Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals

Abstract Plant metal hyperaccumulators, to which Brassica juncea belongs, must have very efficient defence mechanisms that enable growth and development in an environment polluted with various heavy metals. B. juncea (Indiana mustard) v. Małopolska was exposed to the activity of trace elements such...

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Autores principales:	Arleta Małecka, Agnieszka Konkolewska, Anetta Hanć, Liliana Ciszewska, Aleksandra Maria Staszak, Wieslawa Jarmuszkiewicz, Ewelina Ratajczak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d452f4b1ba22436ab3fca54ee485bdba
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spelling	oai:doaj.org-article:d452f4b1ba22436ab3fca54ee485bdba2021-11-21T12:18:09ZActivation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals10.1038/s41598-021-01827-w2045-2322https://doaj.org/article/d452f4b1ba22436ab3fca54ee485bdba2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01827-whttps://doaj.org/toc/2045-2322Abstract Plant metal hyperaccumulators, to which Brassica juncea belongs, must have very efficient defence mechanisms that enable growth and development in an environment polluted with various heavy metals. B. juncea (Indiana mustard) v. Małopolska was exposed to the activity of trace elements such as cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) in combinations: CuPb, CuCd, CuZn, PbCd, PbZn, and ZnCd in a concentration of 25 μM each for 96 h during control cultivation. We observed a clear tendency for metal uptake and accumulation in above-ground parts which is characteristic of hyperaccumulators. The combinations of CuCd, CuZn, and PbCd inhibited the development of the seedlings the most. The used metal combinations increased the levels of reactive oxygen species (ROS) such as: hydrogen peroxide (H2O2), superoxide anion (O2 .−) and oxidized proteins in B. juncea organs, generating oxidative stress conditions in the cells. We determined the level of transcription of the respective defence proteins of the detoxification and antioxidant systems. We have shown that in the first 24 h of stress condiction, activation of glutamylcysteine-γ synthetase (yECS) and glutathione reductase (GR1) enzymes related to the detoxification of heavy metals is important for B. juncea plants. In addition, the data provide important information on how plants respond to the presence of heavy metals in the first days of stress conditions.Arleta MałeckaAgnieszka KonkolewskaAnetta HanćLiliana CiszewskaAleksandra Maria StaszakWieslawa JarmuszkiewiczEwelina RatajczakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arleta Małecka Agnieszka Konkolewska Anetta Hanć Liliana Ciszewska Aleksandra Maria Staszak Wieslawa Jarmuszkiewicz Ewelina Ratajczak Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
description	Abstract Plant metal hyperaccumulators, to which Brassica juncea belongs, must have very efficient defence mechanisms that enable growth and development in an environment polluted with various heavy metals. B. juncea (Indiana mustard) v. Małopolska was exposed to the activity of trace elements such as cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) in combinations: CuPb, CuCd, CuZn, PbCd, PbZn, and ZnCd in a concentration of 25 μM each for 96 h during control cultivation. We observed a clear tendency for metal uptake and accumulation in above-ground parts which is characteristic of hyperaccumulators. The combinations of CuCd, CuZn, and PbCd inhibited the development of the seedlings the most. The used metal combinations increased the levels of reactive oxygen species (ROS) such as: hydrogen peroxide (H2O2), superoxide anion (O2 .−) and oxidized proteins in B. juncea organs, generating oxidative stress conditions in the cells. We determined the level of transcription of the respective defence proteins of the detoxification and antioxidant systems. We have shown that in the first 24 h of stress condiction, activation of glutamylcysteine-γ synthetase (yECS) and glutathione reductase (GR1) enzymes related to the detoxification of heavy metals is important for B. juncea plants. In addition, the data provide important information on how plants respond to the presence of heavy metals in the first days of stress conditions.
format	article
author	Arleta Małecka Agnieszka Konkolewska Anetta Hanć Liliana Ciszewska Aleksandra Maria Staszak Wieslawa Jarmuszkiewicz Ewelina Ratajczak
author_facet	Arleta Małecka Agnieszka Konkolewska Anetta Hanć Liliana Ciszewska Aleksandra Maria Staszak Wieslawa Jarmuszkiewicz Ewelina Ratajczak
author_sort	Arleta Małecka
title	Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
title_short	Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
title_full	Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
title_fullStr	Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
title_full_unstemmed	Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals
title_sort	activation of antioxidative and detoxificative systems in brassica juncea l. plants against the toxicity of heavy metals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d452f4b1ba22436ab3fca54ee485bdba
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Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals

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