Inactivated vaccine against Aujeszky's disease

Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on...

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Autores principales:	Zhanat B. Kondibaeva, Bolat A. Yespembetov, Khairulla B. Abeuov, Assiya K. Mussayeva, Sarsenbek T. Siyabekov, Saltanat T. Nussupova, Elmira K. Akmatova, Yerlan K. Pazylov, Kydyrbay T. Maikhin, Nazym S. Syrym
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	adjuvant aujeszky's disease virus colostral immunity immunogenicity strain "kordai" Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d44e9a47b5f849f1ac97587bc5aa32bf
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spelling	oai:doaj.org-article:d44e9a47b5f849f1ac97587bc5aa32bf2021-11-24T13:34:10ZInactivated vaccine against Aujeszky's disease10.14202/vetworld.2021.2957-29630972-89882231-0916https://doaj.org/article/d44e9a47b5f849f1ac97587bc5aa32bf2021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/16.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain. Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%. Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells). Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.Zhanat B. KondibaevaBolat A. YespembetovKhairulla B. AbeuovAssiya K. MussayevaSarsenbek T. SiyabekovSaltanat T. NussupovaElmira K. AkmatovaYerlan K. PazylovKydyrbay T. MaikhinNazym S. SyrymVeterinary Worldarticleadjuvantaujeszky's disease viruscolostral immunityimmunogenicitystrain "kordai"Animal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 2957-2963 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adjuvant aujeszky's disease virus colostral immunity immunogenicity strain "kordai" Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	adjuvant aujeszky's disease virus colostral immunity immunogenicity strain "kordai" Animal culture SF1-1100 Veterinary medicine SF600-1100 Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym Inactivated vaccine against Aujeszky's disease
description	Background and Aim: The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain. Materials and Methods: To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were used in concentrations of 0.1%, 0.08%, and 0.04%. Results: As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells). Conclusion: To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.
format	article
author	Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym
author_facet	Zhanat B. Kondibaeva Bolat A. Yespembetov Khairulla B. Abeuov Assiya K. Mussayeva Sarsenbek T. Siyabekov Saltanat T. Nussupova Elmira K. Akmatova Yerlan K. Pazylov Kydyrbay T. Maikhin Nazym S. Syrym
author_sort	Zhanat B. Kondibaeva
title	Inactivated vaccine against Aujeszky's disease
title_short	Inactivated vaccine against Aujeszky's disease
title_full	Inactivated vaccine against Aujeszky's disease
title_fullStr	Inactivated vaccine against Aujeszky's disease
title_full_unstemmed	Inactivated vaccine against Aujeszky's disease
title_sort	inactivated vaccine against aujeszky's disease
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/d44e9a47b5f849f1ac97587bc5aa32bf
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Inactivated vaccine against Aujeszky's disease

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