Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation

Abstract Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (...

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Autores principales:	Zuzana Malá, Ludmila Žárská, Lukáš Malina, Kateřina Langová, Renata Večeřová, Milan Kolář, Petr Henke, Jiří Mosinger, Hana Kolářová
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d450835664094fce98a6e76861dceeb9
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spelling	oai:doaj.org-article:d450835664094fce98a6e76861dceeb92021-12-02T14:02:54ZPhotodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation10.1038/s41598-021-85828-92045-2322https://doaj.org/article/d450835664094fce98a6e76861dceeb92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85828-9https://doaj.org/toc/2045-2322Abstract Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.Zuzana MaláLudmila ŽárskáLukáš MalinaKateřina LangováRenata VečeřováMilan KolářPetr HenkeJiří MosingerHana KolářováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuzana Malá Ludmila Žárská Lukáš Malina Kateřina Langová Renata Večeřová Milan Kolář Petr Henke Jiří Mosinger Hana Kolářová Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
description	Abstract Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.
format	article
author	Zuzana Malá Ludmila Žárská Lukáš Malina Kateřina Langová Renata Večeřová Milan Kolář Petr Henke Jiří Mosinger Hana Kolářová
author_facet	Zuzana Malá Ludmila Žárská Lukáš Malina Kateřina Langová Renata Večeřová Milan Kolář Petr Henke Jiří Mosinger Hana Kolářová
author_sort	Zuzana Malá
title	Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
title_short	Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
title_full	Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
title_fullStr	Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
title_full_unstemmed	Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation
title_sort	photodynamic effect of tpp encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: afm evaluation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d450835664094fce98a6e76861dceeb9
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Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation

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