Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer

Gold nanoparticles (AuNPs) were fabricated with biocompatible collagen (Col) and then conjugated with berberine (BB), denoted as Au-Col-BB, to investigate the endocytic mechanisms in Her-2 breast cancer cell line and in bovine aortic endothelial cells (BAEC). Owing to the superior biocompatibility,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen-Feng Chiu, Ru-Huei Fu, Shan-hui Hsu, Yang-Hao (Alex) Yu, Shun-Fa Yang, Thomas Chang-Yao Tsao, Kai-Bo Chang, Chun-An Yeh, Cheng-Ming Tang, Sheng-Chu Huang, Huey-Shan Hung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	berberine collagen gold nanoparticles breast cancer endocytosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d44c17088d28454ba2a0cf5c8d030b07
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d44c17088d28454ba2a0cf5c8d030b07
record_format	dspace
spelling	oai:doaj.org-article:d44c17088d28454ba2a0cf5c8d030b072021-11-11T15:28:13ZDelivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer10.3390/cancers132153172072-6694https://doaj.org/article/d44c17088d28454ba2a0cf5c8d030b072021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5317https://doaj.org/toc/2072-6694Gold nanoparticles (AuNPs) were fabricated with biocompatible collagen (Col) and then conjugated with berberine (BB), denoted as Au-Col-BB, to investigate the endocytic mechanisms in Her-2 breast cancer cell line and in bovine aortic endothelial cells (BAEC). Owing to the superior biocompatibility, tunable physicochemical properties, and potential functionalization with biomolecules, AuNPs have been well studied as carriers of biomolecules for diseases and cancer therapeutics. Composites of AuNPs with biopolymer, such as fibronectin or Col, have been revealed to increase cell proliferation, migration, and differentiation. BB is a natural compound with impressive health benefits, such as lowering blood sugar and reducing weight. In addition, BB can inhibit cell proliferation by modulating cell cycle progress and autophagy, and induce cell apoptosis in vivo and in vitro. In the current research, BB was conjugated on the Col-AuNP composite (“Au-Col”). The UV-Visible spectroscopy and infrared spectroscopy confirmed the conjugation of BB on Au-Col. The particle size of the Au-Col-BB conjugate was about 227 nm, determined by dynamic light scattering. Furthermore, Au-Col-BB was less cytotoxic to BAEC vs. Her-2 cell line in terms of MTT assay and cell cycle behavior. Au-Col-BB, compared to Au-Col, showed greater cell uptake capacity and potential cellular transportation by BAEC and Her-2 using the fluorescence-conjugated Au-Col-BB. In addition, the clathrin-mediated endocytosis and cell autophagy seemed to be the favorite endocytic mechanism for the internalization of Au-Col-BB by BAEC and Her-2. Au-Col-BB significantly inhibited cell migration in Her-2, but not in BAEC. Moreover, apoptotic cascade proteins, such as Bax and p21, were expressed in Her-2 after the treatment of Au-Col-BB. The tumor suppression was examined in a model of xenograft mice treated with Au-Col-BB nanovehicles. Results demonstrated that the tumor weight was remarkably reduced by the treatment of Au-Col-BB. Altogether, the promising findings of Au-Col-BB nanocarrier on Her-2 breast cancer cell line suggest that Au-Col-BB may be a good candidate of anticancer drug for the treatment of human breast cancer.Chen-Feng ChiuRu-Huei FuShan-hui HsuYang-Hao (Alex) YuShun-Fa YangThomas Chang-Yao TsaoKai-Bo ChangChun-An YehCheng-Ming TangSheng-Chu HuangHuey-Shan HungMDPI AGarticleberberinecollagengold nanoparticlesbreast cancerendocytosisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5317, p 5317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	berberine collagen gold nanoparticles breast cancer endocytosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	berberine collagen gold nanoparticles breast cancer endocytosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Chen-Feng Chiu Ru-Huei Fu Shan-hui Hsu Yang-Hao (Alex) Yu Shun-Fa Yang Thomas Chang-Yao Tsao Kai-Bo Chang Chun-An Yeh Cheng-Ming Tang Sheng-Chu Huang Huey-Shan Hung Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
