Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells

Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PS...

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Autores principales:	Payungsak Tantipaiboonwong, Wittaya Chaiwangyen, Maitree Suttajit, Napapan Kangwan, Sirichat Kaowinn, Chakkrit Khanaree, Wanisa Punfa, Komsak Pintha
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Perilla frutescens</i> perilla oil perilla seed meal omega-3 rosmarinic acid inflammation Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d457728403724a48a73c0226c19f547f
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spelling	oai:doaj.org-article:d457728403724a48a73c0226c19f547f2021-11-25T18:27:01ZMolecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells10.3390/molecules262267571420-3049https://doaj.org/article/d457728403724a48a73c0226c19f547f2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6757https://doaj.org/toc/1420-3049Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.Payungsak TantipaiboonwongWittaya ChaiwangyenMaitree SuttajitNapapan KangwanSirichat KaowinnChakkrit KhanareeWanisa PunfaKomsak PinthaMDPI AGarticle<i>Perilla frutescens</i>perilla oilperilla seed mealomega-3rosmarinic acidinflammationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6757, p 6757 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Perilla frutescens</i> perilla oil perilla seed meal omega-3 rosmarinic acid inflammation Organic chemistry QD241-441
spellingShingle	<i>Perilla frutescens</i> perilla oil perilla seed meal omega-3 rosmarinic acid inflammation Organic chemistry QD241-441 Payungsak Tantipaiboonwong Wittaya Chaiwangyen Maitree Suttajit Napapan Kangwan Sirichat Kaowinn Chakkrit Khanaree Wanisa Punfa Komsak Pintha Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
description	Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.
format	article
author	Payungsak Tantipaiboonwong Wittaya Chaiwangyen Maitree Suttajit Napapan Kangwan Sirichat Kaowinn Chakkrit Khanaree Wanisa Punfa Komsak Pintha
author_facet	Payungsak Tantipaiboonwong Wittaya Chaiwangyen Maitree Suttajit Napapan Kangwan Sirichat Kaowinn Chakkrit Khanaree Wanisa Punfa Komsak Pintha
author_sort	Payungsak Tantipaiboonwong
title	Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
title_short	Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
title_full	Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
title_fullStr	Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
title_full_unstemmed	Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells
title_sort	molecular mechanism of antioxidant and anti-inflammatory effects of omega-3 fatty acids in perilla seed oil and rosmarinic acid rich fraction extracted from perilla seed meal on tnf-α induced a549 lung adenocarcinoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d457728403724a48a73c0226c19f547f
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Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells

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