Boron stress responsive microRNAs and their targets in barley.

Boron stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. In this study, miRNAs associated with boron stress were identified and characteri...

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Autores principales:	Esma Ozhuner, Vahap Eldem, Arif Ipek, Sezer Okay, Serdal Sakcali, Baohong Zhang, Hatice Boke, Turgay Unver
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d44c2e325b764bbead6b139472fd4408
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spelling	oai:doaj.org-article:d44c2e325b764bbead6b139472fd44082021-11-18T07:52:00ZBoron stress responsive microRNAs and their targets in barley.1932-620310.1371/journal.pone.0059543https://doaj.org/article/d44c2e325b764bbead6b139472fd44082013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555702/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Boron stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. In this study, miRNAs associated with boron stress were identified and characterized in barley. miRNA profiles were also comparatively analyzed between root and leave samples. A total of 31 known and 3 new miRNAs were identified in barley; 25 of them were found to respond to boron treatment. Several miRNAs were expressed in a tissue specific manner; for example, miR156d, miR171a, miR397, and miR444a were only detected in leaves. Additionally, a total of 934 barley transcripts were found to be specifically targeted and degraded by miRNAs. In silico analysis of miRNA target genes demonstrated that many miRNA targets are conserved transcription factors such as Squamosa promoter-binding protein, Auxin response factor (ARF), and the MYB transcription factor family. A majority of these targets were responsible for plant growth and response to environmental changes. We also propose that some of the miRNAs in barley such as miRNA408 might play critical roles against boron exposure. In conclusion, barley may use several pathways and cellular processes targeted by miRNAs to cope with boron stress.Esma OzhunerVahap EldemArif IpekSezer OkaySerdal SakcaliBaohong ZhangHatice BokeTurgay UnverPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59543 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Esma Ozhuner Vahap Eldem Arif Ipek Sezer Okay Serdal Sakcali Baohong Zhang Hatice Boke Turgay Unver Boron stress responsive microRNAs and their targets in barley.
description	Boron stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. In this study, miRNAs associated with boron stress were identified and characterized in barley. miRNA profiles were also comparatively analyzed between root and leave samples. A total of 31 known and 3 new miRNAs were identified in barley; 25 of them were found to respond to boron treatment. Several miRNAs were expressed in a tissue specific manner; for example, miR156d, miR171a, miR397, and miR444a were only detected in leaves. Additionally, a total of 934 barley transcripts were found to be specifically targeted and degraded by miRNAs. In silico analysis of miRNA target genes demonstrated that many miRNA targets are conserved transcription factors such as Squamosa promoter-binding protein, Auxin response factor (ARF), and the MYB transcription factor family. A majority of these targets were responsible for plant growth and response to environmental changes. We also propose that some of the miRNAs in barley such as miRNA408 might play critical roles against boron exposure. In conclusion, barley may use several pathways and cellular processes targeted by miRNAs to cope with boron stress.
format	article
author	Esma Ozhuner Vahap Eldem Arif Ipek Sezer Okay Serdal Sakcali Baohong Zhang Hatice Boke Turgay Unver
author_facet	Esma Ozhuner Vahap Eldem Arif Ipek Sezer Okay Serdal Sakcali Baohong Zhang Hatice Boke Turgay Unver
author_sort	Esma Ozhuner
title	Boron stress responsive microRNAs and their targets in barley.
title_short	Boron stress responsive microRNAs and their targets in barley.
title_full	Boron stress responsive microRNAs and their targets in barley.
title_fullStr	Boron stress responsive microRNAs and their targets in barley.
title_full_unstemmed	Boron stress responsive microRNAs and their targets in barley.
title_sort	boron stress responsive micrornas and their targets in barley.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d44c2e325b764bbead6b139472fd4408
work_keys_str_mv	AT esmaozhuner boronstressresponsivemicrornasandtheirtargetsinbarley AT vahapeldem boronstressresponsivemicrornasandtheirtargetsinbarley AT arifipek boronstressresponsivemicrornasandtheirtargetsinbarley AT sezerokay boronstressresponsivemicrornasandtheirtargetsinbarley AT serdalsakcali boronstressresponsivemicrornasandtheirtargetsinbarley AT baohongzhang boronstressresponsivemicrornasandtheirtargetsinbarley AT haticeboke boronstressresponsivemicrornasandtheirtargetsinbarley AT turgayunver boronstressresponsivemicrornasandtheirtargetsinbarley
_version_	1718422881654800384

Boron stress responsive microRNAs and their targets in barley.

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