Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.

Pseudomonas aeruginosa is a highly adaptable bacterium that thrives in a broad range of ecological niches and can infect multiple hosts as diverse as plants, nematodes and mammals. In humans, it is an important opportunistic pathogen. This wide adaptability correlates with its broad genetic diversit...

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Autores principales:	Silvia Ferrara, Margherita Brugnoli, Angela De Bonis, Francesco Righetti, Francesco Delvillani, Gianni Dehò, David Horner, Federica Briani, Giovanni Bertoni
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d456a7416fa844e5a9de3eb4dc55e4a3
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spelling	oai:doaj.org-article:d456a7416fa844e5a9de3eb4dc55e4a32021-11-18T07:19:11ZComparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.1932-620310.1371/journal.pone.0036553https://doaj.org/article/d456a7416fa844e5a9de3eb4dc55e4a32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22590564/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pseudomonas aeruginosa is a highly adaptable bacterium that thrives in a broad range of ecological niches and can infect multiple hosts as diverse as plants, nematodes and mammals. In humans, it is an important opportunistic pathogen. This wide adaptability correlates with its broad genetic diversity. In this study, we used a deep-sequencing approach to explore the complement of small RNAs (sRNAs) in P. aeruginosa as the number of such regulatory molecules previously identified in this organism is relatively low, considering its genome size, phenotypic diversity and adaptability. We have performed a comparative analysis of PAO1 and PA14 strains which share the same host range but differ in virulence, PA14 being considerably more virulent in several model organisms. Altogether, we have identified more than 150 novel candidate sRNAs and validated a third of them by Northern blotting. Interestingly, a number of these novel sRNAs are strain-specific or showed strain-specific expression, strongly suggesting that they could be involved in determining specific phenotypic traits.Silvia FerraraMargherita BrugnoliAngela De BonisFrancesco RighettiFrancesco DelvillaniGianni DehòDavid HornerFederica BrianiGiovanni BertoniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36553 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Ferrara Margherita Brugnoli Angela De Bonis Francesco Righetti Francesco Delvillani Gianni Dehò David Horner Federica Briani Giovanni Bertoni Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
description	Pseudomonas aeruginosa is a highly adaptable bacterium that thrives in a broad range of ecological niches and can infect multiple hosts as diverse as plants, nematodes and mammals. In humans, it is an important opportunistic pathogen. This wide adaptability correlates with its broad genetic diversity. In this study, we used a deep-sequencing approach to explore the complement of small RNAs (sRNAs) in P. aeruginosa as the number of such regulatory molecules previously identified in this organism is relatively low, considering its genome size, phenotypic diversity and adaptability. We have performed a comparative analysis of PAO1 and PA14 strains which share the same host range but differ in virulence, PA14 being considerably more virulent in several model organisms. Altogether, we have identified more than 150 novel candidate sRNAs and validated a third of them by Northern blotting. Interestingly, a number of these novel sRNAs are strain-specific or showed strain-specific expression, strongly suggesting that they could be involved in determining specific phenotypic traits.
format	article
author	Silvia Ferrara Margherita Brugnoli Angela De Bonis Francesco Righetti Francesco Delvillani Gianni Dehò David Horner Federica Briani Giovanni Bertoni
author_facet	Silvia Ferrara Margherita Brugnoli Angela De Bonis Francesco Righetti Francesco Delvillani Gianni Dehò David Horner Federica Briani Giovanni Bertoni
author_sort	Silvia Ferrara
title	Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
title_short	Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
title_full	Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
title_fullStr	Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
title_full_unstemmed	Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.
title_sort	comparative profiling of pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small rnas.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d456a7416fa844e5a9de3eb4dc55e4a3
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Comparative profiling of Pseudomonas aeruginosa strains reveals differential expression of novel unique and conserved small RNAs.

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