description	Gold nanoparticles (AuNPs) were fabricated with biocompatible collagen (Col) and then conjugated with berberine (BB), denoted as Au-Col-BB, to investigate the endocytic mechanisms in Her-2 breast cancer cell line and in bovine aortic endothelial cells (BAEC). Owing to the superior biocompatibility, tunable physicochemical properties, and potential functionalization with biomolecules, AuNPs have been well studied as carriers of biomolecules for diseases and cancer therapeutics. Composites of AuNPs with biopolymer, such as fibronectin or Col, have been revealed to increase cell proliferation, migration, and differentiation. BB is a natural compound with impressive health benefits, such as lowering blood sugar and reducing weight. In addition, BB can inhibit cell proliferation by modulating cell cycle progress and autophagy, and induce cell apoptosis in vivo and in vitro. In the current research, BB was conjugated on the Col-AuNP composite (“Au-Col”). The UV-Visible spectroscopy and infrared spectroscopy confirmed the conjugation of BB on Au-Col. The particle size of the Au-Col-BB conjugate was about 227 nm, determined by dynamic light scattering. Furthermore, Au-Col-BB was less cytotoxic to BAEC vs. Her-2 cell line in terms of MTT assay and cell cycle behavior. Au-Col-BB, compared to Au-Col, showed greater cell uptake capacity and potential cellular transportation by BAEC and Her-2 using the fluorescence-conjugated Au-Col-BB. In addition, the clathrin-mediated endocytosis and cell autophagy seemed to be the favorite endocytic mechanism for the internalization of Au-Col-BB by BAEC and Her-2. Au-Col-BB significantly inhibited cell migration in Her-2, but not in BAEC. Moreover, apoptotic cascade proteins, such as Bax and p21, were expressed in Her-2 after the treatment of Au-Col-BB. The tumor suppression was examined in a model of xenograft mice treated with Au-Col-BB nanovehicles. Results demonstrated that the tumor weight was remarkably reduced by the treatment of Au-Col-BB. Altogether, the promising findings of Au-Col-BB nanocarrier on Her-2 breast cancer cell line suggest that Au-Col-BB may be a good candidate of anticancer drug for the treatment of human breast cancer.
format	article
author	Chen-Feng Chiu Ru-Huei Fu Shan-hui Hsu Yang-Hao (Alex) Yu Shun-Fa Yang Thomas Chang-Yao Tsao Kai-Bo Chang Chun-An Yeh Cheng-Ming Tang Sheng-Chu Huang Huey-Shan Hung
author_facet	Chen-Feng Chiu Ru-Huei Fu Shan-hui Hsu Yang-Hao (Alex) Yu Shun-Fa Yang Thomas Chang-Yao Tsao Kai-Bo Chang Chun-An Yeh Cheng-Ming Tang Sheng-Chu Huang Huey-Shan Hung
author_sort	Chen-Feng Chiu
title	Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
title_short	Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
title_full	Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
title_fullStr	Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
title_full_unstemmed	Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
title_sort	delivery capacity and anticancer ability of the berberine-loaded gold nanoparticles to promote the apoptosis effect in breast cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d44c17088d28454ba2a0cf5c8d030b07
work_keys_str_mv	AT chenfengchiu deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT ruhueifu deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT shanhuihsu deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT yanghaoalexyu deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT shunfayang deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT thomaschangyaotsao deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT kaibochang deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT chunanyeh deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT chengmingtang deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT shengchuhuang deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer AT hueyshanhung deliverycapacityandanticancerabilityoftheberberineloadedgoldnanoparticlestopromotetheapoptosiseffectinbreastcancer
_version_	1718435272437268480

Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer

Ejemplares similares