xu Anthony Clark Fiona Macisaac Hiromi Ogawa Ulrike Eggert David M Glover Vincent Archambault William C Earnshaw
author_sort	Mar Carmena
title	The chromosomal passenger complex activates Polo kinase at centromeres.
title_short	The chromosomal passenger complex activates Polo kinase at centromeres.
title_full	The chromosomal passenger complex activates Polo kinase at centromeres.
title_fullStr	The chromosomal passenger complex activates Polo kinase at centromeres.
title_full_unstemmed	The chromosomal passenger complex activates Polo kinase at centromeres.
title_sort	chromosomal passenger complex activates polo kinase at centromeres.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d44a914551df4c03a4a1351d1a6845ce
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The chromosomal passenger complex activates Polo kinase at centromeres.

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